Faecal worm egg count analysis for targeting anthelmintic treatment in horses: Points to consider

Equine Veterinary Journal

Volumn 46, Issue 2, 2014, Pages 139-145

  Lester, H E ^a   Matthews, J B ^a  

^a MOREDUN RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Faecal worm egg count; Faecal worm egg count methodology; Horse; Nematode control; Targeted treatment; Variability

Indexed keywords

ANTHELMINTIC AGENT;

ANIMAL; ANIMAL PARASITOSIS; FAECAL WORM EGG COUNT; FAECAL WORM EGG COUNT METHODOLOGY; HORSE; HORSE DISEASE; NEMATODE CONTROL; PARASITE IDENTIFICATION; PARASITOLOGY; REVIEW; TARGETED TREATMENT; VARIABILITY;

FAECAL WORM EGG COUNT; FAECAL WORM EGG COUNT METHODOLOGY; HORSE; NEMATODE CONTROL; TARGETED TREATMENT; VARIABILITY;

ANIMALS; ANTHELMINTICS; HORSE DISEASES; HORSES; PARASITE EGG COUNT; PARASITIC DISEASES, ANIMAL;

EID: 84894226501     PISSN: 04251644     EISSN: 20423306     Source Type: Journal    
DOI: 10.1111/evj.12199     Document Type: Review

References (67)

1. Matthews, J.B. (2008) An update on cyathostomins: anthelmintic resistance and worm control. Equine Vet. Educ. 20, 552-560.
2. Lester, H.E., Spanton, J., Stratford, C.H., Bartley, D.J., Morgan, E.R., Hodgkinson, J.E., Coumbe, K., Mair, T., Swan, B., Lemon, G., Cookson, R. and Matthews, J.B. (2013) Anthelmintic efficacy against cyathostomins in horses in Southern England. Vet. Parasitol. 197, 189-196.
3. Wood, E.D.L., Matthews, J.B., Stevenson, S., Slote, M. and Nussey, D.H. (2012) Variation in fecal egg counts in horses managed for conservation purposes: individual egg shedding consistency, age effects and seasonal variation. Parasitology 140, 115-128.
4. Relf, V.E., Morgan, E.R., Hodgkinson, J.E. and Matthews, J.B. (2013) Helminth excretion with regard to age, gender and management practices on UK Thoroughbred studs. Parasitology 140, 641-652.
5. Drudge, J.H. and Lyons, E.T. (1966) Control of internal parasites of the horse. J. Am. Vet. Med. Ass. 148, 378-383.
6. Reinemeyer, C.R. (2009) Diagnosis and control of anthelmintic-resistant Parascaris equorum. Parasit. Vectors 2, Suppl. 2, S8.
7. Kaplan, R.M. (2002) Anthelmintic resistance in nematodes of horses. Vet. Res. 33, 491-507.
8. Kaplan, R.M. and Nielsen, M.K. (2010) An evidence-based approach to equine parasite control: it ain't the 60s anymore. Equine Vet. Educ. 22, 306-316.
9. Stratford, C.H., Lester, H.E., Pickles, K.J., McGorum, B.C. and Matthews, J.B. (2014) An investigation of anthelmintic efficacy against strongyles on equine yards in Scotland. Equine Vet. J. 46, 17-24.
10. Duncan, J.L. and Love, S. (1991) Preliminary observations on an alternative strategy for the control of horse strongyles. Equine Vet. J. 23, 226-228.
11. Gomez, H.H. and Georgi, J.R. (1991) Equine helminth infections: control by selective chemotherapy. Equine Vet. J. 23, 198-200.
12. Stratford, C.H., McGorum, B.C., Pickles, K.J. and Matthews, J.B. (2011) An update on cyathostomins: anthelmintic resistance and diagnostic tools. Equine Vet. J. 43, 133-139.
13. Lester, H.E., Bartley, D.J., Morgan, E.R., Hodgkinson, J.E., Stratford, C.H. and Matthews, J.B. (2013) A cost comparison of faecal egg count-directed anthelmintic delivery versus interval programme treatments in horses. Vet. Rec. 173, 371.
14. Crofton, H.D. (1971) A quantitative approach to parasitism. Parasitology 62, 179-193.
15. Anderson, R.M. and May, R.M. (1978) Regulation and stability of host-parasite population interactions. Regulatory processes. J. Anim. Ecol. 47, 219-247.
16. Shaw, D.J. and Dobson, A.P. (1995) Patterns of macroparasite abundance and aggregation in wildlife populations: a quantitative review. Parasitology 111, S111-S133.
17. Shaw, D.J. (1998) Patterns of macroparasite aggregation in wildlife host populations. Parasitology 117, 597-610.
18. Calabrese, J.M., Brunner, J.L. and Ostfeld, R.S. (2011) Partitioning the aggregation of parasites on hosts into intrinsic and extrinsic components via an extended poisson-gamma mixture model. PLoS One 6, e29215.
19. Sangster, N. (2003) A practical approach to anthelmintic resistance. Equine Vet. J. 35, 218-219.
20. Uhlinger, C.A. (1993) Uses of fecal egg count data in equine practice. Comp. Cont. Educ. Pract. Vet. 15, 742-748.
21. Larsen, M.L., Ritz, C., Peterson, S.L. and Nielsen, M.K. (2011) Determination of ivermectin efficacy against cyathostomins and Parascaris equorum on horse farms using selective therapy. Vet. J. 188, 44-47.
22. Nielsen, M.K., Haaning, N. and Olsen, S.N. (2006) Strongyle egg shedding consistency in horses on farms using selective therapy in Denmark. Vet. Parasitol. 135, 333-335.
23. Becher, A.M., Mahling, M., Nielsen, M.K. and Pfister, K. (2010) Selective anthelmintic therapy of horses in the Federal states of Bavaria (Germany) and Salzburg (Austria): an investigation into strongyle egg shedding consistency. Vet. Parasitol. 171, 116-122.
24. van Wyk, J.A. (2001) Refugia - overlooked as perhaps the most potent factor concerning the development of anthelmintic resistance. Onderstepoort J. Vet. Res. 68, 55-67.
25. Leathwick, D.M., Waghorn, T.S., Miller, C.M., Candy, P.M. and Oliver, A.M. (2012) Managing anthelmintic resistance-use of a combination anthelmintic and leaving some lambs untreated to slow the development of resistance to ivermectin. Vet. Parasitol. 187, 85-94.
26. Menzel, M., Becher, A.M., Reist, M. and Pfister, K. (2012) Implementation of selective anthelmintic treatment in an equine practice in Upper Bavaria (Germany): 1st experiences. J. Equine Vet. Sci. 32, S35-S36.
27. Dowdall, S.M., Matthews, J.B., Mair, T., Murphy, D., Love, S. and Proudman, C.J. (2002) Antigen-specific IgG(T) responses in natural and experimental cyathostominae infection in horses. Vet. Parasitol. 106, 225-242.
28. Matthews, J.B. (2011) Facing the threat of equine parasitic disease. Equine Vet. J. 43, 126-132.
29. McWilliam, H.E.G., Nisbet, A.J., Dowdall, S.M.J., Hodgkinson, J.E. and Matthews, J.B. (2010) Identification and characterisation of a potential immunodiagnostic marker for larval cyathostominosis. Int. J. Parasitol. 40, 265-275.
30. Beaver, P.C. (1950) The standardization of faecal smears for estimating egg production and worm burden. J. Parasitology 36, 451-456.
31. Gordon, H.M. and Whitlock, H.V. (1939) A new technique for for counting nematode eggs on sheep faeces. J. Counc. Sci. Ind. Res. 12, 50-52.
32. Christie, M. and Jackson, F. (1982) Specific identification of strongyle eggs in small samples of sheep faeces. Res. Vet. Sci. 32, 113-117.
33. Cringoli, G., Rinaldi, L., Maurelli, M.P. and Utzinger, J. (2010) FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nat. Protoc. 5, 503-515.
34. Morrison, D.A. (2004) Technical variability and required sample size of helminth egg isolation procedures: revisited. Parasitol. Res. 94, 361-366.
35. Torgerson, P.R., Paul, M. and Lewis, F.I. (2012) The contribution of random sampling to observed variations in faecal egg counts. Vet. Parasitol. 188, 397-401.
36. Vidyashankar, A.N., Hanlon, B.M. and Kaplan, R.M. (2012) Statistical and biological considerations in evaluating drug efficacy in equine strongyle parasites using fecal egg count data. Vet. Parasitol. 185, 45-56.
37. MAFF (1986) Manual of Veterinary Parasitological Laboratory Techniques, Her Majesty's Stationery Office, London, UK.
38. Coles, G.C., Bauer, C., Borgsteede, F.H., Geerts, S., Klei, T.R., Taylor, M.A. and Waller, P.J. (1992) World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 44, 35-44.
39. Pereckiene, A., Kaziunaite, V., Vysniauskas, A., Petkevicius, S., Malakauskas, A., Sarkūnas, M. and Taylor, M.A. (2007) A comparison of modifications of the McMaster method for the enumeration of Ascaris suum in pig faecal samples. Vet. Parasitol. 149, 111-116.
40. Vadlejch, J., Petrtýl, M., Zaichenko, I., Cadková, Z., Jankovská, I., Langrová, I. and Moravec, M. (2011) Which McMaster egg counting technique is the most relaible? Parasitol. Res. 109, 1387-1397.
41. Morgan, E.R., Cavill, L., Curry, G.E., Wood, R.M. and Mitchell, E.S.E. (2005) Effects of aggregation and sample size on composite faecal egg counts in sheep. Vet. Parasitol. 131, 79-87.
42. Cringoli, G., Rinaldi, L., Veneziano, V., Capelli, G. and Scala, A. (2004) The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dentriticum in sheep. Vet. Parasitol. 123, 121-131.
43. Presland, S.L., Morgan, E.R. and Coles, G.C. (2005) Counting nematode eggs in equine faecal samples. Vet. Rec. 156, 208-210.
44. Bartley, D. and Elsheika, H.M. (2011) Essentials of Veterinary Parasitology, Caister Academic Press, Norfolk, UK. pp 143-148.
45. Egwang, T.G. and Slocombe, J.O. (1982) Evaluation of the Cornell-Wisconsin centrifugal flotation technique for recovering trichostrongylid eggs from bovine faeces. Can. J. Comp. Med. 46, 133-137.
46. Cringoli, G. (2010) FLOTAC, a novel apparatus for a multivalent faecal egg count technique. Parassitologica 48, 381-384.
47. Levecke, B., Rinaldi, L., Charlier, J., Maurelli, M.P., Bosco, A., Vercruysse, J. and Cringoli, G. (2012) The bias, accuracy and precision of faecal egg count reduction test data results in cattle using McMaster, Cornell-Wisconsin and FLOTAC egg counting methods. Vet. Parasitol. 188, 194-199.
48. Rinaldi, L., Coles, G.C., Maurelli, M.P., Musella, V. and Cringoli, G. (2011) Calibration and diagnostic accuracy of simple flotation, McMaster and FLOTAC for parasite egg counts in sheep. Vet. Parasitol. 177, 345-352.
49. Ronsyn, R., Besognet, B., Guerden, T., Benkhelil, A. and Maillard, K. (2012) Evaluation of the Ovatec Plus flotation method as a decision tool for selective anthelmintic treatment against strongyles in horses. Proceedings from Joint Meeting of the Dutch and Belgian Societies for Parasitology, Institute of Tropical Medicine, Antwerp. p. 31.
50. Denwood, M.J., Love, S., Innocent, G.T., Matthews, L., McKendrick, I.J., Hillary, N., Smith, A. and Reid, S.W. (2012) Quantifying the sources of variability in equine faecal egg counts: implications for improving the utility of the method. Vet. Parasitol. 188, 120-126.
51. Mes, T.H.M. (2003) Technical variability and required sample size of helminthegg isolation procedures. Vet. Parasitol. 115, 311-320.
52. Nielsen, M.K., Vidyashankar, A.N., Andersen, U.V., Delisi, K., Pilegaard, K. and Kaplan, R.M. (2010) Effects of fecal collection and storage factors on strongylid egg counts in horses. Vet. Parasitol. 167, 55-61.
53. Lester, H.D., Bartley, D.E., Morgan, E., Hodgkinson, J. and Matthews, J.B. (2012) The spatial distribution of strongyle eggs in horse faeces. J. Equine Vet. Sci. 32, S33-S34.
54. Ogbourne, C.P. (1972) Observations on the free-living stages of strongylid nematodes of horses. Parasitology 64, 461-477.
55. Mfitilodze, M.W. and Hutchinson, G.W. (1987) Development and survival of free-living stages of equine strongyles under laboratory conditions. Vet. Parasitol. 23, 121-133.
56. Denwood, M.J., Reid, S.W.J., Love, S., Nielsen, M.K., Matthews, L., McKendrick, I.J. and Innocent, G.T. (2010) Comparison of three alternative techniques for analysis of equine faecal egg count reduction test data. Prev. Vet. Med. 93, 316-323.
57. Pook, J.F., Power, M.L., Sangster, N.C., Hodgson, J.L. and Hodgson, D.R. (2002) Evaluation of tests for anthelmintic resistance in cyathostomes. Vet. Parasitol. 106, 331-343.
58. Lester, H.E., Bartley, D.J., Morgan, E.R., Hodgkinson, J.E., Stratford, C.H. and Matthews, J.B. (2012) A comparison of three methods for calculating anthelmintic efficacy in equids. J. Equine Vet. Sci. 30, S34-S35.
59. Henriksen, S.A. and Aagaard, K.A. (1976) A simple flotation and McMaster method. Nord. Vet. Med. 28, 392-397.
60. Kassai, T. (1999) Veterinary Helminthology, Butterworth-Heinemann, Oxford.
61. Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. and Jennings, F.W. (1996) Veterinary Parasitology, 2nd edn., Blackwell Science Ltd., Oxford.
62. Gronvold, J. (1991) Laboratory diagnoses of helminths common routine methods used in Denmark. In: Seminars on Parasitic Problems in Farm Animals Related to Fodder Production and Management, Eds: P. Nansen, J. Gronvold and H. Bjorn, The Estonian Academy of Sciences, Tartu, Estonia. pp 47-48.
63. Thienpont, D., Rochette, F. and Vanparijs, O.F.J. (1986) Diagnosing Helminthiasis by Coprological Examination, 2nd edn., Janssen Research Foundatoin, Beerse, Belgium.
64. Wetzel, E. (1951) Verbesserte McMaster-Kammer zum Auszahlen von Wurmeiern. Tierarztl. Umsch. 6, 209-210.
65. Zajicek, D. (1978) Comparison of the efficiency of two quantitative ovoskopic methods (article in Czech). Vet. Med. 23, 275-280.
66. Roepstorff, A. and Nansen, P. (1988) Epidemiology, Diagnosis and Control of Helminth Parasites in Swine, FAO Animal Health Manual, Rome.
67. Breza, M. (1959) The improvement of the swine coprovoscopic examination methodology using a new flotation solution and mucagel (article in Czech). Vet. Cas. 8, 569-576.