Calibration and diagnostic accuracy of simple flotation, McMaster and FLOTAC for parasite egg counts in sheep

Veterinary Parasitology

Volumn 177, Issue 3-4, 2011, Pages 345-352

  Rinaldi, L ^a   Coles, G C ^b   Maurelli, M P ^a   Musella, V ^c   Cringoli, G ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c UNIVERSITY MAGNA GRAECIA OF CATANZARO   (Italy)

Author keywords

Dicrocoelium dendriticum; FLOTAC; Gastrointestinal strongyles; Moniezia expansa; Sheep

Indexed keywords

CHEMICAL COMPOUND; FORMALDEHYDE; MAGNESIUM SULFATE; POTASSIUM IODOMERCURATE; SATURED SODIUM CHLORIDE; SHEATHER SUGAR SOLUTION; SODIUM NITRATE; SUCROSE; UNCLASSIFIED DRUG; ZINC SULFATE;

ARTICLE; CALIBRATION; CONTROLLED STUDY; CRYOPRESERVATION; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DICROCOELIASIS; DICROCOELIUM; DICROCOELIUM DENDRITICUM; FLOATATION METHOD; FLOTAC METHOD; GOLD STANDARD; INTERMETHOD COMPARISON; INTESTINE PARASITE; MCMASTER METHOD; MONIEZIA EXPANSA; MONIEZIASIS; NONHUMAN; PARASITE EXAMINATION; PARASITE IDENTIFICATION; PROCESS OPTIMIZATION; SENSITIVITY AND SPECIFICITY; SHEEP; STORAGE TEMPERATURE; STRONGYLOIDEA; VACUUM; WORM EGG;

ANIMALS; CALIBRATION; FECES; GASTROINTESTINAL DISEASES; PARASITE EGG COUNT; RANDOM ALLOCATION; REPRODUCIBILITY OF RESULTS; SHEEP; SHEEP DISEASES; SPECIMEN HANDLING;

CESTODA; DICROCOELIUM DENDRITICUM; MONIEZIA EXPANSA; NEMATODA; OVIS; OVIS ARIES; STRONGYLES; TREMATODA;

EID: 79954603777     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2010.12.010     Document Type: Article

References (18)

1. Coles G.C., Bauer C., Borgsteede F.H.M., Geerts S., Klei T.R., Taylor M.A., Waller P.J. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 1992, 44:35-44.
2. Cox D.D., Todd A.C. Survey of gastrointestinal parasitism in Wisconsin dairy cattle. J. Am. Vet. Med. Assoc. 1962, 141:706-709.
3. Cringoli G. Flotac, a novel apparatus for multivalent faecal egg count technique. Parassitologia 2006, 48:381-384.
4. Cringoli G., Rinaldi L., Maurelli M.P., Utzinger J. FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nat. Protoc. 2010, 5:503-515.
5. Cringoli G., Rinaldi L., Veneziano V., Capelli G., Scala A. The influence of flotation solution, sample dilution and choice of McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Vet. Parasitol. 2004, 123:121-131.
6. Dudeney A., Campbell C., Coles G.C. Macrocyclic lactone resistance in cyathostomins. Vet. Rec. 2008, 163:163-164.
7. Duthaler U., Rinaldi L., Maurelli M.P., Vargas M., Utzinger J., Cringoli G., Keiser J. Fasciola hepatica: comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats. Exp. Parasitol. 2010, [Epub ahead of print].
8. Egwand T.G., Slocombe J.O. Evaluation of the Cornell-Wisconsin centrifugal flotation technique for recovering trichostrongylid eggs from bovine feces. Can. J. Comp. Med. 1982, 46:133-137.
9. Elliott D.C. Tapeworm (Moniezia expansa) revisited and its effect on sheep production: the evidence reviewed. N. Z. Vet. J. 1986, 34:61-65.
10. Fairweather I. Triclabendazole progress report, 2005-2009: an advancement of learning?. J. Helminthol. 2009, 83:139-150.
11. Fairweather I., Boray J.C. Fasciolicides: efficacy, actions resistance and its management. Vet. J. 1999, 158:81-112.
12. Foreyt W.J. Recovery of nematode eggs and larvae in deer: evaluation of fecal preservation methods. J. Am. Vet. Met. Assoc. 1986, 189:1065-1067.
13. Kaminsky R., Ducray P., Jung M., Clover R., Rufener L., Bouvier J., Weber S.S., Wenger A., Wieland-Berghausen S., Goebel T., Gauvry N., Pautrat F., Skripsky T., Froelich O., Komoin-Oka C., Westlund B., Sluder A., Måser P. A new class of anthelmintics effective against drug-resistant nematodes. Nature 2008, 452:176-180.
14. Kenyon F., Greer A.W., Coles G.C., Cringoli G., Papadopoulos E., Cabaret J., Berrag J., Varady M., Van Wyk J.A., Thomas E., Vercruysse J., Jackson F. The role of targeted selective treatments in the development of refugia-based approaches to the control of gastrointestinal nematodes of small ruminants. Vet. Parasitol. 2009, 164:3-11.
15. Manual of Veterinary Parasitological Techniques 1986, Her Majesty's Stationary Office, London, 160 pp. MAFF (Ed.).
16. Mes T.H., Eysker M., Ploeger H.W. A simple, robust and semi-automated parasite egg isolation protocol. Nat. Prot. 2007, 2:486-489.
17. Presland S.L., Morgan E.R., Coles G.C. Counting nematode eggs in equine faecal samples. Vet. Rec. 2005, 156:208-210.
18. Rinaldi L., Maurelli M.P., Musella V., Santaniello A., Coles G.C., Cringoli G. FLOTAC: an improved method for diagnosis of lungworm infections in sheep. Vet. Parasitol. 2010, 169:395-398.