Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Seijsing, Johan ^a   Lindborg, Malin ^b   Lof̈blom, John ^a   Uhleń, Mathias ^a   Gräslund, Torbjörn ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b Affibody AB   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

FC RECEPTOR; FC RECEPTOR, NEONATAL; GREEN FLUORESCENT PROTEIN; HLA ANTIGEN CLASS 1; HYBRID PROTEIN; IMMOBILIZED ANTIBODY; IMMUNOGLOBULIN FC FRAGMENT; SERUM ALBUMIN;

AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; IMMUNOLOGY; LENTIVIRINAE; NEWBORN; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; SOLUBILITY; TUMOR CELL LINE;

ANTIBODIES, IMMOBILIZED; CELL LINE, TUMOR; CHROMATOGRAPHY, AFFINITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; INFANT, NEWBORN; LENTIVIRUS; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, FC; RECOMBINANT FUSION PROTEINS; SERUM ALBUMIN; SOLUBILITY;

EID: 84894137752     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0081350     Document Type: Article

References (38)

1. Simister NE, Mostov KE (1989) An Fc receptor structurally related to MHC class I antigens. Nature 337: 184-187. doi:10.1038/337184a0. PubMed: 2911353. (Pubitemid 19025381)
2. Burmeister WP, Huber AH, Bjorkman PJ (1994) Crystal structure of the complex of rat neonatal Fc receptor with Fc. Nature 372: 379-383. doi:10.1038/372379a0. PubMed: 7969498. (Pubitemid 24363442)
3. Raghavan M, Gastinel LN, Bjorkman PJ (1993) The class I major histocompatibility complex related Fc receptor shows pH-dependent stability differences correlating with immunoglobulin binding and release. Biochemistry 32: 8654-8660. doi:10.1021/bi00084a037. PubMed: 8357807. (Pubitemid 23270453)
4. Spiekermann GM, Finn PW, Ward ES, Dumont J, Dickinson BL et al. (2002) Receptor-mediated Immunoglobulin G Transport Across Mucosal Barriers in Adult Life: Functional Expression of FcRn in the Mammalian Lung. J Exp Med 196: 303-310. doi:10.1084/jem.20020400. PubMed: 12163559. (Pubitemid 34875660)
5. Borvak J, Richardson J, Medesan C, Antohe F, Radu C et al. (1998) Functional expression of the MHC class I-related receptor, FcRn, in endothelial cells of mice. Int Immunol 10: 1289-1298. doi:10.1093/intimm/10.9.1289. PubMed: 9786428. (Pubitemid 28414334)
6. Kim JK, Tsen MF, Ghetie V, Ward ES (1994) Localization of the site of the murine IgG1 molecule that is involved in binding to the murine intestinal Fc receptor. Eur J Immunol 24: 2429-2434. doi:10.1002/eji.1830241025. PubMed: 7925571. (Pubitemid 24303088)
7. Raghavan M, Bonagura VR, Morrison SL, Bjorkman PJ (1995) Analysis of the pH dependence of the neonatal Fc receptor/immunoglobulin G interaction using antibody and receptor variants. Biochemistry 34: 14649-14657. doi:10.1021/bi00045a005. PubMed: 7578107.
8. Weng Z, Gulukota K, Vaughn DE, Bjorkman PJ, DeLisi C (1998) Computational determination of the structure of rat Fc bound to the neonatal Fc receptor. J Mol Biol 282: 217-225. doi:10.1006/jmbi.1998.2020. PubMed: 9735282. (Pubitemid 28418778)
9. Andersen JT, Dee Qian J, Sandlie I (2006) The conserved histidine 166 residue of the human neonatal Fc receptor heavy chain is critical for the pH-dependent binding to albumin. Eur J Immunol 36: 3044-3051. doi:10.1002/eji.200636556. PubMed: 17048273. (Pubitemid 44782576)
10. Chaudhury C, Brooks CL, Carter DC, Robinson JM, Anderson CL (2006) Albumin binding to FcRn: distinct from the FcRn-IgG interaction. Biochemistry 45: 4983-4990. doi:10.1021/bi052628y. PubMed: 16605266.
11. Leach JL, Sedmak DD, Osborne JM, Rahill B, Lairmore MD et al. (1996) Isolation from human placenta of the IgG transporter, FcRn, and localization to the syncytiotrophoblast: implications for maternal-fetal antibody transport. J Immunol 157: 3317-3322. PubMed: 8871627. (Pubitemid 126449142)
12. Roopenian DC, Akilesh S (2007) FcRn: the neonatal Fc receptor comes of age. Nat Rev Immunol 7: 715-725. doi:10.1038/nri2155. PubMed: 17703228. (Pubitemid 47327396)
13. Montoyo HP, Vaccaro C, Hafner M, Ober RJ, Mueller W et al. (2009) Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice. Proc Natl Acad Sci U S A 106: 2788-2793. doi:10.1073/pnas.0810796106. PubMed: 19188594.
14. Akilesh S (2007) Neonatal FcR expression in bone marrow-derived cells functions to protect serum IgG from catabolism. J Immunol 179: 4580-4588. PubMed: 17878355.
15. Andersen JT, Sandlie I (2009) The versatile MHC class I-related FcRn protects IgG and albumin from degradation: implications for development of new diagnostics and therapeutics. Drug Metab Pharmacokinet 24: 318-332. doi:10.2133/dmpk.24.318. PubMed: 19745559.
16. Andersen JT, Justesen S, Berntzen G, Michaelsen TE, Lauvrak V et al. (2008) A strategy for bacterial production of a soluble functional human neonatal Fc receptor. J Immunol Methods 331: 39-49. doi:10.1016/j.jim.2007.11. 003. PubMed: 18155020.
17. Lee C-H, Choi D-K, Choi H-J, Song M-Y, Kim Y-S (2010) Expression of soluble and functional human neonatal Fc receptor in Pichia pastoris. Protein Expr Purif 71: 42-48. doi:10.1016/j.pep.2009.12.004. PubMed: 20006709.
18. West AP, Bjorkman PJ (2000) Crystal structure and immunoglobulin G binding properties of the human major histocompatibility complex-related Fc receptor. Biochemistry 39: 9698-9708. doi:10.1021/bi000749m. PubMed: 10933786. (Pubitemid 30626819)
19. Berntzen G, Lunde E, Flobakk M, Andersen JT, Lauvrak V et al. (2005) Prolonged and increased expression of soluble Fc receptors, IgG and a TCR-Ig fusion protein by transiently transfected adherent 293E cells. J Immunol Methods 298: 93-104. doi:10.1016/j.jim.2005.01.002. PubMed: 15847800. (Pubitemid 40545096)
20. Magistrelli G, Malinge P, Anceriz N, Desmurs M, Venet S et al. (2012) Robust recombinant FcRn production in mammalian cells enabling oriented immobilization for IgG binding studies. J Immunol Methods 375: 20-29. doi:10.1016/j.jim.2011.09.002. PubMed: 21939661.
21. Feng Y, Gong R, Dimitrov DS (2011) Design, expression and characterization of a soluble single-chain functional human neonatal Fc receptor. Protein Expr Purif 79: 66-71. doi:10.1016/j.pep.2011.03.012. PubMed: 21453773.
22. Zhou J, Johnson JE, Ghetie V, Ober RJ, Sally Ward E (2003) Generation of Mutated Variants of the Human Form of the MHC Class I-related Receptor, FcRn, with Increased Affinity for Mouse Immunoglobulin G. J Mol Biol 332: 901-913. doi:10.1016/S0022-2836(03)00952-5. PubMed: 12972260. (Pubitemid 37101376)
23. Martin WL, West AP, Gan L, Bjorkman PJ (2001) Crystal structure at 2.8 A of an FcRn/heterodimeric Fc complex: mechanism of pH-dependent binding. Mol Cell 7: 867-877. doi:10.1016/S1097-2765(01)00230-1. PubMed: 11336709. (Pubitemid 32436446)
24. Gurbaxani B, Dela Cruz LL, Chintalacharuvu K, Morrison SL (2006) Analysis of a family of antibodies with different half-lives in mice fails to find a correlation between affinity for FcRn and serum half-life. Mol Immunol 43: 1462-1473. doi:10.1016/j.molimm.2005.07.032. PubMed: 16139891. (Pubitemid 43356386)
25. Ober RJ, Radu CG, Ghetie V, Ward ES (2001) Differences in promiscuity for antibody-FcRn interactions across species: implications for therapeutic antibodies. Int Immunol 13: 1551-1559. doi:10.1093/intimm/13.12.1551. PubMed: 11717196. (Pubitemid 33133882)
26. Ober RJ, Martinez C, Vaccaro C, Zhou J, Ward ES (2004) Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn. J Immunol 172: 2021-2029. PubMed: 14764666. (Pubitemid 38182347)
27. Kuo TT, De Muinck EJ, Claypool SM, Yoshida M, Nagaishi T et al. (2009) N-Glycan Moieties in Neonatal Fc Receptor Determine Steady-state Membrane Distribution and Directional Transport of IgG. J Biol Chem 284: 8292-8300. PubMed: 19164298.
28. Suter DM, Cartier L, Bettiol E, Tirefort D, Jaconi ME et al. (2006) Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors. Stem Cells 24: 615-623. doi:10.1634/stemcells.2005-0226. PubMed: 16293575. (Pubitemid 44464765)
29. Tai K, Quintino L, Isaksson C, Gussing F, Lundberg C (2012) Destabilizing domains mediate reversible transgene expression in the brain. PLOS ONE 7: e46269. doi:10.1371/journal.pone.0046269. PubMed: 23029456.
30. Jakobsson J, Ericson C, Jansson M, Björk E, Lundberg C (2003) Targeted transgene expression in rat brain using lentiviral vectors. J Neurosci Res 73: 876-885. doi:10.1002/jnr.10719. PubMed: 12949915. (Pubitemid 37071743)
31. Agoston D, Jakobsson J, Rosenqvist N, Ma K, Lundberg C (2006) Evidence for Disease-Regulated Transgene Expression in the Brain With Use of Lentiviral Vectors. J Neurosci Res 67: 58-67. (Pubitemid 44023063)
32. Zufferey R, Nagy D, Mandel RJ (1997) Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 9: 871-875. PubMed: 9306402.
33. Andersen JT, Daba MB, Berntzen G, Michaelsen TE, Sandlie I (2010) Cross-species binding analyses of mouse and human neonatal Fc receptor show dramatic differences in immunoglobulin G and albumin binding. J Biol Chem 285: 4826-4836. doi:10.1074/jbc.M109.081828. PubMed: 20018855.
34. Bitonti AJ, Dumont JA, Low SC, Peters RT, Kropp KE et al. (2004) Pulmonary delivery of an erythropoietin Fc fusion protein in non-human primates through an immunoglobulin transport pathway. Proc Natl Acad Sci U S A 101: 9763-9768. doi:10.1073/pnas.0403235101. PubMed: 15210944. (Pubitemid 38869310)
35. Yeung YA, Leabman MK, Marvin JS, Qiu J, Adams CW et al. (2009) Engineering human IgG1 affinity to human neonatal Fc receptor: impact of affinity improvement on pharmacokinetics in primates. J Immunol 182: 7663-7671. doi:10.4049/jimmunol.0804182. PubMed: 19494290.
36. Dall'Acqua WF, Kiener PA, Wu H (2006) Properties of human IgG1s engineered for enhanced binding to the neonatal Fc receptor (FcRn). J Biol Chem 281: 23514-23524. doi:10.1074/jbc.M604292200. PubMed: 16793771. (Pubitemid 44274126)
37. Larsson K, Hofström C, Lindskog C, Hansson M, Angelidou P et al. (2011) Novel antigen design for the generation of antibodies to G-protein-coupled receptors. J Immunol Methods 370: 14-23. doi: 10.1016/j.jim.2011.05.001. PubMed: 21605562.
38. Charrier S, Ferrand M, Zerbato M (2011) Quantification of lentiviral vector copy numbers in individual hematopoietic colony-forming cells shows vector dose-dependent effects on the frequency and level of transduction. Gene Ther 5: 479-487. PubMed: 21160533.