Human adipose-derived stromal/stem cell isolation, culture, and osteogenic differentiation

Methods in Enzymology

Volumn 538, Issue , 2014, Pages 67-88

  Qureshi, Ammar T ^a   Chen, Cong ^a   Shah, Forum ^b   Thomas Porch, Caasy ^c   Gimble, Jeffrey M ^b   Hayes, Daniel J ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b LaCell LLC   (United States)

^c TULANE UNIVERSITY   (United States)

Author keywords

Adipose; Cell culture; Ceramic; Composite; Osteogenic differentiation; Scaffold; Stem cell; Stromal

Indexed keywords

OSTEOCALCIN; OSTEONECTIN; OSTEOPONTIN; POLYMER; RNA 18S; TISSUE SCAFFOLD; TRANSCRIPTION FACTOR RUNX2;

ADIPOSE DERIVED STEM CELL; ADIPOSE TISSUE; ADULT STEM CELL; ARTICLE; BONE DEVELOPMENT; CELL ADHESION; CELL COUNT; CELL DENSITY; CELL DIFFERENTIATION; CELL INFILTRATION; CELL ISOLATION; CELL MIGRATION; CELL PROLIFERATION; CELL STRUCTURE; CELL SUSPENSION; CELL VIABILITY; COMPRESSIVE STRENGTH; CRYOPRESERVATION; CYTOTOXICITY ASSAY; DNA CONTENT; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUID TRANSPORT; HUMAN; HUMAN CELL; METABOLIC ACTIVITY ASSAY; OSTEOBLAST; PIPETTE; PRECIPITATION; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; STEM CELL CULTURE; TENSILE STRENGTH; THERMOGRAVIMETRY; TURBULENT FLOW;

ADIPOSE; CELL CULTURE; CERAMIC; COMPOSITE; OSTEOGENIC DIFFERENTIATION; SCAFFOLD; STEM CELL; STROMAL;

ADIPOSE TISSUE; ADULT; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; CERAMICS; CRYOPRESERVATION; HUMANS; OSTEOGENESIS; POLYMERS; STEM CELLS; STROMAL CELLS; TISSUE SCAFFOLDS;

EID: 84894089990     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-800280-3.00005-0     Document Type: Chapter

References (34)

1. P. Bourin, B.A. Bunnell, L. Casteilla, M. Dominici, A.J. Katz, and K.L. March et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: A joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT) Cytotherapy 15 6 2013 641 648 10.1016/j.jcyt.2013.02.006
2. P.P. Carvalho, X. Wu, G. Yu, M. Dietrich, I.R. Dias, and M.E. Gomes et al. Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells Cytotherapy 13 5 2011 594 597 10.3109/14653249.2010.544721
3. M. Dominici, K. Le Blanc, I. Mueller, I. Slaper-Cortenbach, F. Marini, and D. Krause et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement Cytotherapy 8 4 2006 315 317 10.1080/14653240600855905
4. N.O. Fortunel, H.H. Otu, H.H. Ng, J. Chen, X. Mu, and T. Chevassut et al. Comment on "'Stemness': Transcriptional profiling of embryonic and adult stem cells" and "a stem cell molecular signature" Science 302 5644 2003 393 10.1126/science.1086384302/5644/393b [pii], author reply 393
5. L. Garber, C. Chen, K.V. Kilchrist, C. Bounds, J.A. Pojman, and D. Hayes Thiol-acrylate nanocomposite foams for critical size bone defect repair: A novel biomaterial Journal of Biomedical Materials Research. Part A 101 2013 3531 3541 10.1002/jbm.a.34651
6. J.M. Gimble, A.J. Katz, and B.A. Bunnell Adipose-derived stem cells for regenerative medicine Circulation Research 100 9 2007 1249 1260
7. B.C. Goh, S. Thirumala, G. Kilroy, R.V. Devireddy, and J.M. Gimble Cryopreservation characteristics of adipose-derived stem cells: Maintenance of differentiation potential and viability Journal of Tissue Engineering and Regenerative Medicine 1 4 2007 322 324 10.1002/term.35
8. D.W. Hutmacher, J.T. Schantz, C.X.F. Lam, K.C. Tan, and T.C. Lim State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective Journal of Tissue Engineering and Regenerative Medicine 1 4 2007 245 260
9. A.W. James, P. Leucht, B. Levi, A.L. Carre, Y. Xu, and J.A. Helms et al. Sonic Hedgehog influences the balance of osteogenesis and adipogenesis in mouse adipose-derived stromal cells Tissue Engineering. Part A 16 8 2010 2605 2616 10.1089/ten.TEA.2010.0048
10. S.K. Kang, L.A. Putnam, J. Ylostalo, I.R. Popescu, J. Dufour, and A. Belousov et al. Neurogenesis of Rhesus adipose stromal cells Journal of Cell Science 117 18 2004 4289 4299 10.1242/jcs.01264
11. A.J. Katz, R. Llull, M.H. Hedrick, and J.W. Futrell Emerging approaches to the tissue engineering of fat Clinics in Plastic Surgery 26 4 1999 587 603 viii
12. P.J. Kingham, D.F. Kalbermatten, D. Mahay, S.J. Armstrong, M. Wiberg, and G. Terenghi Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro Experimental Neurology 207 2 2007 267 274 10.1016/j.expneurol.2007.06.029
13. B.J. Lawrence, and S.V. Madihally Cell colonization in degradable 3D porous matrices Cell Adhesion & Migration 2 1 2008 9
14. S. Lendeckel, A. Jodicke, P. Christophis, K. Heidinger, J. Wolff, and J.K. Fraser et al. Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: Case report Journal of Cranio-Maxillofacial Surgery 32 6 2004 370 373 10.1016/j.jcms.2004.06.002
15. B. Levi, and M.T. Longaker Concise review: Adipose-derived stromal cells for skeletal regenerative medicine Stem Cells 29 4 2011 576 582 10.1002/stem.612
16. Q. Liu, L. Cen, S. Yin, L. Chen, G. Liu, and J. Chang et al. A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and β-TCP ceramics Biomaterials 29 36 2008 4792 4799 10.1016/j.biomaterials.2008.08.039
17. F. Milat, and K.W. Ng Is Wnt signalling the final common pathway leading to bone formation? Molecular and Cellular Endocrinology 310 1-2 2009 52 62 10.1016/j.mce.2009.06.002
18. H. Miyoshi, T. Ehashi, H. Kawai, N. Ohshima, and S. Suzuki Three-dimensional perfusion cultures of mouse and pig fetal liver cells in a packed-bed reactor: Effect of medium flow rate on cell numbers and hepatic functions Journal of Biotechnology 148 4 2010 226 232 10.1016/j.jbiotec.2010.06. 002
19. A.M. Muller, A. Mehrkens, D.J. Schafer, C. Jaquiery, S. Guven, and M. Lehmicke et al. Towards an intraoperative engineering of osteogenic and vasculogenic grafts from the stromal vascular fraction of human adipose tissue European Cells & Materials 19 2010 127 135
20. S. Partap, N. Plunkett, and F. O'Brien Bioreactors in tissue engineering A. Lazinica, Tissue engineering 2010 IN-TECH Vienna 323 336 (ISBN 978-953-7619-XX)
21. A.T. Qureshi, W.T. Monroe, M.J. Lopez, M.E. Janes, V. Dasa, and S. Park et al. Biocompatible/bioabsorbable silver nanocomposite coatings Journal of Applied Polymer Science 120 5 2011 3042 3053 10.1002/app.33481
22. A.T. Qureshi, L. Terrell, W.T. Monroe, V. Dasa, M.E. Janes, and J.M. Gimble et al. Antimicrobial biocompatible bioscaffolds for orthopaedic implants Journal of Tissue Engineering and Regenerative Medicine 2012 10.1002/term.1532
23. M. Rodbell Metabolism of isolated fat cells. II. The similar effects of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on glucose and amino acid metabolism The Journal of Biological Chemistry 241 1 1966 130 139 [In Vitro]
24. M. Rodbell The metabolism of isolated fat cells. IV. Regulation of release of protein by lipolytic hormones and insulin The Journal of Biological Chemistry 241 17 1966 3909 3917 [In Vitro]
25. M. Rodbell, and A.B. Jones Metabolism of isolated fat cells. 3. The similar inhibitory action of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on lipolysis stimulated by lipolytic hormones and theophylline The Journal of Biological Chemistry 241 1 1966 140 142 [In Vitro]
26. V. Trottier, G. Marceau-Fortier, L. Germain, C. Vincent, and J. Fradette IFATS collection: Using human adipose-derived stem/stromal cells for the production of new skin substitutes Stem Cells 26 10 2008 2713 2723 10.1634/stemcells.2008-0031
27. S. van Gaalen, M. Kruyt, G. Meijer, A. Mistry, A. Mikos, and J. van den Beucken et al. Chapter 19 - Tissue engineering of bone C. van Blitterswijk, P. Thomsen, A. Lindahl, J. Hubbell, D.F. Williams, R. Cancedda, J.D. de Bruijn, J. Sohier, Tissue engineering 2008 Academic Press Burlington, CA 559 610
28. K. Yoshimura, T. Shigeura, D. Matsumoto, T. Sato, Y. Takaki, and E. Aiba-Kojima et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates Journal of Cellular Physiology 208 1 2006 64 76 10.1002/jcp.20636
29. G. Yu, X. Wu, M.A. Dietrich, P. Polk, L.K. Scott, and A.A. Ptitsyn et al. Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes Cytotherapy 12 4 2010 538 546 10.3109/14653241003649528
30. A.S. Zanetti, G.T. McCandless, J.Y. Chan, J.M. Gimble, and D.J. Hayes Characterization of novel akermanite:poly-ε-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering Journal of Tissue Engineering and Regenerative Medicine 2012 10.1002/term.1646
31. A.S. Zanetti, C. Sabliov, J.M. Gimble, and D.J. Hayes Human adipose-derived stem cells and three-dimensional scaffold constructs: A review of the biomaterials and models currently used for bone regeneration Journal of Biomedical Materials Research. Part B, Applied Biomaterials 101 1 2013 187 199 10.1002/jbm.b.32817
32. Z.-Y. Zhang, S.-H. Teoh, M.S.K. Chong, J.T. Schantz, N.M. Fisk, and M.A. Choolani et al. Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells Stem Cells 27 1 2009 126 137 10.1634/stemcells.2008-0456
33. P.A. Zuk, M. Zhu, P. Ashjian, D.A. De Ugarte, J.I. Huang, and H. Mizuno et al. Human adipose tissue is a source of multipotent stem cells Molecular Biology of the Cell 13 12 2002 4279 4295 10.1091/mbc.E02-02-0105
34. P. Zuk, M. Zhu, H. Mizuno, J. Huang, J. Futrell, and A. Katz et al. Multilineage cells from human adipose tissue: Implications for cell-based therapies Tissue Engineering 7 2 2001 211 228