Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells

Cytotherapy

Volumn 13, Issue 5, 2011, Pages 594-597

  Carvalho, Pedro P ^a,b,d   Wu, Xiying ^a   Yu, Gang ^a   Dietrich, Marilyn ^c   Dias, Isabel R ^b,d   Gomes, Manuela E ^b   Reis, Rui L ^b   Gimble, Jeffrey M ^a  

^a PENNINGTON BIOMEDICAL RESEARCH CENTER   (United States)

^b UNIVERSITY OF MINHO   (Portugal)

^c LOUISIANA STATE UNIVERSITY   (United States)

^d UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN; TRYPSIN;

ADIPOSE TISSUE CELL; ADULT; ANIMAL PRODUCT; ARTICLE; BACTERIUM; CELL VIABILITY; CORN; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOPHENOTYPING; PRIORITY JOURNAL; STEM CELL; STROMA CELL;

EID: 79954481571     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.3109/14653249.2010.544721     Document Type: Article

References (25)

1. Gimble JM, Guilak F, Bunnell BA. Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells. Stem Cell Res Ther. 2010;1:19.
2. Lloyd CW, Rees DA, Smith CG, Judge FJ. Mechanisms of cell adhesion: early-forming junctions between aggregating fibroblasts. J Cell Sci. 1976;22:671-84. (Pubitemid 8007676)
3. Barski G. Improved model of an apparatus for trypsinization of tissues for cell cultures. Ann Inst Pasteur (Paris). 1956;91:103-6.
4. Daniel P, Depoux R. Cell dispersion by trypsin at low temperatures. Ann Inst Pasteur (Paris). 1957;92:703-4.
5. Dobrova IN. A simplified method of trypsinization for tissue cell dispersion. Vopr Virusol. 1959;4:241-2.
6. Kuo JF, Holmlund CE, Dill IK. The effect of proteolytic enzymes on isolated adipose cells. Life Sci. 1966;5:2257-62.
7. Shaw WN, Chance RE. Effect of porcine proinsulin in vitro on adipose tissue and diaphragm of the normal rat. Diabetes. 1968;17:737-45.
8. Pozzi E, Zanotto C, Pacchioni S, De Giuli Morghen C, Radaelli A. MHC-restricted cytotoxic T-lymphocyte assay: an improved method based on normal and SV40-immortalized rabbit epidermal target cells. J Virol Methods. 2009;155:77-81.
9. Mesimaki K, Lindroos B, Tornwall J, Mauno J, Lindqvist C, Kontio R, et al. Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells. Int J Oral Maxillofac Surg. 2009;38:201-9.
10. Amos PJ, Kapur SK, Stapor PC, Shang H, Bekiranov S, Khurgel M, et al. Human adipose-derived stromal cells accelerate diabetic wound healing: impact of cell formulation and delivery. Tissue Eng Part A. 2010;16:1595-606.
11. Mattson KJ, Devlin BR, Loskutoff NM. Comparison of a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos. Theriogenology. 2008;69:724-7.
12. Rajala K, Lindroos B, Hussein SM, Lappalainen RS, Pekkanen-Mattila M, Inzunza J, et al. A defined and xeno-free culture method enabling the establishment of clinical-grade human embryonic, induced pluripotent and adipose stem cells. PLoS One. 2010;5:e10246.
13. Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, Haataja R, et al. Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro. Cytotherapy. 2009;11:958-72.
14. Rourou S, van der Ark A, van der Velden T, Kallel H. A microcarrier cell culture process for propagating rabies virus in Vero cells grown in a stirred bioreactor under fully animal component free conditions. Vaccine. 2007;25: 3879-89. (Pubitemid 46613826)
15. Rourou S, van der Ark A, van der Velden T, Kallel H. Development of an animal-component free medium for vero cells culture. Biotechnol Prog. 2009;25:1752-61.
16. Phillips BW, Horne R, Lay TS, Rust WL, Teck TT, Crook JM. Attachment and growth of human embryonic stem cells on microcarriers. J Biotechnol. 2008;138:24-32.
17. Marley MS, Givens MD, Galik PK, Riddell KP, Looney CR, Stringfellow DA. Efficacy of a recombinant trypsin product against bovine herpesvirus 1 associated with in vivo-and in vitro-derived bovine embryos. Theriogenology. 2008;69: 746-57.
18. Panchision DM, Chen HL, Pistollato F, Papini D, Ni HT, Hawley TS. Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24. Stem Cells. 2007;25:1560-70. (Pubitemid 47106026)
19. Mariappan I, Maddileti S, Savy S, Tiwari S, Gaddipati S, Fatima A, et al. In vitro culture and expansion of human limbal epithelial cells. Nat Protoc. 2010;5:1470-9.
20. Hasegawa K, Pomeroy JE, Pera MF. Current technology for the derivation of pluripotent stem cell lines from human embryos. Cell Stem Cell. 2010;6:521-31.
21. Dubois SG, Floyd EZ, Zvonic S, Kilroy G, Wu X, Carling S, et al. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens. Methods Mol Biol. 2008;449:69-79.
22. Bunnell BA, Estes BT, Guilak F, Gimble JM. Differentiation of adipose stem cells. Methods Mol Biol. 2008;456: 155-71.
23. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8:315-7. (Pubitemid 44269669)
24. Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM. Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol. 2001;189:54-63.
25. Yu G WX, Dietrich MA, Polk P, Scott LK, Ptitsyn AA, Gimble JM. Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes. Cytotherapy. 2010;12:538-46.