NS0 cell damage by high gas velocity sparging in protein-free and cholesterol-free cultures

Biotechnology and Bioengineering

Volumn 101, Issue 4, 2008, Pages 751-760

  Zhu, Ying ^a   Cuenca, James V ^a   Zhou, Weichang ^a   Varma, Amit ^a  

^a PDL BioPharma Inc   (United States)

Author keywords

Cell damage; Cholesterol free; Gas sparger; Gas velocity; NS0 cells; Protein free

Indexed keywords

ANIMAL CELL CULTURE; BIOCONVERSION; BIOREACTORS; CARBON DIOXIDE; CELL DEATH; CELL GROWTH; CELLS; CHEMICAL REACTORS; CHOLESTEROL; CONCENTRATION (PROCESS); CYTOLOGY; FLUID DYNAMICS; GASES; GROWTH KINETICS; INDUSTRIAL CHEMICALS; OXYGEN; OXYGENATION; RATE CONSTANTS; SURFACE ACTIVE AGENTS; VEHICULAR TUNNELS; VELOCITY;

ANIMAL CELLS; ANTIBODY MANUFACTURING; ANTIBODY PRODUCTIONS; CARBON DIOXIDE REMOVALS; CASE STUDIES; CELL DAMAGE; CELL DAMAGES; ENTRANCE VELOCITIES; FED BATCHES; FIRST ORDERS; GAS BUBBLES; GAS SPARGING; GAS VELOCITIES; GAS VELOCITY; HIGH CELL DENSITIES; HIGH PRODUCTIVITIES; HYDRODYNAMIC FORCES; IN CELLS; NS0 CELLS; PLURONIC; PROTECTIVE EFFECTS; RECENT DEVELOPMENTS; TROUBLE SHOOTINGS;

CELL CULTURE;

POLOXAMER; SURFACTANT;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOREACTOR; CANCER CELL CULTURE; CELL CULTURE; CELL PROTECTION; CELL STRAIN NS0; CELL VIABILITY; CHOLESTEROL FREE MEDIUM; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; GAS; HYDRODYNAMICS; MOUSE; NONHUMAN; PROTEIN FREE MEDIUM; SPARGED REACTOR; SPARGING; VELOCITY;

ANIMALS; ANTIBODIES; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL PHYSIOLOGY; CELL SURVIVAL; CHOLESTEROL; CULTURE MEDIA, SERUM-FREE; STRESS, MECHANICAL;

ANIMALIA;

EID: 55549108737     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21950     Document Type: Article

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