Rapid cellular phenotyping of human pluripotent stem cell-derived cardiomyocytes using a genetically encoded fluorescent voltage sensor

Stem Cell Reports

Volumn 2, Issue 2, 2014, Pages 163-170

  Leyton Mange, Jordan S ^a   Mills, Robert W ^a   Macri, Vincenzo S ^a   Jang, Min Young ^a   Butte, Faraz N ^a   Ellinor, Patrick T ^a   Milan, David J ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARCLIGHT FUSION PROTEIN; BIOLOGICAL MARKER; CULTURE MEDIUM; HYBRID PROTEIN; PHOTOPROTEIN;

ACTION POTENTIAL; CELL DIFFERENTIATION; CELL LINE; CULTURE MEDIUM; CYTOLOGY; DRUG EFFECTS; EMBRYONIC STEM CELL; GENE EXPRESSION; GENETICS; HEART MUSCLE CELL; HUMAN; METABOLISM; PHARMACOLOGY; PHENOTYPE; PLURIPOTENT STEM CELL; REPORTER GENE; REPRODUCIBILITY; ARTICLE; CARDIOTOXICITY; CELL POPULATION; CONTROLLED STUDY; ELECTRONIC SENSOR; FLUORESCENCE; HEART MUSCLE FIBER MEMBRANE POTENTIAL; HEART MUSCLE POTENTIAL; HEART REPOLARIZATION; HUMAN CELL; MOLECULAR IMAGING; PRIORITY JOURNAL;

ACTION POTENTIALS; BIOMARKERS; CELL DIFFERENTIATION; CELL LINE; CULTURE MEDIA, CONDITIONED; EMBRYONIC STEM CELLS; GENE EXPRESSION; GENES, REPORTER; HUMANS; LUMINESCENT PROTEINS; MYOCYTES, CARDIAC; PHENOTYPE; PLURIPOTENT STEM CELLS; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 84893779887     PISSN: 22136711     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.stemcr.2014.01.003     Document Type: Article

References (25)

1. E. Campeau, V.E. Ruhl, F. Rodier, C.L. Smith, B.L. Rahmberg, J.O. Fuss, J. Campisi, P. Yaswen, P.K. Cooper, and P.D. Kaufman A versatile viral system for expression and depletion of proteins in mammalian cells PLoS ONE 4 2009 e6529
2. G. Cao, J. Platisa, V.A. Pieribone, D. Raccuglia, M. Kunst, and M.N. Nitabach Genetically targeted optical electrophysiology in intact neural circuits Cell 154 2013 904 913
3. G. Chen, D.R. Gulbranson, Z. Hou, J.M. Bolin, V. Ruotti, M.D. Probasco, K. Smuga-Otto, S.E. Howden, N.R. Diol, and N.E. Propson et al. Chemically defined conditions for human iPSC derivation and culture Nat. Methods 8 2011 424 429
4. M.E. Hardy, C.L. Lawrence, N.B. Standen, and G.C. Rodrigo Can optical recordings of membrane potential be used to screen for drug-induced action potential prolongation in single cardiac myocytes? J. Pharmacol. Toxicol. Methods 54 2006 173 182
5. T.J. Herron, P. Lee, and J. Jalife Optical imaging of voltage and calcium in cardiac cells & tissues Circ. Res. 110 2012 609 623
6. I. Itzhaki, L. Maizels, I. Huber, L. Zwi-Dantsis, O. Caspi, A. Winterstern, O. Feldman, A. Gepstein, G. Arbel, and H. Hammerman et al. Modelling the long QT syndrome with induced pluripotent stem cells Nature 471 2011 225 229
7. L. Jin, Z. Han, J. Platisa, J.R. Wooltorton, L.B. Cohen, and V.A. Pieribone Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe Neuron 75 2012 779 785
8. C. Kim, J. Wong, J. Wen, S. Wang, C. Wang, S. Spiering, N.G. Kan, S. Forcales, P.L. Puri, and T.C. Leone et al. Studying arrhythmogenic right ventricular dysplasia with patient-specific iPSCs Nature 494 2013 105 110
9. J.M. Kralj, A.D. Douglass, D.R. Hochbaum, D. Maclaurin, and A.E. Cohen Optical recording of action potentials in mammalian neurons using a microbial rhodopsin Nat. Methods 9 2012 90 95
10. X. Lian, C. Hsiao, G. Wilson, K. Zhu, L.B. Hazeltine, S.M. Azarin, K.K. Raval, J. Zhang, T.J. Kamp, and S.P. Palecek Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling Proc. Natl. Acad. Sci. USA 109 2012 E1848 E1857
11. D.K. Lieu, J.D. Fu, N. Chiamvimonvat, K.C. Tung, G.P. McNerney, T. Huser, G. Keller, C.W. Kong, and R.A. Li Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes Circ. Arrhythm. Electrophysiol. 6 2013 191 201
12. Q. Lou, D.L. Janks, K.M. Holzem, D. Lang, B. Onal, C.M. Ambrosi, V.V. Fedorov, I.W. Wang, and I.R. Efimov Right ventricular arrhythmogenesis in failing human heart: the role of conduction and repolarization remodeling Am. J. Physiol. Heart Circ. Physiol. 303 2012 H1426 H1434
13. E. Matsa, D. Rajamohan, E. Dick, L. Young, I. Mellor, A. Staniforth, and C. Denning Drug evaluation in cardiomyocytes derived from human induced pluripotent stem cells carrying a long QT syndrome type 2 mutation Eur. Heart J. 32 2011 952 962
14. M. Mercola, A. Colas, and E. Willems Induced pluripotent stem cells in cardiovascular drug discovery Circ. Res. 112 2013 534 548
15. T.I. Orbán, A. Apáti, A. Németh, N. Varga, V. Krizsik, A. Schamberger, K. Szebényi, Z. Erdei, G. Várady, and E. Karászi et al. Applying a "double-feature" promoter to identify cardiomyocytes differentiated from human embryonic stem cells following transposon-based gene delivery Stem Cells 27 2009 1077 1087
16. D.M. Roden Drug-induced prolongation of the QT interval N. Engl. J. Med. 350 2004 1013 1022
17. D.M. Roden, and C.C. Hong Stem cell-derived cardiomyocytes as a tool for studying proarrhythmia: a better canary in the coal mine? Circulation 127 2013 1641 1643
18. J. Satin, I. Kehat, O. Caspi, I. Huber, G. Arbel, I. Itzhaki, J. Magyar, E.A. Schroder, I. Perlman, and L. Gepstein Mechanism of spontaneous excitability in human embryonic stem cell derived cardiomyocytes J. Physiol. 559 2004 479 496
19. R.T. Schinzel, T. Ahfeldt, F.H. Lau, Y.K. Lee, A. Cowley, T. Shen, D. Peters, D.H. Lum, and C.A. Cowan Efficient culturing and genetic manipulation of human pluripotent stem cells PLoS ONE 6 2011 e27495
20. S.A. Stewart, D.M. Dykxhoorn, D. Palliser, H. Mizuno, E.Y. Yu, D.S. An, D.M. Sabatini, I.S. Chen, W.C. Hahn, and P.A. Sharp et al. Lentivirus-delivered stable gene silencing by RNAi in primary cells RNA 9 2003 493 501
21. L. Tang, B. Joung, M. Ogawa, P.S. Chen, and S.F. Lin Intracellular calcium dynamics, shortened action potential duration, and late-phase 3 early afterdepolarization in Langendorff-perfused rabbit ventricles J. Cardiovasc. Electrophysiol. 23 2012 1364 1371
22. M. Warren, K.W. Spitzer, B.W. Steadman, T.D. Rees, P. Venable, T. Taylor, J. Shibayama, P. Yan, J.P. Wuskell, L.M. Loew, and A.V. Zaitsev High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS Am. J. Physiol. Heart Circ. Physiol. 299 2010 H1271 H1281
23. Q. Zhang, J. Jiang, P. Han, Q. Yuan, J. Zhang, X. Zhang, Y. Xu, H. Cao, Q. Meng, and L. Chen et al. Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals Cell Res. 21 2011 579 587
24. J. Zhang, M. Klos, G.F. Wilson, A.M. Herman, X. Lian, K.K. Raval, M.R. Barron, L. Hou, A.G. Soerens, and J. Yu et al. Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich method Circ. Res. 111 2012 1125 1136
25. R. Zufferey, D. Nagy, R.J. Mandel, L. Naldini, and D. Trono Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo Nat. Biotechnol. 15 1997 871 875