Hyperspectral unmixing of Raman micro-images for assessment of morphological and chemical parameters in non-dried brain tumor specimens

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 27, 2013, Pages 8719-8728

  Bergner, Norbert ^a   Medyukhina, Anna ^a   Geiger, Kathrin D ^b   Kirsch, Matthias ^b   Schackert, Gabriele ^b   Krafft, Christoph ^a   Popp, Jürgen ^a,c  

^a LEIBNIZ INSTITUTE OF PHOTONIC TECHNOLOGY   (Germany)

^b UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

^c FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Astrocytoma; Brain tumor; Hyperspectral unmixing; Nuclei segmentation; Raman microscopy

Indexed keywords

BRAIN; CLASSIFICATION (OF INFORMATION); COMPUTER AIDED DIAGNOSIS; NUCLEIC ACIDS; PROTEINS; TUMORS;

ASTROCYTOMA; BRAIN TUMORS; HYPERSPECTRAL UNMIXING; NUCLEI SEGMENTATION; RAMAN MICROSCOPY;

IMAGE SEGMENTATION;

LIPID; NUCLEIC ACID; TUMOR MARKER; TUMOR PROTEIN;

ALGORITHM; ARTICLE; ASTROCYTOMA; BRAIN TUMOR; CANCER GRADING; CELL NUCLEUS; CHEMISTRY; GLIOBLASTOMA; HUMAN; IMAGE PROCESSING; ORGANELLE SIZE; PATHOLOGY; RAMAN SPECTROMETRY; REGRESSION ANALYSIS; ULTRASTRUCTURE;

ALGORITHMS; ASTROCYTOMA; BRAIN NEOPLASMS; CELL NUCLEUS; GLIOBLASTOMA; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LEAST-SQUARES ANALYSIS; LIPIDS; NEOPLASM GRADING; NEOPLASM PROTEINS; NUCLEIC ACIDS; ORGANELLE SIZE; SPECTRUM ANALYSIS, RAMAN; TUMOR MARKERS, BIOLOGICAL;

EID: 84893653133     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-7257-7     Document Type: Article

References (29)

1. Campanella R (1992) Membrane lipids modifications in human gliomas of different degree of malignancy. J Neurosurg Sci 36:11-25
2. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P (2007) The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol 114:97-109
3. Krafft C, Kirsch M, Beleites C, Schackert G, Salzer R (2007) Methodology for fiber-optic Raman mapping and FT-IR imaging of metastases in mouse brains. Anal Bioanal Chem 389:1133-1142
4. Kirsch M, Schackert G, Salzer R, Krafft C (2010) Raman spectroscopic imaging for in vivo detection of cerebral brain metastases. Anal Bioanal Chem 398:1707-1713
5. Koljenovic S, Bakker Schut TC, Vincent A, Kros JM, Puppels GJ (2005) Detection of meningeoma in dura mater by Raman spectroscopy. Anal Chem 77:7958-7965
6. Koljenovic S, Choo-Smith LP, Bakker Schut TC, Kros JM, van den Bergh H, Puppels GJ (2002) Discriminating vital tumor from necrotic tissue in human glioblastoma tissue samples by Raman spectroscopy. Lab Invest 82:1265-1277
7. Krafft C, Sobottka SB, Schackert G, Salzer R (2005) Near infrared Raman spectroscopic mapping of native brain tissue and intracranial tumors. Analyst 130:1070-1077
8. Krafft C, Sobottka SB, Schackert G, Salzer R (2006) Raman and infrared spectroscopic mapping of human primary intracranial tumors: A comparative study. J Raman Spectrosc 37:367-375
9. Amharref N, Beljebbar A, Dukic S, Venteo L, Schneider L, Pluot M, Manfait M (2007) Discriminating healthy from tumor and necrosis tissue in rat brain tissue samples by Raman spectral imaging. Biochim Biophys Acta 1768:2605-2615
10. Bergner N, Bocklitz T, Romeike BFM, Reichart R, Kalff R, Krafft C, Popp J (2012) Identification of primary brain tumors of brain metastases by Raman imaging and support vector machines. Chemometr Intell Lab Syst 117:224-232
11. Krafft C, Belay B, Bergner N, Romeike BF, Reichart R, Kalff R, Popp J (2012) Advances in optical biopsy-correlation of malignancy and cell density of primary brain tumors using Raman microspectroscopic imaging. Analyst 137:5533-5537
12. Bergner N, Krafft C, Geiger KD, Kirsch M, Schackert G, Popp J (2012) Unsupervised unmixing of Raman microspectroscopic images for morphological analysis of non-dried brain tumor specimens. Anal Bioanal Chem 403:719-725
13. Medyukhina A, Meyer T, Schmitt M, Romeike BF, Dietzek B, Popp J (2012) Towards automated segmentation of cells and cell nuclei in nonlinear optical microscopy. J Biophotonics 5:878-888
14. Hedegaard M, Matthäus C, Hassing S, Krafft C, Diem M, Popp J (2011) Spectral unmixing and clustering algorithms for assessment of single cells by Raman microscopic imaging. Theor Chem Accounts 130:1249-1260
15. Martens H, Nielsen JP, Engelsen SB (2003) Light scattering and light absorbance separated by extended multiplicative signal correction. Application to near-infrared transmission analysis of powder mixtures. Anal Chem 75:394-404
16. Lawson CL, Hanson RJ (1995) Solving least squares problems, SIAM
17. Meyer T, Bergner N, Medyukhina A, Dietzek B, Krafft C, Romeike BF, Reichart R, Kalff R, Popp J (2012) Interpreting CARS images of tissue within the C-H-stretching region. J Biophotonics 5:729-733
18. Almond LM, Hutchings J, Shepherd N, Barr H, Stone N, Kendall C (2011) Raman spectroscopy: A potential tool for early objective diagnosis of neoplasia in the oesophagus. J Biophotonics 4:685-695
19. Bergholt MS, Zheng W, Ho KY, Teh M, Yeoh KG, So JB, Shabbir A, Huang Z (2013) Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy. J Biophotonics 6:49-59
20. Rowlands CJ, Varma S, PerkinsW, Leach I,Williams H, Notingher I (2012) Rapid acquisition of Raman spectral maps through minimal sampling: Applications in tissue imaging. J Biophotonics 5:220-229
21. Evans CL, Xu X, Kesari S, Xie XS, Wong STC, Young GS (2007) Chemically-selective imaging of brain structures with CARS microscopy. Opt Express 15:12076-12087
22. Freudiger CW, Min W, Saar BG, Lu S, Holtom GR, He C, Tsai JC, Kang JX, Xie XS (2008) Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 322:1857-1861
23. Meyer T, Bergner N, Bielecki C, Krafft C, Akimov D, Romeike BF, Reichart R, Kalff R, Dietzek B, Popp J (2011) Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis. J Biomed Opt 16:021113
24. Baumgartl M, Gottschall T, Abreu-Afonso J, Diez A, Meyer T, Dietzek B, Rothhardt M, Popp J, Limpert J, Tunnermann A (2012) Alignment-free, all-spliced fiber laser source for CARS microscopy based on four-wave-mixing. Opt Express 20:21010-21018
25. Baumgartl M, Chemnitz M, Jauregui C, Meyer T, Dietzek B, Popp J, Limpert J, Tunnermann A (2012) All-fiber laser source for CARS microscopy based on fiber optical parametric frequency conversion. Opt Express 20:4484-4493
26. Chemnitz M, Baumgartl M, Meyer T, Jauregui C, Dietzek B, Popp J, Limpert J, Tunnermann A (2012) Widely tuneable fiber optical parametric amplifier for coherent anti-Stokes Raman scattering microscopy. Opt Express 20:26583-26595
27. Balu M, Liu G, Chen Z, Tromberg BJ, Potma EO (2010) Fiber delivered probe for efficient CARS imaging of tissues. Opt Express 18:2380-2388
28. Murugkar S, Smith B, Srivastava P, Moica A, Naji M, Brideau C, Stys PK, Anis H (2010) Miniaturized multimodal CARS microscope based on MEMS scanning and a single laser source. Opt Express 18:23796-23804
29. Saar BG, Johnston RS, Freudiger CW, Xie XS, Seibel EJ (2011) Coherent Raman scanning fiber endoscopy. Opt Lett 36:2396-2398