Efficient method of genotyping Ob/Ob mice using high resolution melting analysis

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Sutter, Alton G ^a   Palanisamy, Arun P ^a   Kurtz, Nichole ^a   Spyropoulos, Demetri D ^a   Chavin, Kenneth D ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALS; GENOTYPING TECHNIQUES; HETEROZYGOTE; HETEROZYGOTE DETECTION; MICE; MICE, INBRED C57BL; MICE, OBESE; POLYMORPHISM, SINGLE NUCLEOTIDE; TRANSITION TEMPERATURE; WEIGHT GAIN;

EID: 84893588958     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0078840     Document Type: Article

References (16)

1. Malik VS, Willett WC, Hu FB (2013) Global obesity: trends, risk factors and policy implications. Nat Rev Endocrinol 9: 13-27.
2. Trevaskis JL, Butler AA (2005) Double leptin and melanocortin-4 receptor gene mutations have an additive effect on fat mass and are associated with reduced effects of leptin on weight loss and food intake. Endocrinology 146: 4257-4265. (Pubitemid 41324021)
3. Siegmund B, Sennello JA, Lehr HA, Batra A, Fedke I, et al. (2004) Development of intestinal inflammation in double IL-10- and leptin-deficient mice. J Leukoc Biol 76: 782-786. (Pubitemid 39313713)
4. Chehab FF, Lim ME, Lu R (1996) Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin. Nat Genet 12: 318-320. (Pubitemid 26080094)
5. Coleman DL (1979) Obesity genes: beneficial effects in heterozygous mice. Science 203: 663-665.
6. Haller EW, Wittmers LE, Jr., Haller IV, Regal RR (1999) The obese gene is expressed in lean littermates of the genetically obese mouse (C57BL/6J ob/ob). Am J Physiol 276: E762-765. (Pubitemid 29211350)
7. Hirasawa T, Ohara T, Makino S (1997) Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis. Exp Anim 46: 75-78.
8. Chung WK, Chua SC, Lee GH, Leibel RL (1997) Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation. Obes Res 5: 183-185. (Pubitemid 127702721)
9. Namae M, Mori Y, Yasuda K, Kadowaki T, Kanazawa Y, et al. (1998) New method for genotyping the mouse Lep(ob) mutation, using a polymerase chain reaction assay. Lab Anim Sci 48: 103-104. (Pubitemid 128521108)
10. Oler AT, Attie AD (2008) A rapid, microplate SNP genotype assay for the leptinob allele. J Lipid Res 49: 1126-1129.
11. Jackson (2012) Genotyping Protocols for LepOB. The Jackson Laboratory.
12. Sinthuwiwat T, Poowasanpetch P, Wongngamrungroj A, Promso S, Auewarakul C, et al. (2008) High-resolution melting curve analysis for genotyping of common SNP in MTHFR gene using fixed-cell suspension. Mol Cell Probes 22: 329-332.
13. Ellett JD, Evans ZP, Zhang G, Chavin KD, Spyropoulos DD (2009) A rapid PCR-based method for the identification of ob mutant mice. Obesity (Silver Spring) 17: 402-404.
14. Hill HR, Augustine NH, Pryor RJ, Reed GH, Bagnato JD, et al. (2010) Rapid genetic analysis of x-linked chronic granulomatous disease by high-resolution melting. J Mol Diagn 12: 368-376.
15. Chen WJ, Dong WJ, Lin XZ, Lin MT, Murong SX, et al. (2009) Rapid diagnosis of spinal muscular atrophy using high-resolution melting analysis. BMC Med Genet 10: 45.
16. Ong DC, Yam WC, Siu GK, Lee AS (2010) Rapid detection of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis by high-resolution melting analysis. J Clin Microbiol 48: 1047-1054.