Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation

Obesity Research

Volumn 5, Issue 3, 1997, Pages 183-185

  Chung, Wendy K ^a   Chua, Streamson C ^a   Lee, Gwo Hwa ^a   Leibe, Rudolph L ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Lepob; Leptin; ob; Obesity

Indexed keywords

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; ELECTROPHORESIS; GENETICS; GENOTYPE; METHODOLOGY; MOLECULAR GENETICS; MOUSE; MOUSE MUTANT; MUTATION; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

AMINO ACID SEQUENCE; ANIMALS; ELECTROPHORESIS; GENOTYPE; MICE; MICE, OBESE; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH;

EID: 0031131845     PISSN: 10717323     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1550-8528.1997.tb00291.x     Document Type: Article

References (8)

1. Campfield LA, Smith FJ, Guisez Y, Devos R, Burn P. Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks. Science. 1995;269:546-549.
2. Chelab FF, Lim ME, Lu R. Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin. Nat Genet. 1996;12:318-320.
3. Grau O, Griffais R. Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP). Nucleic Acids Res. 1994;22(25):5773-5774.
4. Halaas JL, Gajiwala KS, Maffei M, et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science. 1995;269:543-546.
5. Levin N, Nelson C, Gurney A, Vandlen R, Sauvage FD. Decreased food intake does not completely account for adiposity reduction after ob protein infusion. Proc Natl Acad Sci USA. 1996;93:1726-1730.
6. Pelleymounter MA, Cullen MJ, Baker MB, et al. Effects of the obese product on body weight regulation in ob/ob mice. Science. 1995;269:540-543.
7. Weigle D, Bukowski T, Foster D, et al. Recombinant ob protein reduces feeding and body weight in ob/ob mouse. J. Clin Invest. 1995;96:2065-2070.
8. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994;372:425-432.