2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Kreidenweiss, Andrea ^a   Hopkins, Annika V ^a   Mordmüller, Benjamin ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEINS; CYSTEINE ENDOPEPTIDASES; HELA CELLS; HUMANS; INDOLEACETIC ACIDS; INHIBITORY CONCENTRATION 50; LUMINESCENT PROTEINS; PLASMODIUM FALCIPARUM; PROTEOLYSIS; PROTOZOAN PROTEINS; TRANSCRIPTIONAL ACTIVATION; VIRAL PROTEINS;

EID: 84893580032     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0078661     Document Type: Article

References (16)

1. Meissner M, Krejany E, Gilson PR, de Koning-Ward TF, Soldati D, et al. (2005) Tetracycline analogue-regulated transgene expression in Plasmodium falciparum blood stages using Toxoplasma gondii transactivators. Proc Natl Acad Sci USA 102: 2980-2985. doi:10.1073/pnas.0500112102. (Pubitemid 40299902)
2. Gardner MJ, Hall N, Fung E, White O, Berriman M, et al. (2002) Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419: 498-511. doi:10.1038/nature01097.
3. Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, et al. (2006) Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase. Nat Methods 3: 615-621. doi:10.1038/nmeth904. (Pubitemid 44123428)
4. De Felipe P (2004) Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genet Vaccines Ther 2: 13. doi:10.1186/1479-0556- 2-13.
5. Donnelly ML, Hughes LE, Luke G, Mendoza H, ten Dam E, et al. (2001) The "cleavage" activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring "2A-like" sequences. J Gen Virol 82: 1027-1041. (Pubitemid 32454603)
6. Straimer J, Lee MCS, Lee AH, Zeitler B, Williams AE, et al. (2012) Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases. Nat Meth 9: 993-998. doi:10.1038/nmeth.2143.
7. Armstrong CM, Goldberg DE (2007) An FKBP destabilization domain modulates protein levels in Plasmodium falciparum. Nat Methods 4: 1007-1009. doi:10.1038/nmeth1132. (Pubitemid 350201770)
8. Muralidharan V, Oksman A, Iwamoto M, Wandless TJ, Goldberg DE (2011) Asparagine Repeat Function in a Plasmodium Falciparum Protein Assessed Via a Regulatable Fluorescent Affinity Tag. PNAS 108: 4411-4416. doi:10.1073/pnas. 1018449108.
9. Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M (2009) An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6: 917-922. doi:10.1038/nmeth.1401.
10. Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, et al. (2002) Structure of the Cul1|[ndash]|Rbx1|[ndash]|Skp1|[ndash]|F boxSkp2 SCF ubiquitin ligase complex. Nature 416: 703-709. doi:10.1038/416703a. (Pubitemid 34429140)
11. Trager W, Jensen JB (1976) Human malaria parasites in continuous culture. Science 193: 673-675.
12. Dussurget O, Roulland-Dussoix D (1994) Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera. Appl Environ Microbiol 60: 953-959. (Pubitemid 24077987)
13. Deitsch K, Driskill C, Wellems T (2001) Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes. Nucleic Acids Res 29: 850-853. (Pubitemid 32124398)
14. Tonkin CJ, van Dooren GG, Spurck TP, Struck NS, Good RT, et al. (2004) Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method. Mol Biochem Parasitol 137: 13-21. doi:10.1016/j.molbiopara.2004.05.009. (Pubitemid 38987938)
15. Kreidenweiss A, Kremsner PG, Mordmüller B (2008) Comprehensive study of proteasome inhibitors against Plasmodium falciparum laboratory strains and field isolates from Gabon. Malar J 7: 187. doi:10.1186/1475-2875-7-187.
16. Noedl H, Teja-Isavadharm P, Miller RS (2004) Nonisotopic, semiautomated plasmodium falciparum bioassay for measurement of antimalarial drug levels in serum or plasma. Antimicrob Agents Chemother 48: 4485-4487. doi:10.1128/AAC.48. 11.4485-4487.2004. (Pubitemid 39434925)