The influence of neuronal density and maturation on network activity of hippocampal cell cultures: A methodological study

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Biffi, Emilia ^a,b,c   Regalia, Giulia ^a   Menegon, Andrea ^b   Ferrigno, Giancarlo ^a   Pedrocchi, Alessandra ^a  

^a POLITECNICO DI MILANO   (Italy)

^b SAN RAFFAELE HOSPITAL   (Italy)

^c SCIENTIFIC INSTITUTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALS; CELL COUNT; CELLS, CULTURED; ELECTROPHYSIOLOGICAL PROCESSES; HIPPOCAMPUS; MICE; NERVE NET; NEURONS;

EID: 84893567235     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0083899     Document Type: Article

References (15)

1. Morin F, Takamura Y, Tamiya E (2005) Investigating neuronal activity with planar microelectrode arrays: achievements and new perspectives. J Biosci Bioeng 100: 131-143. (Pubitemid 41573515)
2. Ben-Ari Y (2001) Developing networks play a similar melody. Trends Neurosci 24: 353-360.
3. Chiappalone M, Bove M, Vato A, Tedesco M, Martinoia S (2006) Dissociated cortical networks show spontaneously correlated activity patterns during in vitro development. Brain Res 1093: 41-53. (Pubitemid 43949574)
4. Ichikawa M, Muramoto K, Kobayashi K, Kawahara M, Kuroda Y (1993) Formation and maturation of synapses in primary cultures of rat cerebral cortical cells: An electron microscopic study. Neurosci Res 16: 95-103. (Pubitemid 23111218)
5. Biffi E, Menegon A, Piraino F, Pedrocchi A, Fiore GB et al. (2012) Validation of Long-Term Primary Neuronal Cultures and Network Activity Through the Integration of Reversibly Bonded Microbioreactors and MEA Substrates. Biotechnol Bioeng 109: 166-75.
6. Wagenaar DA, Pine J, Potter SM (2006) An extremely rich repertoire of bursting patterns during the development of cortical cultures. BMC Neurosci 7, 11 DOI:10.1186/1471-2202-7-11.
7. Akum BF, Chen M, Gunderson SI, Riefler GM, Scerri-Hansen MM et al. (2004) Cypin regulates dendrite patterning in hippocampal neurons by promoting microtubule assembly. Nat Neurosci, 7 (2), 145-152. (Pubitemid 38129790)
8. Cullen DK, Gilory M, Irons HR, Laplaca MC (2010) Synapse-to-neuron ratio is inversely related to neuronal density in mature neuronal cultures. Brain Res 1359: 44-55 DOI:10.1016/j.brainres.2010.08.058.
9. Previtera ML, Langhammer CG, Firestein BL (2010) Effects of substrate stiffness and cell density on primary hippocampal cultures. J Biosci Bioeng, 110 (4), 459-470.
10. Ivenshitz M, Segal M (2010). Neuronal density determines network connectivity and spontaneous activity in cultured hippocampus. J Neurophysiol, 104 (2), 1052-1060.
11. Cohen E, Ivenshitz M, Amor-Baroukh V, Greenberger V, Segal M (2008). Determinants of spontaneous activity in networks of cultured hippocampus. Brain Res 1235: 21-30.
12. Rossi S, Muzio L, De Chiara, Grasselli G, Musella A et al. (2011) Impaired striatal GABA transmission in experimental autoimmune encephalomyelitis. Brain Behav Immun 25: 947-56.
13. Biffi E, Regalia G, Ghezzi D, De Ceglia R, Menegon A et al. (2012) A novel environmental chamber for neuronal network multisite recordings. Biotechnol Bioeng 109: 2553-2566.
14. Biffi E, Piraino F, Pedrocchi A, Fiore GB, Ferrigno G et al. (2012) A microfluidic platform for controlled biochemical stimulation of twin neuronal networks. Biomicroflu 6: 24106-2410610.
15. Biffi E, Menegon A, Regalia G, Maida S, Ferrigno G et al. (2011) A new cross-correlation algorithm for the analysis of "in vitro" neuronal network activity aimed at pharmacological studies. J Neurosci Methods 199: 321-327.