Synapse-to-neuron ratio is inversely related to neuronal density in mature neuronal cultures

Brain Research

Volumn 1359, Issue , 2010, Pages 44-55

  Cullen, D Kacy ^a   Gilroy, Meghan E ^b   Irons, Hillary R ^b   Laplaca, Michelle C ^b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b Emory University   (United States)

Author keywords

Cell culture; Cell density; In vitro; Mature; Neuron; Synapse; Synapsin; Tau

Indexed keywords

ANIMAL CELL; ARTICLE; BRAIN CORTEX; CELL CULTURE; CELL DENSITY; CELL FUNCTION; CELL SURVIVAL; CONFOCAL MICROSCOPY; FETUS; IMMUNOCYTOCHEMISTRY; NERVE CELL; NEURITE; NONHUMAN; PRIORITY JOURNAL; RAT; SYNAPSE;

ANIMALS; CELL COUNT; CELLS, CULTURED; CEREBRAL CORTEX; IMMUNOHISTOCHEMISTRY; MICROSCOPY, CONFOCAL; NEURONS; PATCH-CLAMP TECHNIQUES; RATS; SYNAPSES;

EID: 77958020685     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2010.08.058     Document Type: Article

References (42)

1. Y. Ben-Ari, V. Tseeb, D. Raggozzino, R. Khazipov, and J.L. Gaiarsa gamma-Aminobutyric acid (GABA): a fast excitatory transmitter which may regulate the development of hippocampal neurones in early postnatal life Prog. Brain Res. 102 1994 261 273
2. L.I. Binder, A. Frankfurter, and L.I. Rebhun The distribution of tau in the mammalian central nervous system J. Cell Biol. 101 1985 1371 1378
3. M.D. Boehler, B.C. Wheeler, and G.J. Brewer Added astroglia promote greater synapse density and higher activity in neuronal networks Neuron Glia Biol. 3 2007 127 140
4. G.J. Brewer Serum-free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus J. Neurosci. Res. 42 1995 674 683
5. G.J. Brewer, J.R. Torricelli, E.K. Evege, and P.J. Price Optimized survival of hippocampal neurons in B27-supplemented neurobasal, a new serum-free medium combination J. Neurosci. Res. 35 1993 567 576
6. C.R. Cabello, J.J. Thune, H. Pakkenberg, and B. Pakkenberg Ageing of substantia nigra in humans: cell loss may be compensated by hypertrophy Neuropathol. Appl. Neurobiol. 28 2002 283 291
7. L. Cancedda, H. Fiumelli, K. Chen, and M.M. Poo Excitatory GABA action is essential for morphological maturation of cortical neurons in vivo J. Neurosci. 27 2007 5224 5235
8. G. Chen, P.Q. Trombley, and A.N. van den Pol Excitatory actions of GABA in developing rat hypothalamic neurones J. Physiol. 494 Pt 2 1996 451 464
9. D.K. Cullen, and M.C. LaPlaca Neuronal response to high rate shear deformation depends on heterogeneity of the local strain field J. Neurotrauma 23 2006 1304 1319
10. D.K. Cullen, M.C. Lessing, and M.C. Laplaca Collagen-dependent neurite outgrowth and response to dynamic deformation in three-dimensional neuronal cultures Ann. Biomed. Eng. 35 2007 835 846
11. D.K. Cullen, C.M. Simon, and M.C. LaPlaca Strain rate-dependent induction of reactive astrogliosis and cell death in three-dimensional neuronal-astrocytic co-cultures Brain Res. 1158 2007 103 115
12. D.K. Cullen, J. Vukasinovic, A. Glezer, and M.C. Laplaca Microfluidic engineered high cell density three-dimensional neural cultures J. Neural Eng. 4 2007 159 172
13. P. De Camilli, R. Cameron, and P. Greengard Synapsin I (protein I), a nerve terminal-specific phosphoprotein: I. Its general distribution in synapses of the central and peripheral nervous system demonstrated by immunofluorescence in frozen and plastic sections J. Cell Biol. 96 1983 1337 1354
14. J. DeFelipe, L. Alonso-Nanclares, and J.I. Arellano Microstructure of the neocortex: comparative aspects J. Neurocytol. 31 2002 299 316
15. L. Deng, J. Yao, C. Fang, N. Dong, B. Luscher, and G. Chen Sequential postsynaptic maturation governs the temporal order of GABAergic and glutamatergic synaptogenesis in rat embryonic cultures J. Neurosci. 27 2007 10860 10869
16. S.L. Eastwood, P.W. Burnet, B. McDonald, J. Clinton, and P.J. Harrison Synaptophysin gene expression in human brain: a quantitative in situ hybridization and immunocytochemical study Neuroscience 59 1994 881 892
17. S.L. Eastwood, C.S. Weickert, M.J. Webster, M.M. Herman, J.E. Kleinman, and P.J. Harrison Synaptophysin protein and mRNA expression in the human hippocampal formation from birth to old age Hippocampus 16 2006 645 654
18. T.L. Fletcher, P. Cameron, P. De Camilli, and G. Banker The distribution of synapsin I and synaptophysin in hippocampal neurons developing in culture J. Neurosci. 11 1991 1617 1626
19. T.L. Fletcher, P. De Camilli, and G. Banker Synaptogenesis in hippocampal cultures: evidence indicating that axons and dendrites become competent to form synapses at different stages of neuronal development J. Neurosci. 14 1994 6695 6706
20. M. Goedert, R.A. Crowther, and C.C. Garner Molecular characterization of microtubule-associated proteins tau and MAP2 Trends Neurosci. 14 1991 193 199
21. G. Gutierrez-Ospina, E. Uribe-Querol, N. Sanchez, H. Geovannini, P. Padilla, and E. Hernandez-Echeagaray Similar synapse density in layer IV columns of the primary somatosensory cortex of transgenic mice with different brain size: implications for mechanisms underlying the differential allocation of cortical space Brain Behav. Evol. 64 2004 61 69
22. R.S. Hartley, M. Margulis, P.S. Fishman, V.M. Lee, and C.M. Tang Functional synapses are formed between human NTera2 (NT2N, hNT) neurons grown on astrocytes J. Comp. Neurol. 407 1999 1 10
23. M. Ichikawa, K. Muramoto, K. Kobayashi, M. Kawahara, and Y. Kuroda Formation and maturation of synapses in primary cultures of rat cerebral cortical cells: an electron microscopic study Neurosci. Res. 16 1993 95 103
24. H.R. Irons, D.K. Cullen, N.P. Shapiro, N.A. Lambert, R.H. Lee, and M.C. LaPlaca Three-dimensional neural constructs: a novel platform for neurophysiological investigation J. Neural Eng. 5 2008 333 341
25. M.C. LaPlaca, D.K. Cullen, J.J. McLoughlin, and R.S. Cargill II High rate shear strain of three-dimensional neural cell cultures: a new in vitro traumatic brain injury model J. Biomech. 38 2005 1093 1105
26. C. Lesuisse, and L.J. Martin Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death J. Neurobiol. 51 2002 9 23
27. J.W. Mandell, A.J. Czernik, P. De Camilli, P. Greengard, and E. Townes-Anderson Differential expression of synapsins I and II among rat retinal synapses J. Neurosci. 12 1992 1736 1749
28. A. Martin-Pena, A. Acebes, J.R. Rodriguez, A. Sorribes, G.G. de Polavieja, P. Fernandez-Funez, and A. Ferrus Age-independent synaptogenesis by phosphoinositide 3 kinase J. Neurosci. 26 2006 10199 10208
29. R.Y. Moore, and M.E. Bernstein Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity J. Neurosci. 9 1989 2151 2162
30. R.W. Oppenheim Cell death during development of the nervous system Annu. Rev. Neurosci. 14 1991 453 501
31. T.J. O'Shaughnessy, H.J. Lin, and W. Ma Functional synapse formation among rat cortical neurons grown on three-dimensional collagen gels Neurosci. Lett. 340 2003 169 172
32. M. Ouardouz, and B.R. Sastry Activity-mediated shift in reversal potential of GABA-ergic synaptic currents in immature neurons Brain Res. Dev. Brain Res. 160 2005 78 84
33. E. Paizanis, S. Kelai, T. Renoir, M. Hamon, and L. Lanfumey Life-long hippocampal neurogenesis: environmental, pharmacological and neurochemical modulations Neurochem. Res. 32 2007 1762 1771
34. B. Pakkenberg, D. Pelvig, L. Marner, M.J. Bundgaard, H.J. Gundersen, J.R. Nyengaard, and L. Regeur Aging and the human neocortex Exp. Gerontol. 38 2003 95 99
35. F.T. Sayer, M. Oudega, and T. Hagg Neurotrophins reduce degeneration of injured ascending sensory and corticospinal motor axons in adult rat spinal cord Exp. Neurol. 175 2002 282 296
36. G. Seeger, U. Gartner, and T. Arendt Transgenic activation of Ras in neurons increases synapse formation in mouse neocortex J. Neural Transm. 112 2005 751 761
37. R. Steinschneider, P. Delmas, J. Nedelec, M. Gola, D. Bernard, and J. Boucraut Appearance of neurofilament subunit epitopes correlates with electrophysiological maturation in cortical embryonic neurons cocultured with mature astrocytes Brain Res. Dev. Brain Res. 95 1996 15 27
38. L.M. Stone, M.D. Browning, and T.E. Finger Differential distribution of the synapsins in the rat olfactory bulb J. Neurosci. 14 1994 301 309
39. A.N. van den Pol, K. Obrietan, A.B. Belousov, Y. Yang, and H.C. Heller Early synaptogenesis in vitro: role of axon target distance J. Comp. Neurol. 399 1998 541 560
40. J. Vukasinovic, D.K. Cullen, M.C. LaPlaca, and A. Glezer A microperfused incubator for tissue mimetic 3D cultures Biomed. Microdevices 11 2009 1155 1165
41. D.A. Wagenaar, J. Pine, and S.M. Potter An extremely rich repertoire of bursting patterns during the development of cortical cultures BMC Neurosci. 7 2006 11
42. K.E. Waimey, and H.J. Cheng Axon pruning and synaptic development: how are they per-plexin? Neuroscientist 12 2006 398 409