Investigating the feasibility of scale up and automation of human induced pluripotent stem cells cultured in aggregates in feeder free conditions

Journal of Biotechnology

Volumn 173, Issue 1, 2014, Pages 53-58

  Soares, Filipa A C ^a,b   Chandra, Amit ^b   Thomas, Robert J ^b   Pedersen, Roger A ^a   Vallier, Ludovic ^a,c   Williams, David J ^b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b LOUGHBOROUGH UNIVERSITY   (United Kingdom)

^c WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

Automation; Feeder free; Induced pluripotent stem cells; Manufacturing; Scale up

Indexed keywords

CELL-BASED THERAPY; CELLULAR MORPHOLOGY; FEEDER FREE; HUMAN-INDUCED PLURIPOTENT STEM CELLS; INDUCED PLURIPOTENT STEM CELLS; LARGE SCALE PRODUCTIONS; MANUFACTURING FACILITY; SCALE-UP;

AUTOMATION; CELL CULTURE; FEEDING; GENE EXPRESSION; INDUSTRIAL RESEARCH; MANUFACTURE; STEM CELLS;

AGGREGATES;

ARTICLE; AUTOMATION; CELL AGGREGATION; CELL DIFFERENTIATION; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; FEASIBILITY STUDY; GENE EXPRESSION; GERM LAYER; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; LARGE SCALE PRODUCTION; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; STEM CELL EXPANSION;

AUTOMATION; FEEDER FREE; INDUCED PLURIPOTENT STEM CELLS; MANUFACTURING; SCALE UP;

BIOLOGICAL MARKERS; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CULTURE MEDIA; EQUIPMENT DESIGN; GENE EXPRESSION; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICROSCOPY, FLUORESCENCE; ROBOTICS;

EID: 84893428984     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.12.009     Document Type: Article

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