Regio- and stereospecific hydroxylation of various steroids at the 16α position of the D ring by the Streptomyces griseus cytochrome P450 CYP154C3

Applied and Environmental Microbiology

Volumn 80, Issue 4, 2014, Pages 1371-1379

  Makino, Takuya ^a   Katsuyama, Yohei ^a   Otomatsu, Toshihiko ^b   Misawa, Norihiko ^c   Ohnishi, Yasuo ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KNC Bio Research Center   (Japan)

^c ISHIKAWA PREFECTURAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450 MONOOXYGENASES; DEHYDROEPIANDROSTERONE; EQUILIBRIUM DISSOCIATION CONSTANT; ESCHERICHIA COLI CELLS; HIGH RESOLUTION MASS SPECTROMETRY; OXYFUNCTIONALIZATION; STEREOSPECIFIC MANNER; STREPTOMYCES GRISEUS;

BACTERIA; CATALYSIS; DISSOCIATION; ESCHERICHIA COLI; HYDROXYLATION; INDUSTRIAL APPLICATIONS; MASS SPECTROMETRY; PORPHYRINS;

LIPIDS;

BACTERIUM; CATALYSIS; CATALYST; ENZYME ACTIVITY; HYDROXYL RADICAL; OXIDATION; STEROID;

CYTOCHROME P450; RECOMBINANT PROTEIN; STEROID;

ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HYDROXYLATION; KINETICS; MASS SPECTROMETRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; STREPTOMYCES GRISEUS;

CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; HYDROXYLATION; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; RECOMBINANT PROTEINS; STEROIDS; STREPTOMYCES GRISEUS;

EID: 84893417195     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03504-13     Document Type: Article

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