Lipidomic analysis of human tear fl uid reveals structure-specific lipid alterations in dry eye syndrome

Journal of Lipid Research

Volumn 55, Issue 2, 2014, Pages 299-306

  Lam, Sin Man ^a,b   Tong, Louis ^e,f,g,h   Reux, Bastien ^c   Duan, Xinrui ^d   Petznick, Andrea ^e   Yong, Siew Sian ^e   Khee, Cynthia Boo Shiao ^e   Lear, Martin J ^c   Wenk, Markus R ^b,i   Shui, Guanghou ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b Departments of Biological Sciences   (United States)

^c Departments of Chemistry   (United States)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^e SINGAPORE EYE RESEARCH INSTITUTE   (Singapore)

^f SINGAPORE NATIONAL EYE CENTRE   (Singapore)

^g DUKE NUS MEDICAL SCHOOL   (Singapore)

^h Departments of Ophthalmology   (Singapore)

ⁱ NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Mass spectrometry; Meibum; Tear lipidome; Wax esters

Indexed keywords

MASS SPECTROMETRY; MEIBUM; TEAR LIPIDOME; WAX ESTERS;

DRY EYE SYNDROMES; FATTY ACIDS; HUMANS; LIPID METABOLISM; LIPIDS; MEIBOMIAN GLANDS; MOLECULAR WEIGHT; TEARS;

EID: 84893355643     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.P041780     Document Type: Article

References (30)

1. The epidemiology of dry eye disease: report of the Epidemiology Subcommittee of the International Dry Eye WorkShop (2007). Ocul. Surf. 5 : 93-107.
2. Uchino, M., M. Dogru, Y. Yagi, E. Goto, M. Tomita, T. Kon, M. Saiki, Y. Matsumoto, Y. Uchino, N. Yokoi, et al.2006.The features of dry eye disease in a Japanese elderly population. Optom. Vis. Sci. 83 : 797-802.
3. Jie, Y., L. Xu, Y. Y. Wu, and J. B. Jonas.2009.Prevalence of dry eye among adult Chinese in the Beijing Eye Study. Eye (Lond.).23 : 688-693.
4. Sullivan, B. D., L. A. Crews, B. Sönmez, M. F. de la Paz, E. Comert, V. Charoenrook, A. L. de Araujo, J. S. Pepose, M. S. Berg, V. P. Kosheleff, et al.2012.Clinical utility of objective tests for dry eye disease: variability over time and implications for clinical trials and disease management. Cornea.31 : 1000-1008.
5. Foulks, G. N. 2007.The correlation between the tear film lipid layer and dry eye disease. Surv. Ophthalmol. 52 : 369-374.
6. McCulley, J. P., and W. Shine.1997.A compositional based model for the tear film lipid layer. Trans. Am. Ophthalmol. Soc. 95 : 79-88; discussion 88-93.
7. Yokoi, N., H. Yamada, Y. Mizukusa, A. J. Bron, J. M. Tiffany, T. Kato, and S. Kinoshita.2008.Rheology of tear film lipid layer spread in normal and aqueous tear-deficient dry eyes. Invest. Ophthalmol. Vis. Sci. 49 : 5319-5324.
8. Lam, S. M., L. Tong, S. S. Yong, B. Li, S. S. Chaurasia, G. Shui, and M. R. Wenk.2011.Meibum lipid composition in Asians with dry eye disease. PLoS ONE.6 : e24339.
9. Lam, S. M., and G. Shui.2013.Lipidomics as a principal tool for advancing biomedical research. J. Genet. Genomics.40 : 375-390.
10. Dougherty, J. M., J. K. Osgood, and J. P. McCulley.1991.The role of wax and sterol ester fatty acids in chronic blepharitis. Invest. Ophthalmol. Vis. Sci. 32 : 1932-1937.
11. Lam, S. M., L. Tong, X. Duan, A. Petznick, M. R. Wenk, and G. Shui.Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles. J. Lipid Res. 55 : 289-298.
12. Lam, S. M., L. Tong, B. Reux, M. J. Lear, M. R. Wenk, and G. Shui.2013.Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry. J. Chromatogr. A.1308 : 166-171.
13. The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop (2007). Ocul. Surf.5 : 75-92.
14. Borchman, D., G. N. Foulks, M. C. Yappert, and S. E. Milliner.2012. Changes in human meibum lipid composition with age using nuclear magnetic resonance spectroscopy. Invest. Ophthalmol. Vis. Sci. 53 : 475-482.
15. Borchman, D., M. C. Yappert, and G. N. Foulks.2010.Changes in human meibum lipid with meibomian gland dysfunction using principal component analysis. Exp. Eye Res. 91 : 246-256.
16. Borchman, D., G. N. Foulks, and M. C. Yappert.2010.Confirmation of changes in human meibum lipid infrared spectra with age using principal component analysis. Curr. Eye Res. 35 : 778-786.
17. Savini, G., P. Prabhawasat, T. Kojima, M. Grueterich, E. Espana, and E. Goto.2008.The challenge of dry eye diagnosis. Clin. Ophthalmol. 2: 31-55.
18. Nagyová, B., and J. M. Tiffany.1999.Components responsible for the surface tension of human tears. Curr. Eye Res. 19 : 4-11.
19. Glasgow, B. J., A. R. Abduragimov, Z. T. Farahbakhsh, K. F. Faull, and W. L. Hubbell.1995.Tear lipocalins bind a broad array of lipid ligands. Curr. Eye Res. 14 : 363-372.
20. Varela, A. R. P., A. M. P. S. Gonçalves da Silva, A. Fedorov, A. H. Futerman, M. Prieto, and L. C. Silva.2013.Effect of glucosylceramide on the biophysical properties of fluid membranes. Biochim. Biophys. Acta.1828 : 1122-1130.
21. Fleiszig, S. M. J., M. S. F. Kwong, and D. J. Evans.2003.Modification of Pseudomonas aeruginosa interactions with corneal epithelial cells by human tear fluid. Infect. Immun. 71 : 3866-3874.
22. Yokoi, N., F. Mossa, J. M. Tiffany, and A. J. Bron.1999.Assessment of meibomian gland function in dry eye using meibometry. Arch. Ophthalmol. 117 : 723-729.
23. Bron, A. J., and J. M. Tiffany.2004.The contribution of meibomian disease to dry eye. Ocul. Surf. 2 : 149-165.
24. Shimazaki, J., M. Sakata, and K. Tsubota.1995.Ocular surface changes and discomfort in patients with meibomian gland dysfunction. Arch. Ophthalmol. 113 : 1266-1270.
25. Shine, W. E., R. Silvany, and J. P. McCulley.1993.Relation of cholesterol-stimulated Staphylococcus aureus growth to chronic blepharitis. Invest. Ophthalmol. Vis. Sci. 34 : 2291-2296.
26. Pflugfelder, S. C., S. C. Tseng, O. Sanabria, H. Kell, C. G. Garcia, C. Felix, W. Feuer, and B. L. Reis.1998.Evaluation of subjective assessments and objective diagnostic tests for diagnosing tear-film disorders known to cause ocular irritation. Cornea.17 : 38-56.
27. Schuett, B. S., and T. J. Millar.2012.Lipid component contributions to the surface activity of meibomian lipids. Invest. Ophthalmol. Vis. Sci. 53 : 7208-7219.
28. Faheem, S., S. H. Kim, J. Nguyen, S. Neravetla, M. Ball, G. N. Foulks, M. C. Yappert, and D. Borchman.2012.Wax-tear and meibum protein, wax-carotene interactions in vitro using infrared spectroscopy. Exp. Eye Res. 100 : 32-39.
29. Glasgow, B. J., O. K. Gasymov, A. R. Abduragimov, J. J. Engle, and R. C. Casey.2010.Tear lipocalin captures exogenous lipid from abnormal corneal surfaces. Invest. Ophthalmol. Vis. Sci. 51 : 1981-1987.
30. Setälä, N. L., J. M. Holopainen, J. Metso, G. Yohannes, J. Hiidenhovi, L. C. Andersson, O. Eriksson, A. Robciuc, and M. Jauhiainen.2010. Interaction of phospholipid transfer protein with human tear fluid mucins. J. Lipid Res. 51 : 3126-3134.