DNA polymerase preference determines PCR priming efficiency

BMC Biotechnology

Volumn 14, Issue , 2014, Pages

  Pan, Wenjing ^a   Byrne Steele, Miranda ^b   Wang, Chunlin ^c   Lu, Stanley ^d   Clemmons, Scott ^d   Zahorchak, Robert J ^b   Han, Jian ^b  

^a UNIVERSITY OF ALABAMA IN HUNTSVILLE   (United States)

^b HUDSONALPHA INSTITUTE FOR BIOTECHNOLOGY   (United States)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d Diatherix Laboratories   (United States)

Author keywords

DNA polymerase; iC Architect; Next generation sequencing; PCR; Polymerase preference index; PPI; Priming bias

Indexed keywords

DNA; EFFICIENCY; GENE ENCODING; MULTIPLEXING; NUCLEOTIDES; POLYMERIZATION; POLYMERS;

DNA POLYMERASE; IC-ARCHITECT; NEXT-GENERATION SEQUENCING; PCR; POLYMERASE PREFERENCE INDEX; PPI; PRIMING BIAS;

POLYMERASE CHAIN REACTION;

DNA POLYMERASE;

3' UNTRANSLATED REGION; ARTICLE; BASE PAIRING; CONTROLLED STUDY; DNA SEQUENCE; DNA TEMPLATE; GC RICH SEQUENCE; GENE AMPLIFICATION; HIGH THROUGHPUT SEQUENCING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; POLYMERIZATION;

BASE SEQUENCE; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; GENE LIBRARY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; SUBSTRATE SPECIFICITY;

EID: 84893192555     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-10     Document Type: Article

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