Characterization and evaluation of the efficacy of cationic complex mediated plasmid DNA delivery in human embryonic palatal mesenchyme cells

Journal of Tissue Engineering and Regenerative Medicine

Volumn 10, Issue 11, 2016, Pages 927-937

  D'Mello, Sheetal ^a   Salem, Aliasger K ^a   Hong, Liu ^b   Elangovan, Satheesh ^b  

^a UNIVERSITY OF IOWA   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

branched polyethylenimine; calcium phosphate; cytotoxicity; gene delivery; HEPM cells; non viral vectors; plasmid DNA; transfection efficiency

Indexed keywords

BIOMOLECULES; CELL CULTURE; CYTOTOXICITY; DNA; EFFICIENCY; GENES; PROTEINS; VECTORS;

BRANCHED POLYETHYLENIMINE; DNA COMPLEX; EMBRYONICS; GENE DELIVERY; HEPM CELL; N:P RATIO; NONVIRAL VECTORS; PLASMID DNA; POLY(ETHYLENIMINE); TRANSFECTION EFFICIENCY;

GENE TRANSFER;

AGAROSE; CALCIUM PHOSPHATE; GREEN FLUORESCENT PROTEIN; PLASMID DNA; POLYETHYLENEIMINE;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DNA DETERMINATION; EMBRYO; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN EMBRYONIC PALATAL MESENCHYME CELL; IN VITRO STUDY; LIMIT OF QUANTITATION; MESENCHYME CELL; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; OSTEOBLAST; PRIORITY JOURNAL; CELL LINE; CHEMISTRY; CYTOLOGY; EVALUATION STUDY; GENE VECTOR; GENETIC TRANSFECTION; MAMMALIAN EMBRYO; MESODERM; PLASMID; PROCEDURES;

CALCIUM PHOSPHATES; CELL LINE; EMBRYO, MAMMALIAN; GENETIC VECTORS; HUMANS; MESODERM; PLASMIDS; TRANSFECTION;

EID: 84893191924     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.1873     Document Type: Article

References (40)

1. Abdallah B, Hassan A, Benoist C et al. 1996; A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethylenimine. Hum Gene Ther 7: 1947–1954.
2. Anderson WF. 1998; Human gene therapy. Nature 392: 25–30.
3. Battegay EJ, Rupp J, Iruela-Arispe L et al. 1994; PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF β-receptors. J Cell Biol 125: 917–928.
4. Bettinger T, Remy JS, Erbacher P. 1999; Size reduction of galactosylated PEI/DNA complexes improves lectin-mediated gene transfer into hepatocytes. Bioconj Chem 10: 558–561.
5. Bisht S, Bhakta G, Mitra S et al. 2005; pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery. Int J Pharmaceut 288: 157–168.
6. Bolander ME. 1992; Regulation of fracture repair by growth factors. Exp Biol Med 200: 165–170.
7. Bonadio J, Smiley E, Patil P et al. 1999; Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat Med 5: 753–759.
8. Boussif O, Lezoual'h F, Zanta MA et al. 1995; A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A 92: 7297–7301.
9. Dunlap DD, Maggi A, Soria MR et al. 1997; Nanoscopic structure of DNA condensed for gene delivery. Nucleic Acids Res 25: 3095–3101.
10. Elangovan S, Jain S, Tsai PC et al. 2013; Nano-sized calcium phosphate particles for periodontal gene therapy. J Periodontol 84: 117–125.
11. Endo M, Kuroda S, Kondo H et al. 2006; Bone regeneration by modified gene-activated matrix: effectiveness in segmental tibial defects in rats. Tissue Eng 12: 489–497.
12. Fang J, Zhu YY, Smiley E et al. 1996; Stimulation of new bone formation by direct transfer of osteogenic plasmid genes. Proc Natl Acad Sci U S A 93: 5753–5758.
13. Godbey W, Wu K, Hirasaki G et al. 1999a; Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency. Gene Ther 6: 1380–1388.
14. Godbey WT, Wu KK, Mikos AG. 1999b; Poly(ethylenimine) and its role in gene delivery. J Control Release 60: 149–160.
15. Godbey WT, Wu KK, Mikos AG. 1999c; Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. J Biomed Mater Res 45: 268–275.
16. He XX, Wang K, Tan W et al. 2003; Bioconjugated nanoparticles for DNA protection from cleavage. J Am Chem Soc 125: 7168–7169.
17. Horn NA, Meek JA, Budahazi G et al. 1995; Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials. Hum Gene Ther 6: 565–573.
18. Hsu C, Uludag H. 2008; Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers. BMC Biotechnol 8: 23.
19. Intra J, Salem AK. 2008; Characterization of the transgene expression generated by branched and linear polyethylenimine–plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo. J Control Release 130: 129–138.
20. Jordan M, Wurm F. 2004; Transfection of adherent and suspended cells by calcium phosphate. Methods 33: 136–143.
21. Kircheis R, Wightman L, Schreiber A et al. 2001; Polyethylenimine/DNA complexes shielded by transferrin target gene expression to tumors after systemic application. Gene Ther 8: 28–40.
22. Langer R. 1998; Drug delivery and targeting. Nature 392: 5–10.
23. Lieberman JR, Daluiski A, Stevenson S et al. 1999; The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats. J Bone Joint Surg Am 81: 905–917.
24. Liu Y, Wang T, He F et al. 2011; An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery. Int J Nanomed 6: 721.
25. Mulligan R. 1993; The basic science of gene therapy. Science 260: 926–932.
26. Naoto O, Yamaguchi N, Yamaguchi N et al. 1986; The fusogenic effect of synthetic polycations on negatively charged lipid bilayers. J Biochem 100: 935–944.
27. O'Mahoney JV, Adams TE. 1994; Optimization of experimental variables influencing reporter gene expression in hepatoma cells following calcium phosphate transfection. DNA Cell Biol 13: 1227–1232.
28. Ogris M, Steinlein P, Kursa M et al. 1998; The size of DNA/transferrin–PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 5: 1425–1433.
29. Olton D, Li J, Wilson ME et al. 2007; Nanostructured calcium phosphates (NanoCaPs) for non-viral gene delivery: influence of the synthesis parameters on transfection efficiency. Biomaterials 28: 1267–1279.
30. Orrantia E, Chang PL. 1990; Intracellular distribution of DNA internalized through calcium phosphate precipitation. Exp Cell Res 190: 170–174.
31. Parker SE, Vahlsing HL, Serfilippi LM et al. 1995; Cancer gene therapy using plasmid DNA: safety evaluation in rodents and non-human primates. Hum Gene Ther 6: 575–590.
32. Seelos C. 1997; A critical parameter determining the aging of DNA–calcium-phosphate precipitates. Anal Biochem 245: 109–111.
33. Shea LD, Wang D, Francheschi RT et al. 2000; Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds. Tissue Eng 6: 605–617.
34. Sokolova VV, Radtke I, Huemann R et al. 2006; Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles. Biomaterials 27: 3147–3153.
35. Terrell TG, Working PK, Chow CP et al. 1993; Pathology of recombinant human transforming growth factor-β1 in rats and rabbits. Int Rev Exp Pathol 34(pt B): 43–67.
36. Urabe M, Kume A, Tobita K et al. 2000; DNA/calcium phosphate precipitates mixed with medium are stable and maintain high transfection efficiency. Anal Biochem 278: 91–92.
37. Verma IM, Naldini L, Kafri T et al. 2000; Gene therapy: promises, problems and prospects. In Genes and Resistance to Disease, Boulyjenkov V, Berg K, Christen Y (eds). Springer: Berlin, Heidelberg.
38. Welzel T, Radtke I, Meyer-Zaika W et al. 2004; Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA. J Mater Chem 14: 2213–2217.
39. Yoneda T, Pratt RM. 1982; Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J Craniofac Genet Dev Biol 1: 411–423.
40. Zegzula HD, Buck DC, Brekke J et al. 1997; Bone formation with use of rhBMP-2 (recombinant human bone morphogenetic protein-2). J Bone Joint Surg 79: 1778–1790.