Imaging white adipose tissue with confocal microscopy

Methods in Enzymology

Volumn 537, Issue , 2014, Pages 17-30

  Martinez Santibañez, Gabriel ^a   Cho, Kae Won ^b   Lumeng, Carey N ^b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Adipose tissue macrophages; Adipose tissue T cells; Crown like structures; Fat associated lymphoid structure; M1 M2 macrophages; Milky spot

Indexed keywords

ANTIBODY; LIPID;

ADIPOCYTE; ARTICLE; BLOOD VESSEL; CAPILLARY; CELL INTERACTION; CELL STRUCTURE; CONFOCAL LASER MICROSCOPY; LYMPHOID CELL; MACROPHAGE; MICROENVIRONMENT; NONHUMAN; PERFUSION; PRIORITY JOURNAL; STAINING; THREE DIMENSIONAL IMAGING; TISSUE FIXATION; TISSUE STRUCTURE; WHITE ADIPOSE TISSUE;

ADIPOSE TISSUE MACROPHAGES; ADIPOSE TISSUE T CELLS; CROWN-LIKE STRUCTURES; FAT-ASSOCIATED LYMPHOID STRUCTURE; M1/M2 MACROPHAGES; MILKY SPOT;

ADIPOCYTES; ADIPOSE TISSUE, WHITE; CELL DIFFERENTIATION; CELLULAR MICROENVIRONMENT; HUMANS; MACROPHAGES; MICROSCOPY, CONFOCAL; OBESITY; T-LYMPHOCYTES;

EID: 84893174023     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-411619-1.00002-1     Document Type: Chapter

References (34)

1. E. Brakenhielm, R. Cao, B. Gao, B. Angelin, B. Cannon, and P. Parini et al. Angiogenesis inhibitor, TNP-470, prevents diet-induced and genetic obesity in mice Circulation Research 94 12 2004 1579 1588
2. C.H. Cho, Y. Jun Koh, J. Han, H.K. Sung, H. Jong Lee, and T. Morisada et al. Angiogenic role of LYVE-1-positive macrophages in adipose tissue Circulation Research 100 4 2007 e47 e57
3. S. Cinti Adipocyte death defines macrophage localization and function in adipose tissue of obese mice and humans Journal of Lipid Research 46 11 2005 2347 2355
4. S. Cinti, M.C. Zingaretti, R. Cancello, E. Ceresi, and P. Ferrara Morphologic techniques for the study of brown adipose tissue and white adipose tissue Methods in Molecular Biology (Clifton, NJ) 155 2001 21 51
5. D.L. Crandall, G.J. Hausman, and J.G. Kral A review of the microcirculation of adipose tissue: Anatomic, metabolic, and angiogenic perspectives Microcirculation 4 2 1997 211 232
6. D.D. Feng, Y.Y. Tang, H.H. Kwon, H.H. Zong, M.M. Hawkins, and R.N.R. Kitsis et al. High-fat diet-induced adipocyte cell death occurs through a cyclophilin D intrinsic signaling pathway independent of adipose tissue inflammation Diabetes 60 8 2011 2134 2143
7. J.G. Granneman Delivery of DNA into adipocytes within adipose tissue Methods in Molecular Biology (Clifton, NJ) 423 2008 191 195
8. A.S. Greenberg, J.J. Egan, S.A. Wek, N.B. Garty, E.J. Blanchette-Mackie, and C. Londos Perilipin, a major hormonally regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets Journal of Biological Chemistry 266 17 1991 11341 11346
9. J. Han, J.-E. Lee, J. Jin, J.S. Lim, N. Oh, and K. Kim et al. The spatiotemporal development of adipose tissue Development 138 22 2011 5027 5037
10. G.J.G. Hausman, and R.L.R. Richardson Cellular and vascular development in immature rat adipose tissue Journal of Lipid Research 24 5 1983 522 532
11. J.-F. Jasmin, P.G. Frank, and M.P. Lisanti Caveolins and caveolae 2012 Springer New York, NY
12. Y.Y. Kimura, M.M. Ozeki, T.T. Inamoto, and Y.Y. Tabata Time course of de novo adipogenesis in matrigel by gelatin microspheres incorporating basic fibroblast growth factor Tissue Engineering (United States) 8 4 2002 603 613
13. J. Lee, A.B. Goldfine, C. Benoist, S. Shoelson, and D. Mathis Lean, but not obese, fat is enriched for a unique population of regulatory T cells that affect metabolic parameters Nature Medicine 15 8 2009 930 939
14. L. Liu, and M. Meydani Angiogenesis inhibitors may regulate adiposity Nutrition Reviews 61 11 2003 384 387
15. C.N. Lumeng, J.L. Bodzin, and A.R. Saltiel Obesity induces a phenotypic switch in adipose tissue macrophage polarization Journal of Clinical Investigation 117 2007 175 184
16. C.N. Lumeng, J.B. DelProposto, D.J. Westcott, and A.R. Saltiel Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes Diabetes 57 12 2008 3239 3246
17. C.N. Lumeng, S.M. Deyoung, J.L. Bodzin, and A.R. Saltiel Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity Diabetes 56 1 2007 16 23
18. C.N. Lumeng, J. Liu, L. Geletka, C. Delaney, J. Delproposto, and A. Desai et al. Aging is associated with an increase in T cells and inflammatory macrophages in visceral adipose tissue The Journal of Immunology 187 12 2011 6208 6216
19. K. Moro, T. Yamada, M. Tanabe, T. Takeuchi, T. Ikawa, and H. Kawamoto et al. Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid cells Nature 463 7280 2010 540 544
20. D.L. Morris, K.W. Cho, J.L. Delproposto, K.E. Oatmen, L.M. Geletka, and G. Martinez-Santibanez et al. Adipose tissue macrophages function as antigen presenting cells and regulate adipose tissue CD4 + T cells in mice Diabetes 62 8 2013 2762 2772
21. M. Nagasaki, K. Eto, H. Yamashita, M. Ohsugi, and M. Otsu CD8 + effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity Nature Medicine 15 2009 914 920
22. S. Nishimura, I. Manabe, M. Nagasaki, K. Eto, H. Yamashita, and M. Ohsugi et al. CD8 + effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity Nature Medicine 15 8 2009 914 920
23. S. Nishimura, I. Manabe, M. Nagasaki, Y. Hosoya, H. Yamashita, and H. Fujita et al. Adipogenesis in obesity requires close interplay between differentiating adipocytes, stromal cells, and blood vessels Diabetes 56 6 2007 1517 1526
24. S.S. Nishimura, I.I. Manabe, M.M. Nagasaki, K.K. Seo, H.H. Yamashita, and Y.Y. Hosoya et al. In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue Journal of Clinical Investigation 118 2 2008 710 721
25. K. Ohmura, N. Ishimori, Y. Ohmura, S. Tokuhara, A. Nozawa, and S. Horii et al. Natural killer T cells are involved in adipose tissues inflammation and glucose intolerance in diet-induced obese mice Arteriosclerosis, Thrombosis, and Vascular Biology 30 2 2010 193 199
26. S.W. Paddock, and K.W. Eliceiri Laser scanning confocal microscopy: History, applications, and related optical sectioning techniques Methods in Molecular Biology 1075 2014 9 47
27. J. Rangel-Moreno, J.E. Moyron-Quiroz, D.M. Carragher, K. Kusser, L. Hartson, and A. Moquin et al. Omental milky spots develop in the absence of lymphoid tissue-inducer cells and support B and T cell responses to peritoneal antigens Immunity 30 5 2009 731 743
28. M. Spencer, A. Yao-Borengasser, R. Unal, N. Rasouli, C.M. Gurley, and B. Zhu et al. Adipose tissue macrophages in insulin-resistant subjects are associated with collagen VI and fibrosis and demonstrate alternative activation American Journal of Physiology: Endocrinology and Metabolism 299 6 2010 E1016 E1027
29. J.C. Stockert, A. Villanueva, J. Cristóbal, and M. Cañete Improving images of fluorescent cell labeling by background signal subtraction Biotechnic and Histochemistry 84 2 2009 63 68
30. M. Suzuki, Y. Shinohara, Y. Ohsaki, and T. Fujimoto Lipid droplets: Size matters Journal of Electron Microscopy 60 Suppl. 1 2011 S101 S116
31. Y. Tabata, M. Miyao, T. Inamoto, T. Ishii, Y. Hirano, and Y. Yamaoki et al. De novo formation of adipose tissue by controlled release of basic fibroblast growth factor Tissue Engineering (United States) 6 3 2000 279 289
32. H. Tsui, P. Wu, M.G. Davidson, M.N. Alonso, and H.X. Leong B cells promote insulin resistance through modulation of T cells and production of pathogenic IgG antibodies Nature Medicine 17 5 2011 610 617
33. H.H. Xu, G.T.G. Barnes, Q.Q. Yang, G.G. Tan, D.D. Yang, and C.J.C. Chou et al. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance Journal of Clinical Investigation 112 12 2003 1821 1830
34. P.A.P. Zuk, M.M. Zhu, P.P. Ashjian, D.A.D. De Ugarte, J.I.J. Huang, and H.H. Mizuno et al. Human adipose tissue is a source of multipotent stem cells Molecular Biology of the Cell 13 12 2002 4279 4295