Action of a pancreatin and an Aspergillus oryzae protease on whey proteins: Correlation among the methods of analysis of the enzymatic hydrolysates

Brazilian Archives of Biology and Technology

Volumn 56, Issue 6, 2013, Pages 985-995

  Morais, Harriman Aley ^a,b   Silvestre, Marialice Pinto Coelho ^b,c   Silveira, Josianne Nicácio ^b   Silva, Ana Cristina Simões ^b   Silva, Viviane Dias Medeiros ^c   Silva, Mauro Ramalho ^c  

^a UNIVERSIDADE FEDERAL DOS VALES DO JEQUITINHONHA E MUCURI   (Brazil)

^b FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^c EDETEC Industria Alimenticia S/A   (Brazil)

Author keywords

Aspergillus oryzae protease; Degree of hydrolysis; Pancreatin; Peptides; Protein hydrolysates; Whey

Indexed keywords

EID: 84893158028     PISSN: 15168913     EISSN: 16784324     Source Type: Journal    
DOI: 10.1590/S1516-89132013000600014     Document Type: Article

References (47)

1. Adler-Nissen J. Enzymic hydrolysis of food proteins. New York: Elsevier Applied Science Publishers; 1986.
2. AOAC-Association of Official Analytical Chemists. Official Methods of Analysis, 16th ed., Arlington: 1995.
3. Ayres M, Ayres Jr M, Ayres DL, Santos AAS. BioEstat: aplicações estatísticas nas áreas das ciências biológicas e médicas. Belém: Sociedade Civil Mamirauá-MCT-CNPq; 2007 [cited 2012 Mar 15]. Available from: http://www.mamiraua.org.br/ downloads/programas.
4. Benítez R, Ibarz A, Pagan J. Hidrolizados de proteína: procesos y aplicaciones. Acta Bioquím Clín Latinoam. 2008; 42: 227-236.
5. Brans G, Schroën CGPH, van der Sman RGM, Boom RM. Membrane fractionation of milk: state of the art and challenges. J Memb Sci. 2004; 243: 263-272.
6. Briczinski EP, Roberts RF. Production of an exopolysaccharide-containing whey protein concentrate by fermentation of whey. J Dairy Sci. 2002; 85: 3189-3197.
7. Carrasco CA, Guerra M. Lactosuero como fuente de péptidos bioactivos. An Venez Nutr. 2010; 23: 42-49.
8. Carreira RL, Ornellas CBD, Morais HA, Da Motta S, Silvestre MPC. Efeito da precipitação pelo ácido tricloroacético (TCA) e da ultrafiltração sobre o perfil peptídico de hidrolisados de caseína. Cienc Agrotec. 2003; 27: 414-421.
9. Carreira RL, Silva VDM, Lima LG, Morais HA, Silvestre MPC Perfil peptídico de hidrolisados proteicos da farinha de trigo. Pesq Agropec Trop. 2011; 41: 481-489.
10. Cheison SC, Zhang, SB, Wang Z, Xu SY. Comparison of a modified spectrophotometric and the pH-stat methods for determination of the degree of hydrolysis of whey proteins hydrolysed in a tangential-flow filter membrane reactor. Food Res Int. 2009; 42: 91-97.
11. Church FC, Swaisgood HE, Porter DH, Catignani GH. Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J Dairy Sci. 1983; 66: 1219-1227.
12. Denis A, Brambati N, Dessauvages B, Guedj S, Ridoux C, Meffre N, et al. Molecular weight determination of hydrolyzed collagen. Food Hydroc. 2008; 22: 989-994.
13. Doucet D, Otter DE, Gauthier SF, Foegeding EA. Enzyme-induced gelation of extensively hydrolyzed whey proteins by Alcalase: peptide identification and determination of enzyme specificity. J Agric Food Chem. 2003; 51: 6300-6308.
14. Ferreira DF. SISVAR version 5.3 (build 75). Lavras: DEX/UFLA; 2010.
15. Frenhani PB, Burini RC. Mecanismos de absorção de aminoácidos e oligopeptídeos: controle e implicações na dietoterapia humana. Arq Gastroent. 1999; 36: 227-237.
16. Gilbert ER, Wong EA, Webb Jr KE. Peptide absorption and utilization: implications for animal nutrition and health. J Animal Sci. 2008; 86: 1493-1501.
17. Gupta R, Beg QK, Lorenz P. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol. 2002; 59: 15-32.
18. Hartree EF. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972; 48: 422-427.
19. Hinsberger A, Sandhu BK. Digestion and absorption. Curr Paediatrics 2004; 14: 605-611.
20. Hoyle NT, Merritt JH. Quality of fish protein hydrolysates from herring (Clupea harengus). J Food Sci. 1994; 59: 76-79.
21. Levy M. Equilibria of the basic amino acids in the formol titration. J Biol Chem. 1935; 109: 365-381.
22. Lopes DCF, Bizzotto CS, Carreira RL, Afonso WO, Lopes Jr CO, Silvestre MPC. Removal of phenylalanine from protein hydrolysates prepared with rice. J Food Technol. 2008; 6: 57-65.
23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-275.
24. Madureira AR, Pereira CI, Gomes AMP, Pintado ME, Malcata FX. Bovine whey proteins: overview on their main biological properties. Food Res Int. 2007; 40: 1197-1211.
25. Margot A, Flaschel E, Renken A. Continuous monitoring of enzymatic whey protein hydrolysis. correlation of base consumption with soluble nitrogen content. Process Biochem. 1994; 29: 257-262.
26. Morais HA, De Marco LM, Oliveira MC, Silvestre MPC. Casein hydrolysates using papain: peptide profile and encapsulation in liposomes. Acta Alimentaria 2005; 34: 59-69.
27. NC-IUBMB-Nomenclature Committee of the International Union of Biochemistry and Molecular Biology. Enzyme Nomenclature: recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology on the nomenclature and classification of enzymes by the reactions they catalyse. Londres: School of Biological and Chemical Sciences, Queen Mary University; 2010 [cited 2012 Feb 20]. Available from: http://www.chem.qmul.ac.uk/iubmb/enzyme.
28. Nielsen PD, Petersen D, Dambmann C. Improved method for determining food protein degree of hydrolysis. J Food Sci. 2001; 66: 642-646.
29. Otte J, Ju ZY, Skriver A, Qvist KB. Effect of limited proteolysis on the microestructure of heat-induced whey proteins. Int Dairy J. 1996; 79: 782-790.
30. Pacheco MTB, Sgarbieri VC. Effect of different hydrolysates of whey protein on hepatic glutathione content in mice. J Med Food. 2005; 8: 337-342.
31. Page MJ, Di Cera E. Serine peptidases: classification, structure and function. Cell Mol Life Sci. 2008; 65: 1220-1236.
32. Pimentel-Gomes F. Curso de estatística experimental. 14th ed. Piracicaba: Nobel; 2000.
33. Rawlings ND, Barrett AJ, Bateman A. MEROPS: the peptidase database. Nucleic Acids Res. 2010; 38: D227-D233.
34. Roth M. Fluorescence reaction for amino acids. Anal Chem. 1971; 43: 880-882.
35. Rutherford SM. Methodology for determining degree of hydrolysis of proteins in hydrolysates: a review. J AOAC Int. 2010; 93: 1515-1522.
36. Sampaio IBM. Estatística aplicada à experimentação animal. 2rd ed. Belo Horizonte: FEPMVZ; 2002.
37. Silva VDM, Marco LM, Afonso WO, Lopes DCF, Januário JN, Aguiar MJB, et al. Preparation of lowphenylalanine whey hydrolysates using papain and pancreatin immobilized on activated carbon and alumina. Am J Food Technol. 2007; 2: 327-341.
38. Silvestre MPC, Hamon M, Yvon M. Analyses of protein hydrolysates. 1. Use of poly (2-hydroxyethylaspartamide)-silica column in size-exclusion chromatography for the fractionation of casein hydrolysates. J Agric Food Chem. 1994a; 42: 2778-2782.
39. Silvestre MPC, Hamon M, Yvon M. Analyses of protein hydrolysates. 2. Characterization of casein hydrolysates by a rapid peptide quantification method. J Agric Food Chem. 1994b; 42: 2783-2789.
40. Silvestre MPC. Review of methods for the analysis of protein hydrolysates. Food Chem. 1997; 60: 263-271.
41. Smyth M, FitzGerald RJ. Relationship between some characteristics of WPC hydrolysates and the enzyme complement in commercially available proteinase preparations. Int Dairy J. 1998; 8: 819-827.
42. Soares RDL, Capobiango MB, Rolim EA, Silvestre MPC. Enzyme-catalyzed production of oligopeptides from skim milk. Food Biotech. 2007; 21: 45-56.
43. Souza MWS, Biasutti EAR, Carreira RL, Afonso WO, Silva VDM, Silvestre MPC. Obtaining oligopeptides from whey: use of subtilisin and pancreatin. Am J Food Technol. 2008; 3: 315-324.
44. Spellman D, McEvoy E, O'Cuinn G, Fitzgerald RJ. Proteinase and exopeptidase hydrolysis of whey protein: comparasion of the TNBS, OPA e pH-stat method for quantification of degree of hydrolysis. Int Dairy J. 2003; 13: 447-453.
45. Taylor SH. Formol titration: an evaluation of its various modifications. Analyst 1957; 82: 488-498.
46. Wong TM, Boyce COL. Monitoring Proteolysis by Osmometry: A Rapid Method. In: Cherry JP, Barford RA, editors. Methods for protein analysis. Champaign: American Oil Chemists Society; 1988. p. 42-51.
47. Zaia DAM, Zaia CTBV, Lichti J. Determinação de proteínas totais via espectrofotometria: vantagens e desvantagens dos métodos existentes. Quím Nova 1998; 21: 787-793.