Use of sodium lauroyl sarcosinate (sarkosyl) in viable real-time PCR for enumeration of Escherichia coli

Journal of Microbiological Methods

Volumn 98, Issue 1, 2014, Pages 89-93

  Wang, Hui ^a   Gill, Colin O ^a   Yang, Xianqin ^a  

^a LACOMBE RESEARCH CENTRE   (Canada)

Author keywords

Detergent; Escherichia coli; Propidium monoazide; Sarkosyl; Sodium deoxycholate; Verotoxigenic E. coli

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; CHENODEOXYCHOLIC ACID; CHOLIC ACID; DEOXYCHOLATE SODIUM; DETERGENT; GLYCOCHOLATE SODIUM; GLYCODEOXYCHOLIC ACID; LACTIC ACID; PERACETIC ACID; PROPIDIUM MONOAZIDE; SODIUM GLYCOCHENODEOXYCHOLATE; SODIUM LAUROYL SARCOSINATE; SODIUM TAUROCHENODEOXYCHOLATE; TAUROCHOLIC ACID; TAURODEOXYCHOLIC ACID; TRITON X 100; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ISOLATE; CELL COUNTING; CELL MEMBRANE PERMEABILITY; CELL SUSPENSION; CELL VIABILITY; CELLULAR PARAMETERS; CYCLE THRESHOLD; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; WILD TYPE;

DETERGENT; ESCHERICHIA COLI; PROPIDIUM MONOAZIDE; SARKOSYL; SODIUM DEOXYCHOLATE; VEROTOXIGENIC E. COLI;

AZIDES; DEOXYCHOLIC ACID; DNA, BACTERIAL; ESCHERICHIA COLI; LACTIC ACID; PERACETIC ACID; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; SARCOSINE;

EID: 84893085719     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.01.004     Document Type: Article

References (26)

1. Alakomi H.-L., Skyttä E., Saarela M., Mattila-Sandholm T., Latva-Kala K., Helander I.M. Lactic acid permeabilizes Gram-negative bacteria by disrupting the outer membrane. Appl. Environ. Microbiol. 2000, 66:2001-2005.
2. Aslam M., Stanford K., Mcallister T.A. Characterization of antimicrobial resistance and seasonal prevalence of Escherichia coli O157:H7 recovered from commercial feedlots in Alberta, Canada. Lett. Appl. Microbiol. 2010, 50:320-326.
3. Begley M., Gahan C.G., Hill C. The interaction between bacteria and bile. FEMS Microbiol. Rev. 2005, 29:625-651.
4. Bidlack J.E., Silverman P.M. An active type IV secretion system encoded by the F plasmid sensitizes Escherichia coli to bile salts. J. Bacteriol. 2004, 186:5202-5209.
5. Briñez W.J., Roig-Sagués A.X., Hernández Herrero M.M., López-Pedemonte T., Guamis B. Bactericidal efficacy of peracetic acid in combination with hydrogen peroxide against pathogenic and non pathogenic strains of Staphylococcus spp., Listeria spp. and Escherichia coli. Food Control 2006, 17:516-521.
6. Ellebracht J.W., King D.A., Castillo A., Lucia L.M., Acuff G.R., Harris K.B., Savell J.W. Evaluation of peroxyacetic acid as a potential pre-grinding treatment for control of Escherichia coli O157:H7 and Salmonella Typhimurium on beef trimmings. Meat Sci. 2005, 70:197-203.
7. Francois K., Devlieghere F., Standaert A.R., Geeraerd A.H., Cools I., Van Impe J.F., Debevere J. Environmental factors influencing the relationship between optical density and cell count for Listeria monocytogenes. J. Appl. Microbiol. 2005, 99:1503-1515.
8. Gill C.O. Effects on the microbiological condition of product of decontaminating treatments routinely applied to carcasses at beef packing plants. J. Food Prot. 2009, 72:1790-1801.
9. Hofmann A.F., Roda A. Physicochemical properties of bile acids and their relationship to biological properties: an overview of the problem. J. Lipid Res. 1984, 25:1477-1489.
10. Jones T., Gill C.O., Mcmullen L. The behaviour of log phase Escherichia coli at temperatures below the minimum for sustained growth. Food Microbiol. 2002, 19:83-90.
11. Keyhani J., Keyhani E. Increased resistance to detergent in Enterococcus faecalis. Proceedings of the International Conference on Antimicrobial Research 2010, 46-50. World Scientific, Valladolid, Spain. A. Mendez-Vilas (Ed.).
12. King D.A., Lucia L.M., Castillo A., Acuff G.R., Harris K.B., Savell J.W. Evaluation of peroxyacetic acid as a post-chilling intervention for control of Escherichia coli O157:H7 and Salmonella Typhimurium on beef carcass surfaces. Meat Sci. 2005, 69:401-407.
13. Ko J., Hamilton J.A., Ton-Nu H.T., Schteingart C.D., Hofmann A.F., Small D.M. Effects of side chain length on ionization behavior and transbilayer transport of unconjugated dihydroxy bile acids: a comparison of nor-chenodeoxycholic acid and chenodeoxycholic acid. J. Lipid Res. 1994, 35:883-892.
14. Kobayashi H., Oethinger M., Tuohy M.J., Hall G.S., Bauer T.W. Improving clinical significance of PCR: use of propidium monoazide to distinguish viable from dead Staphylococcus aureus and Staphylococcus epidermidis. J. Orthop. Res. 2009, 27:1243-1247.
15. Lee J.-L., Levin R.E. A comparative study of the ability of EMA and PMA to distinguish viable from heat killed mixed bacterial flora from fish fillets. J. Microbiol. Methods 2009, 76:93-96.
16. Lee J.-L., Levin R.E. Discrimination of viable and dead Vibrio vulnificus after refrigerated and frozen storage using EMA, sodium deoxycholate and real-time PCR. J. Microbiol. Methods 2009, 79:184-188.
17. Løvdal T., Hovda M.B., Björkblom B., Møller S.G. Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. J. Microbiol. Methods 2011, 85:164-169.
18. Nocker A., Camper A.K. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microbiol. 2006, 72:1997-2004.
19. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbiol. Methods 2006, 67:310-320.
20. Nocker A., Sossa-Fernandez P., Burr M.D., Camper A.K. Use of propidium monoazide for live/dead distinction in microbial ecology. Appl. Environ. Microbiol. 2007, 73:5111-5117.
21. Nocker A., Sossa K.E., Camper A.K. Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. J. Microbiol. Methods 2007, 70:252-260.
22. Shi H., Xu W., Luo Y., Chen L., Liang Z., Zhou X., Huang K. The effect of various environmental factors on the ethidium monoazide and quantitative PCR method to detect viable bacteria. J. Appl. Microbiol. 2011, 111:1194-1204.
23. Thanassi D.G., Cheng L.W., Nikaido H. Active efflux of bile salts by Escherichia coli. J. Bacteriol. 1997, 179:2512-2518.
24. USDA Safe and suitable ingredients used in the production of meat, poultry, and egg products Available at:, (Accessed August 10, 2013). http://www.fsis.usda.gov/OPPDE/rdad/FSISDirectives/7120.1.pdf.
25. Yang X., Badoni M., Gill C.O. Use of propidium monoazide and quantitative PCR for differentiation of viable Escherichia coli from E. coli killed by mild or pasteurizing heat treatments. Food Microbiol. 2011, 28:1478-1482.
26. Yang X., Badoni M., Youssef M.K., Gill C.O. Enhanced control of microbiological contamination of product at a large beef packing plant. J. Food Prot. 2012, 75:144-149.