Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:Diacylglycerol acyltransferase activity

Applied and Environmental Microbiology

Volumn 80, Issue 3, 2014, Pages 1132-1141

  Villa, Juan A ^a,b   Cabezas, Matilde ^a   de la Cruz, Fernando ^a   Moncalián, Gabriel ^a  

^a University of Cantabria   (Spain)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE SCANNING MUTANTS; C-TERMINAL DOMAINS; DIACYLGLYCEROL ACYLTRANSFERASES; ENERGY-STORAGE MOLECULE; LIMITED PROTEOLYSIS; SEQUENCE ALIGNMENTS; STRUCTURAL MODELING; STRUCTURAL UNDERSTANDING;

AMINO ACIDS; ENZYMES; ESTERIFICATION; ESTERS; GLYCEROL; LIPIDS; PROTEINS; PROTEOLYSIS; SYNTHESIS (CHEMICAL);

PLANTS (BOTANY);

BACTERIUM; CATALYSIS; ENZYME ACTIVITY; ESTER; LIPID; MUTATION; PEPTIDE;

ACYL COENZYME A; ACYLTRANSFERASE; DIACYLGLYCEROL; DIACYLGLYCEROL ACYLTRANSFERASE; LONG CHAIN ALCOHOL O FATTY ACYLTRANSFERASE; LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE;

ACTINOBACTERIA; AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEOBACTERIA;

ACTINOBACTERIA; ACYL COENZYME A; ACYLTRANSFERASES; AMINO ACID SEQUENCE; DIACYLGLYCEROL O-ACYLTRANSFERASE; DIGLYCERIDES; DNA MUTATIONAL ANALYSIS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEOBACTERIA; PROTEOLYSIS;

EID: 84893013220     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03433-13     Document Type: Article

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