Both histidine residues of the conserved HHXXXDG motif are essential for wax ester synthase/acyl-CoA:Diacylglycerol acyltransferase catalysis

European Journal of Lipid Science and Technology

Volumn 111, Issue 2, 2009, Pages 112-119

  Stöveken, Tim ^a,b   Kalscheuer, Rainer ^a,c   Steinbüchel, Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b MERZ PHARMACEUTICALS GMBH   (Germany)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Acyltransferase; HHXXXDG motif; WS DGAT

Indexed keywords

ACINETOBACTER; ACINETOBACTER BAYLYI; BACTERIA (MICROORGANISMS);

EID: 68349111234     PISSN: 14387697     EISSN: 14389312     Source Type: Journal    
DOI: 10.1002/ejlt.200800167     Document Type: Article

References (21)

1. R. Kalscheuer, A. Steinbüchel: A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1. J Biol Chem. 2003, 278, 8075-8082.
2. J. Daniel, C. Deb, V. S. Dubey, T. D. Sirakova, B. Abomoelak, H. R. Morbidoni, P. E. Kolattukudy: Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancylike state in culture. J Bacteriol. 2004, 186, 5017-5030.
3. M. Wältermann, T. Stöveken, A. Steinbüchel: Key enzymes of neutral lipid storage compounds in prokaryotes: properties, function and occurrence of wax ester synthases/acyl-CoA:diacylglycerol acyltransferases. Biochimie. 2007, 89, 230-242.
4. E. Holtzapple, C. Schmidt-Dannert: Biosynthesis of isoprenoid wax ester in Marinobacter hydrocarbonoclusticus DSM 8798: Identification and characterization of isoprenoid coenzyme A synthetase and wax ester synthases. J Bacteriol. 2007, 189, 3804-3812.
5. R. Kalscheuer, T. Stöveken, U. Malkus, R. Reichelt, P. N. Golyshin, .J. S. Sabirova, M. Ferrer, K. N. Timmis, A. Steinbüchel: Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria. J Bacteriol. 2007, 189, 918-928.
6. N. J. Garton, H. Christensen, D. E. Minnikin, R. A. Adegbola, M. R. Barer: Intracellular lipophilic inclusions of mycobacteria in vitro and in sputum. Microbiology 2002, 148, 2951-2958.
7. T. D. Sirakova, V. S. Dubey, C. Deb, J. Daniel, T. A. Korotkova, B. Abomoelak, P. E. Kolattukudy: Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress. Microbiology. 2006, 152, 2717-2725.
8. A. King, J.W. Nam, J. Han, J. Hilliard, J.G. Jaworski: Cuticular wax biosynthesis in petunia petals: Cloning and characterization of an alcohol-acyltransferase that synthesizes wax-esters. Planta. 2007, 226, 381-394.
9. R. Kalscheuer, H. Luftmann, A. Steinbüchel: Synthesis of novel lipids in Saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase. Appl Environ Microbiol. 2004, 70, 7119-7125.
10. R. Kalscheuer, T. Stölting, A. Steinbüchel: Microdiesel: Escherichia coli engineered for fuel production. Microbiology. 2006, 152, 2529-2536.
11. A. G. W. Leslie, P. C. E. Moody, W. V. Shaw: Structure of chloramphenicol acetyltransferase at 1.75-Å resolution. Proc Natl Acad Sci USA. 1988, 85, 4133-4137.
12. V. Bergendahl, U. Linne, A. Marahiel: Mutational analysis of the C-domain in nonribosomal peptide synthesis. Eur J Biochem. 2002, 269, 620-629.
13. J. Buglino, K. C. Onwueme, J. A. Ferreras, L. E. Quadri, C.D. Lima: Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. J Biol Chem. 2004, 16, 30634-30642.
14. K. C. Onwueme, J. A. Ferreras, J. Buglino, C. D. Lima, L. E. N. Quari: Mycobacterial polyketide-associated proteins are acyltransferases: Proof of principle with Mycobacterium tuberculosis PapA5. Proc Natl Acad Sci USA. 2004, 110, 4608- 4613.
15. T. Stöveken, R. Kalscheuer, U. Malkus, R. Reichelt, A. Steinbüchel: The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: Characterization of a novel type of acyltransferase. J Bacteriol. 2005, 187, 1369-1376.
16. S. Uthoff, T. Stöveken, N. Weber, K. Vosmann, E. Klein, R. Kalscheuer, A. Steinbüchel: Thio wax ester biosynthesis uti-lizing the unspecific bifunctional wax ester synthase/diacylglycerol acyltransferase of Acinetobacter sp. strain ADP1. Appl Environ Microbiol. 2005, 71, 790-796.
17. P. Kumar, M.W. Schelle, M. Jain, F. L. Lin, C. J. Petzhold, M. D. Leavell, J. A. Leary, J. S. Cox, C. R. Bertozzi: PapA1 and PapA2 are acyltransferases essential for the biosynthesis of the Mycobacterium tuberculosis virulence factor sulfolipid-1. Proc Natl Acad Sci USA. 2007, 27, 11221-11226.
18. S. N. Ho, H. D. Hunt, R. M. Horton, J. K. Pullen, L. R. Pease: Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989, 77, 51-59.
19. E. Engvall, P. Perlmann: Enzyme-linked immunosorbend assay, ELISA: III. Quantitation of specific antibodies by enzyme-labeled anti-immunglobulin in antigen-coated tubes. J Immunol. 1972, 109, 129-135.
20. T. A. Keating, C. G. Marshall, C. T. Walsh, A. E. Keating: The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains. Nat Struct Biol. 2002, 9, 522-526.
21. A. Lewendon, I. A. Murray, W. V. Shaw, M. R. Gibbs, A. G. Leslie: Replacement of catalytic histidine-195 of chloramphenicol acetyltransferase: Evidence for a general base role for glutamate. Biochemistry. 1994, 33, 1944-1950.