Remarkable alkaline stability of an engineered protein A as immunoglobulin affinity ligand: C domain having only one amino acid substitution

Protein Science

Volumn 22, Issue 9, 2013, Pages 1230-1238

  Minakuchi, Kazunobu ^a   Murata, Dai ^a   Okubo, Yuji ^a   Nakano, Yoshiyuki ^a   Yoshida, Shinichi ^a  

^a KANEKA CORPORATION   (Japan)

Author keywords

Affinity ligand; IgG binding domain; Physicochemical stability; Protein engineering; Therapeutic antibody

Indexed keywords

ARGININE; ASPARAGINE; GLUTAMINE; GLYCINE; IMMUNOGLOBULIN; IMMUNOGLOBULIN G; ISOLEUCINE; LEUCINE; METHIONINE; PHENYLALANINE; PROTEIN C; SERINE; STAPHYLOCOCCUS PROTEIN A; THREONINE; TRYPTOPHAN; TYROSINE; VALINE; ALKALI; LIGAND; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANALYTIC METHOD; ARTICLE; BIACORE ANALYSIS; CHO CELL; CIRCULAR DICHROISM; INCUBATION TIME; LIGAND BINDING; MOLECULAR WEIGHT; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; TANDEM REPEAT; THERMOSTABILITY; CHEMISTRY; GENETICS; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE;

ALKALIES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; GLYCINE; IMMUNOGLOBULINS; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; STAPHYLOCOCCAL PROTEIN A;

EID: 84892946184     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2310     Document Type: Article

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