Selection of reference genes for RT-qPCR analysis in tumor tissues from male hepatocellular carcinoma patients with hepatitis B infection and cirrhosis

Cancer Biomarkers

Volumn 13, Issue 5, 2013, Pages 345-349

  Liu, Shuang ^a   Zhu, Pengfei ^b   Zhang, Ling ^c   Ding, Shanlong ^b   Zheng, Sujun ^a   Wang, Yang ^a   Lu, Fengmin ^b  

^a CAPITAL MEDICAL UNIVERSITY   (China)

^b PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

^c Henan Cancer Hospital   (China)

Author keywords

C terminal binding protein 1; Hepatitis B virus; Hepatocellular carcinoma; Liver cirrhosis; Male gender; Reference gene; RT qPCR

Indexed keywords

BINDING PROTEIN; C TERMINAL BINDING PROTEIN 1; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE 1; PORPHOBILINOGEN DEAMINASE; RNA 18S; UBIQUITIN C; UNCLASSIFIED DRUG;

ARTICLE; CANCER DIAGNOSIS; CANCER PATIENT; CANCER TISSUE; CONTROLLED STUDY; GENE EXPRESSION PROFILING; GENETIC ANALYSIS; HEPATITIS B; HUMAN; HUMAN TISSUE; LIVER CELL CARCINOMA; LIVER CIRRHOSIS; MALE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION; RISK FACTOR;

C-TERMINAL BINDING PROTEIN 1; HEPATITIS B VIRUS; HEPATOCELLULAR CARCINOMA; LIVER CIRRHOSIS; MALE GENDER; REFERENCE GENE; RT-QPCR;

CARCINOMA, HEPATOCELLULAR; GENE EXPRESSION PROFILING; HEPATITIS B, CHRONIC; HUMANS; LIVER CIRRHOSIS; LIVER NEOPLASMS; MALE; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTOME; TUMOR MARKERS, BIOLOGICAL;

EID: 84892911871     PISSN: 15740153     EISSN: 18758592     Source Type: Journal    
DOI: 10.3233/CBM-130365     Document Type: Article

References (25)

1. Heid CA, Stevens J, Livak KJ,Williams PM. Real time quantitative PCR. Genome Res 1996, 6(10). 986-994
2. Gibson UE, Heid CA, Williams PM. A novel method for real time quantitative RT-PCR. Genome Res 1996, 6(10). 995-1001
3. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. Accurate normalization of realtime quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3(7). RESEARCH0034
4. Matsumura M, Ijichi M, Shiratori Y, Togo G, Hikiba Y, Inoued K, Kohara M, Omata M. Simple quantitative assay of alphafetoprotein mRNA in liver tissue using the real-time detection polymerase chain reaction assay - its application for clinical use. Hepatol Res 2001, 20(1). 84-96
5. Sirover MA. New insights into an old protein. The functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta 1999, 1432(2). 159-184
6. Yi M, Schultz DE, Lemon SM. Functional significance of the interaction of hepatitis A virus RNA with glyceraldehyde 3-phosphate dehydrogenase (GAPDH). opposing effects of GAPDH and polypyrimidine tract binding protein on internal ribosome entry site function. J Virol 2000, 74(14). 6459-6468
7. Lin SS, Chang SC, Wang YH, Sun CY, Chang MF. Specific interaction between the hepatitis delta virus RNA and glyceraldehyde 3-phosphate dehydrogenase. an enhancement on ribozyme catalysis. Virology 2000, 271(1). 46-57
8. Vila MR, Nicolas A, Morote J, de I, Meseguer A. Increased glyceraldehyde-3-phosphate dehydrogenase expression in renal cell carcinoma identified by RNA-based, arbitrarily primed polymerase chain reaction. Cancer 2000, 89(1). 152-164
9. Tokunaga K, Nakamura Y, Sakata K, Fujimori K, Ohkubo M, Sawada K, Sakiyama S. Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers. Cancer Res 1987, 47(21). 5616-5619
10. Revillion F, Pawlowski V, Hornez L, Peyrat JP. Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer. Eur J Cancer 2000, 36(8). 1038-1042
11. Harada N, Yasunaga R, Higashimura Y, Yamaji R, Fujimoto K, Moss J, Inui H, Nakano Y. Glyceraldehyde-3-phosphate dehydrogenase enhances transcriptional activity of androgen receptor in prostate cancer cells. J Biol Chem 2007, 282(31). 22651-22661
12. Meyer-Siegler K, Mauro DJ, Seal G, Wurzer J, DeRiel JK, Sirover MA. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc Natl Acad Sci U S A 1991, 88(19). 8460-8464
13. Azam S, Jouvet N, Jilani A, Vongsamphanh R, Yang X, Yang S, Ramotar D. Human glyceraldehyde-3-phosphate dehydrogenase plays a direct role in reactivating oxidized forms of the DNA repair enzyme APE1. J Biol Chem 2008, 283(45). 30632-30641
14. Krynetski EY, Krynetskaia NF, Bianchi ME, Evans WE. A nuclear protein complex containing high mobility group proteins B1 and B2, heat shock cognate protein 70, ERp60, and glyceraldehyde-3-phosphate dehydrogenase is involved in the cytotoxic response to DNA modified by incorporation of anticancer nucleoside analogues. Cancer Res 2003, 63(1). 100-106
15. Gao Q, Wang XY, Fan J, Qiu SJ, Zhou J, Shi YH, Xiao YS, Xu Y, Huang XW, Sun J. Selection of reference genes for realtime PCR in human hepatocellular carcinoma tissues. J Cancer Res Clin Oncol 2008, 134(9). 979-986
16. Ganapathy-Kanniappan S, Kunjithapatham R, Geschwind JF. Glyceraldehyde-3-phosphate dehydrogenase. A promising target for molecular therapy in hepatocellular carcinoma. Oncotarget, 3(9). 940-953
17. Lee MH, Yang HI, Liu J, Batrla-Utermann R, Jen CL, Iloeje UH, Lu SN, You SL, Wang LY, Chen CJ. Prediction models of long-term cirrhosis and HCC risk in chronic hepatitis B patients. Risk scores integrating host and virus profiles. Hepatology
18. Yang HI, Sherman M, Su J, Chen PJ, Liaw YF, Iloeje UH, Chen CJ. Nomograms for risk of hepatocellular carcinoma in patients with chronic hepatitis B virus infection. J Clin Oncol, 28(14). 2437-2444
19. Mittal S, El-Serag HB. Epidemiology of Hepatocellular Carcinoma. Consider the Population. J Clin Gastroenterol
20. Claverie JM. Computational methods for the identification of differential and coordinated gene expression. Human molecular genetics 1999, 8(10). 1821-1832
21. Andersen CL, Jensen JL, Orntoft TF. Normalization of realtime quantitative reverse transcription-PCR data. A modelbased variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 2004, 64(15). 5245-5250
22. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E. Housekeeping genes as internal standards. use and limits. J Biotechnol 1999, 75(2-3). 291-295
23. Gong Y, Cui L, Minuk GY. Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28s-ribosomal RNA gene expression in human hepatocellular carcinoma. Hepatology 1996, 23(4). 734-737
24. Sun W, Xing B, Sun Y, Du X, Lu M, Hao C, Lu Z, Mi W,Wu S,Wei H et al.. Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis. novel protein markers in hepatocellular carcinoma tissues. Mol Cell Proteomics 2007, 6(10). 1798-1808
25. Don Ganem, M.D, and Alfred M. Prince, M.D. Hepatitis B Virus Infection - Natural History and Clinical Consequences. N Engl J Med 2004; 350. 1118-1129