RUSH and CRUSH: A rapid and conditional RNA interference method in mice

Genesis

Volumn 52, Issue 1, 2014, Pages 39-48

  Brown, Juliana R ^a,b,c   Zetsche, Bernd ^a,b   Jackson Grusby, Laurie ^a,b,c  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD STEM CELL INSTITUTE   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

ES cells; Mouse models; RNAi; Transgenesis

Indexed keywords

CRE RECOMBINASE; NESTIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; EMBRYONIC STEM CELL; EXPRESSION VECTOR; FLUORESCENCE ANALYSIS; GENE EXPRESSION SYSTEM; GENE FUNCTION; GENE MUTATION; GENE SILENCING; MOUSE; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; TETRAPLOIDY; TISSUE SPECIFICITY; TRANSGENIC MOUSE;

MUS; MUS MUSCULUS;

ES CELLS; MOUSE MODELS; RNAI; TRANSGENESIS;

ANIMALS; CELL LINE; EMBRYONIC STEM CELLS; GENE KNOCKDOWN TECHNIQUES; GENETIC VECTORS; HEK293 CELLS; HUMANS; MICE; MICE, TRANSGENIC; MODELS, ANIMAL; REPRODUCIBILITY OF RESULTS; RNA INTERFERENCE;

EID: 84892880783     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.22718     Document Type: Article

References (30)

1. Altschul SF, Gish W. 1996. Local alignment statistics. Methods Enzymol 266:460-480.
2. Dickins RA, Mcjunkin K, Hernando E, Premsrirut PK, Krizhanovsky V, Burgess DJ, Kim SY, Cordon-Cardo C, Zender L, Hannon GJ, Lowe SW. 2007. Tissue-specific and reversible RNA interference in transgenic mice. Nat Genet 39:914-921.
3. Eggan K, Akutsu H, Loring J, Jackson-Grusby L, Klemm M, Rideout WM, Yanagimachi R, Jaenisch R. 2001. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc Natl Acad Sci USA 98:6209-6214.
4. Ehlert EM, Eggers R, Niclou SP, Verhaagen J. 2010. Cellular toxicity following application of adeno-associated viral vector-mediated RNA interference in the nervous system. BMC Neurosci 11:20.
5. Grace MB, McLeland CB, Blakely WF. 2002. Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry. Int J Radiat Biol 78:1011-1021.
6. Grimm D, Streetz KL, Jopling CL, Storm TA, Pandey K, Davis CR, Marion P, Salazar F, Kay MA. 2006. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 441:537-541.
7. Hannon GJ, Rossi JJ. 2004. Unlocking the potential of the human genome with RNA interference. Nature 431:371-378.
8. Hitz C, Wurst W, Kühn R. 2007. Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference. Nucleic Acids Res 35:e90.
9. Ho Y, Wigglesworth K, Eppig JJ, Schultz RM. 1995. Preimplantation development of mouse embryos in KSOM: Augmentation by amino acids and analysis of gene expression. Mol Reprod Dev 41:232-238.
10. Jackson AL, Linsley PS. 2010. Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application. Nat Rev Drug Discov 9:57-67.
11. Khan AA, Betel D, Miller ML, Sander C, Leslie CS, Marks DS. 2009. Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs. Nat Biotechnol 27:549-555.
12. Kunath T, Gish G, Lickert H, Jones N, Pawson T, Rossant J. 2003. Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype. Nat Biotechnol 21:559-561.
13. Laugwitz KL, Moretti A, Caron L, Nakano A, Chien KR. 2008. Islet1 cardiovascular progenitors: A single source for heart lineages? Development 135:193-205.
14. Lee A, Kessler JD, Read T-A, Kaiser C, Corbeil D, Huttner WB, Johnson JE, Wechsler-Reya RJ. 2005. Isolation of neural stem cells from the postnatal cerebellum. Nat Neurosci 8:723-729.
15. Liu YP, Berkhout B. 2011. miRNA cassettes in viral vectors: Problems and solutions. Biochem Biophys Acta 1809:732-745.
16. McBride JL, Boudreau RL, Harper SQ, Staber PD, Monteys AM, Martins I, Gilmore BL, Burstein H, Peluso RW, Polisky B, Carter BJ, Davidson BL. 2008. Artificial miRNAs mitigate shRNA-mediated toxicity in the brain: Implications for the therapeutic development of RNAi. Proc Natl Acad Sci USA 105:5868-5873.
17. Molofsky AV, Pardal R, Iwashita T, Park I-K, Clarke MF, Morrison SJ. 2003. Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation. Nature 425:962-967.
18. Nagy A, Gocza E, Diaz EM, Prideaux VR, Ivanyi E, Markkula M, Rossant J. 1990. Embryonic stem cells alone are able to support fetal development in the mouse. Development 110:815-821.
19. Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, Hannon GJ, Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145:145-158.
20. Rajewsky K, Gu H, Kuhn R, Betz UA, Muller W, Roes J, Schwenk F. 1996. Conditional gene targeting. J Clin Invest 98:600-603.
21. Ryder E, Gleeson D, Sethi D, Vyas S, Miklejewska E, Dalvi P, Habib B, Cook R, Hardy M, Jhaveri K, Bottomley J, Wardle-Jones H, Bussell JN, Houghton R, Salisbury J, Skarnes WC, Sanger Mouse Genetics P, Ramirez-Solis R. 2013. Molecular Characterization of Mutant Mouse Strains Generated from the EUCOMM/KOMP-CSD ES Cell Resource. Mamm Genome 24:286-294.
22. Schönig K, Schwenk F, Rajewsky K, Bujard H. 2002. Stringent doxycycline dependent control of CRE recombinase in vivo. Nucleic Acids Res 30:e134.
23. Seibler J, Schwenk F. 2010. Transgenic RNAi applications in the mouse. Methods Enzymol 477:367-386.
24. Soriano P. 1999. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21:70-71.
25. Tronche F, Kellendonk C, Kretz O, Gass P, Anlag K, Orban PC, Bock R, Klein R, Schütz G. 1999. Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety. Nat Genet 23:99-103.
26. Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T. 2004. Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci USA 101:10380-10385.
27. Vidigal JA, Morkel M, Wittler L, Brouwer-Lehmitz A, Grote P, Macura K, Herrmann BG. 2010. An inducible RNA interference system for the functional dissection of mouse embryogenesis. Nucleic Acids Res 38:e122.
28. Wutz A, Rasmussen TP, Jaenisch R. 2002. Chromosomal silencing and localization are mediated by different domains of Xist RNA. Nat Genet 30:167-174.
29. Yang L, Cai CL, Lin L, Qyang Y, Chung C, Monteiro RM, Mummery CL, Fishman GI, Cogen A, Evans S. 2006. Isl1Cre reveals a common Bmp pathway in heart and limb development. Development 133:1575-1585.
30. Yu J, McMahon AP. 2006. Reproducible and inducible knockdown of gene expression in mice. Genesis 44:252-261.