Cancer-associated fibroblasts enhance the migration ability of ovarian cancer cells by increasing EZH2 expression

International Journal of Molecular Medicine

Volumn 33, Issue 1, 2014, Pages 91-96

  Xu, Linjuan ^a   Deng, Qingchun ^a,b   Pan, Yinglian ^b   Peng, Minggang ^a   Wang, Xiaoling ^a   Song, Limian ^a   Xiao, Man ^a   Wang, Zehua ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Cancer associated fibroblasts; Enhancer of zeste homologue 2; Migration; Ovarian cancer

Indexed keywords

BETA ACTIN; SHORT HAIRPIN RNA; TRANSCRIPTION FACTOR EZH2;

ARTICLE; CANCER ASSOCIATED FIBROBLAST; CANCER CELL CULTURE; CELL MIGRATION; CELL MIGRATION ASSAY; COCULTURE; CONTROLLED STUDY; EPIGENETICS; FEMALE; FIBROBLAST; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MALIGNANT TRANSFORMATION; OVARY CANCER; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR GROWTH; TUMOR MICROENVIRONMENT; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE, TUMOR; CELL MOVEMENT; COCULTURE TECHNIQUES; FEMALE; FIBROBLASTS; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; OVARIAN NEOPLASMS; POLYCOMB REPRESSIVE COMPLEX 2; REAL-TIME POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; TUMOR MICROENVIRONMENT;

EID: 84892751918     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2013.1549     Document Type: Article

References (25)

1. Sarojini S, Tamir A, Lim H, et al: Early detection biomarkers for ovarian cancer. J Oncol 2012: 709049, 2012.
2. Bijron JG, Bol GM, Verheijen RH and van Diest PJ: Epigenetic biomarkers in the diagnosis of ovarian cancer. Expert Opin Med Diagn 6: 421-438, 2012.
3. Naora H and Montell DJ: Ovarian cancer metastasis: integrating insights from disparate model organisms. Nat Rev Cancer 5: 355-366, 2005.
4. Chou J and Werb Z: MicroRNAs play a big role in regulating ovarian cancer-associated fibroblasts and the tumor microenvironment. Cancer Discov 2: 1078-1080, 2012.
5. Mitra AK, Zillhardt M, Hua Y, et al: MicroRNAs reprogram normal fibroblasts into cancer-associated fibroblasts in ovarian cancer. Cancer Discov 2: 1100-1108, 2012.
6. Finak G, Bertos N, Pepin F, et al: Stromal gene expression predicts clinical outcome in breast cancer. Nat Med 14: 518-527, 2008.
7. Wintzell M, Hjerpe E, Avall Lundqvist E and Shoshan M: Protein markers of cancer-associated fibroblasts and tumor-initiating cells reveal subpopulations in freshly isolated ovarian cancer ascites. BMC Cancer 12: 359, 2012.
8. Ko SY, Barengo N, Ladanyi A, et al: HOXA9 promotes ovarian cancer growth by stimulating cancer-associated fibroblasts. J Clin Invest 122: 3603-3617, 2012.
9. Schauer IG, Sood AK, Mok S and Liu J: Cancer-associated fibroblasts and their putative role in potentiating the initiation and development of epithelial ovarian cancer. Neoplasia 13: 393-405, 2011.
10. Zhang Y, Tang H, Cai J, et al: Ovarian cancer-associated fibroblasts contribute to epithelial ovarian carcinoma metastasis by promoting angiogenesis, lymphangiogenesis and tumor cell invasion. Cancer Lett 303: 47-55, 2011.
11. Cai J, Tang H, Xu L, et al: Fibroblasts in omentum activated by tumor cells promote ovarian cancer growth, adhesion and invasiveness. Carcinogenesis 33: 20-29, 2012.
12. Cao R, Wang L, Wang H, et al: Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science 298: 1039-1043, 2002.
13. Kirmizis A, Bartley SM, Kuzmichev A, et al: Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27. Genes Dev 18: 1592-1605, 2004.
14. Simon JA and Lange CA: Roles of the EZH2 histone methyltransferase in cancer epigenetics. Mutat Res 647: 21-29, 2008.
15. Hu S, Yu L, Li Z, et al: Overexpression of EZH2 contributes to acquired cisplatin resistance in ovarian cancer cells in vitro and in vivo. Cancer Biol Ther 10: 788-795, 2010.
16. Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408, 2001.
17. Sehouli J, Loddenkemper C, Cornu T, et al: Epigenetic quantification of tumor-infiltrating T-lymphocytes. Epigenetics 6: 236-246, 2011.
18. Shahrzad S, Bertrand K, Minhas K and Coomber BL: Induction of DNA hypomethylation by tumor hypoxia. Epigenetics 2: 119-125, 2007.
19. Kalluri R and Zeisberg M: Fibroblasts in cancer. Nat Rev Cancer 6: 392-401, 2006.
20. Shimoda M, Mellody KT and Orimo A: Carcinoma-associated fibroblasts are a rate-limiting determinant for tumour progression. Semin Cell Dev Biol 21: 19-25, 2010.
21. Xing F, Saidou J and Watabe K: Cancer associated fibroblasts (CAFs) in tumor microenvironment. Front Biosci (Landmark Ed) 15: 166-179, 2010.
22. Li H, Cai Q, Godwin AK and Zhang R: Enhancer of zeste homolog 2 promotes the proliferation and invasion of epithelial ovarian cancer cells. Mol Cancer Res 8: 1610-1618, 2010.
23. Matsumoto K and Nakamura T: Hepatocyte growth factor and the Met system as a mediator of tumor-stromal interactions. Int J Cancer 119: 477-483, 2006.
24. Orimo A, Gupta PB, Sgroi DC, et al: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Cell 121: 335-348, 2005.
25. Lu C, Han HD, Mangala LS, et al: Regulation of tumor angiogenesis by EZH2. Cancer Cell 18: 185-197, 2010.