β-alanine suppresses malignant breast epithelial cell aggressiveness through alterations in metabolism and cellular acidity in vitro

Molecular Cancer

Volumn 13, Issue 1, 2014, Pages

  Vaughan, Roger A ^a,b   Gannon, Nicholas P ^b   Garcia Smith, Randi ^b   Licon Munoz, Yamhilette ^b   Barberena, Miguel A ^b   Bisoffi, Marco ^b,c,d   Trujillo, Kristina A ^b,c  

^a UNIVERSITY OF NEW MEXICO   (United States)

^b UNIVERSITY OF NEW MEXICO HEALTH SCIENCES CENTER   (United States)

^c UNIVERSITY OF NEW MEXICO   (United States)

^d CHAPMAN UNIVERSITY   (United States)

Author keywords

Carnosine; Doxorubicin; Glycolysis; Migration; Mitochondria; Oxidative metabolism; Proliferation; Warburg metabolism

Indexed keywords

BETA-ALANINE; BLOTTING, WESTERN; BREAST NEOPLASMS; FLOW CYTOMETRY; GLYCOLYSIS; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; MCF-7 CELLS; MICROSCOPY, CONFOCAL; MITOCHONDRIA; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84892725746     PISSN: None     EISSN: 14764598     Source Type: Journal    
DOI: 10.1186/1476-4598-13-14     Document Type: Article

References (27)

1. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell 2011, 144:646-674. 10.1016/j.cell.2011.02.013, 21376230.
2. Sotgia F, Whitaker-Menezes D, Martinez-Outschoorn UE, Flomenberg N, Birbe RC, Witkiewicz AK, Howell A, Philp NJ, Pestell RG, Lisanti MP. Mitochondrial metabolism in cancer metastasis Visualizing tumor cell mitochondria and the " reverse Warburg effect" in positive lymph node tissue. Cell Cycle 2012, 11:1445-1454. 10.4161/cc.19841, 3350881, 22395432.
3. Witkiewicz AK, Whitaker-Menezes D, Dasgupta A, Philp NJ, Lin Z, Gandara R, Sneddon S, Martinez-Outschoorn UE, Sotgia F, Lisanti MP. Using the " reverse Warburg effect" to identify high-risk breast cancer patients Stromal MCT4 predicts poor clinical outcome in triple-negative breast cancers. Cell Cycle 2012, 11:1108-1117. 10.4161/cc.11.6.19530, 3335917, 22313602.
4. Gaunitz F, Hipkiss AR. Carnosine and cancer: a perspective. Amino Acids 2012, 43:135-142. 10.1007/s00726-012-1271-5, 22454085.
5. Young CD, Anderson SM. Sugar and fat - that's where it's at: metabolic changes in tumors. Breast Cancer Research 2008, 10:202. 10.1186/bcr1852, 2374962, 18304378.
6. Gatenby RA, Gillies RJ. Why do cancers have high aerobic glycolysis?. Nat Rev Cancer 2004, 4:891-899. 10.1038/nrc1478, 15516961.
7. Simon SM, Schindler M. Cell biological mechanisms of multidrug-resistance in tumors. Proc Natl Acad Sci U S A 1994, 91:3497-3504. 10.1073/pnas.91.9.3497, 43607, 7909602.
8. Raghunand N, He X, van Sluis R, Mahoney B, Baggett B, Taylor CW, Paine-Murrieta G, Roe D, Bhujwalla ZM, Gillies RJ. Enhancement of chemotherapy by manipulation of tumour pH. Br J Cancer 1999, 80:1005-1011. 10.1038/sj.bjc.6690455, 2363059, 10362108.
9. Caruso J, Charles J, Unruh K, Giebel R, Learmonth L, Potter W. Ergogenic effects of beta-alanine and carnosine: proposed future research to quantify their efficacy. Nutrients 2012, 4:585-601. 10.3390/nu4070585, 3407982, 22852051.
10. Jordan T, Lukaszuk J, Misic M, Umoren J. Effect of beta-alanine supplementation on the onset of blood lactate accumulation (OBLA) during treadmill running: pre/post 2 treatment experimental design. J Int Soc Sports Nutr 2010, 7:20. 10.1186/1550-2783-7-20, 2887393, 20482881.
11. Asperger A, Renner C, Menzel M, Gebhardt R, Meixensberger J, Gaunitz F. Identification of factors involved in the anti-tumor activity of carnosine on glioblastomas using a proteomics approach. Cancer Invest 2011, 29:272-281. 10.3109/07357907.2010.550666, 21345069.
12. Renner C, Seyffarth A, de Arriba SG, Meixensberger J, Gebhardt R, Gaunitz F. Carnosine inhibits growth of cells isolated from human glioblastoma multiforme. Int J Pept Res Ther 2008, 14:127-135.
13. Renner C, Zemitzsch N, Fuchs B, Geiger KD, Hermes M, Hengstler J, Gebhardt R, Meixensberger J, Gaunitz F. Carnosine retards tumor growth in vivo in an NIH3T3-HER2/neu mouse model. Mol Cancer 2010, 9:2. 10.1186/1476-4598-9-2, 2818694, 20053283.
14. Renner C, Asperger A, Seyffarth A, Meixensberger J, Gebhardt R, Gaunitz F. Carnosine inhibits ATP production in cells from malignant glioma. Neurol Res 2010, 32:101-105.
15. Holliday R, McFarland GA. Inhibition of the growth of transformed and neoplastic cells by the dipeptide carnosine. Br J Cancer 1996, 73:966-971. 10.1038/bjc.1996.189, 2075811, 8611433.
16. Wikstrom JD, Sereda SB, Stiles L, Elorza A, Allister EM, Neilson A, Ferrick DA, Wheeler MB, Shirihai OS. A novel high-throughput assay for islet respiration reveals uncoupling of rodent and human islets. Plos One 2012, 7:5.
17. Giulivi C, Ross-Inta C, Horton AA, Luckhart S. Metabolic pathways in Anopheles stephensi mitochondria. Biochem J 2008, 415:309-316. 10.1042/BJ20080973, 18588503.
18. Vaughan RA, Garcia-Smith R, Dorsey J, Griffith JK, Bisoffi M, Trujillo KA. Tumor necrosis factor alpha induced warburg-like metabolism and is reversed by anti-inflammatory curcumin in breast epithelial cells. Int J Cancer 2013, 133:2504-2510. 10.1002/ijc.28264, 23661584.
19. Vaughan RA, Garcia-Smith R, Trujillo KA, Bisoffi M. Tumor necrosis factor alpha increases aerobic glycolysis and reduces oxidative metabolism in prostate epithelial cells. The Prostate 2013, 73:1538-1546. 10.1002/pros.22703, 23818177.
20. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001, 29:e25. 10.1093/nar/29.4.e25, 29632, 11160945.
21. Trujillo KA, Heaphy CM, Mai M, Vargas KM, Jones AC, Phung V, Butler KS, Joste NE, Bisoffi M, Griffith JK. Markers of fibrosis and epithelial to mesenchymal transition demonstrate field cancerization in histologically normal tissue adjacent to breast tumors. Int J Cancer 2011, 129:1310-1321. 10.1002/ijc.25788, 3249233, 21105047.
22. Trujillo KA, Hines WC, Vargas KM, Jones AC, Joste NE, Bisoffi M, Griffith JK. Breast field cancerization: isolation and comparison of telomerase-expressing cells in tumor and tumor adjacent, histologically normal breast tissue. Mol Cancer Res 2011, 9:1209-1221. 10.1158/1541-7786.MCR-10-0424, 3249616, 21775421.
23. Wu G, Lu J, Gua J, Huang M, Gan L, Chen X, Wang Y. Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells. Pharmacol Rep 2013, 65:453-459.
24. Baguet A, Koppo K, Pottier A, Derave W. Beta-Alanine supplementation reduces acidosis but not oxygen uptake response during high-intensity cycling exercise. Eur J Appl Physiol 2010, 108:495-503. 10.1007/s00421-009-1225-0, 19841932.
25. Rofstad EK, Mathiesen B, Kindem K, Galappathi K. Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice. Cancer Res 2006, 66:6699-6707. 10.1158/0008-5472.CAN-06-0983, 16818644.
26. Kato Y, Lambert CA, Colige AC, Mineur P, Noel A, Frankenne F, Foidart JM, Baba M, Hata R, Miyazaki K, Tsukuda M. Acidic extracellular pH induces matrix metalloproteinase-9 expression in mouse metastatic melanoma cells through the phospholipase D-mitogen-activated protein kinase signaling. J Biol Chem 2005, 280:10938-10944. 10.1074/jbc.M411313200, 15657063.
27. Harris RC, Tallon MJ, Dunnett M, Boobis L, Coakley J, Kim HJ, Fallowfield JL, Hill CA, Sale C, Wise JA. The absorption of orally supplied beta-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino Acids 2006, 30:279-289. 10.1007/s00726-006-0299-9, 16554972.