Transcript abundance of phytoene synthase 1 and phytoene synthase 2 is associated with natural variation of storage root carotenoid pigmentation in carrot

Journal of the American Society for Horticultural Science

Volumn 139, Issue 1, 2014, Pages 63-68

  Bowman, Megan J ^a,b   Willis, David K ^a   Simon, Philipp W ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Daucus carota; Gene expression; Naturally occurring mutations; RT qPCR

Indexed keywords

EID: 84892568596     PISSN: 00031062     EISSN: None     Source Type: Journal    
DOI: 10.21273/jashs.139.1.63     Document Type: Article

References (35)

1. Auldridge, M., D.R. McCarty, and H.J. Klee. 2006. Plant carotenoid cleavage oxygenases and their apocarotenoid products. Curr. Opin. Plant Biol. 9:315-321.
2. Ben-Shaul, Y. and S. Klein. 1965. Development and structure of carotene bodies in carrot roots. Bot. Gaz. 126:79-85.
3. Buckner, B., P. Miguel, D. Janick-Buckner, and J. Bennetzen. 1996. The Y1 gene of maize codes for phytoene synthase. Genetics 143:479-488.
4. Bustin, S.A., V. Benes, J.A. Garson, J. Hellemans, J. Huggett, M. Kubista, R. Mueller, T. Nolan, M.W. Pfaffl, G.L. Shipley, J. Vandespompele, and C.T. Wittwer. 2009. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55:611-622.
5. Cazzonelli, C.I. 2011. Carotenoids in nature: Insights from plants and beyond. Funct. Plant Biol. 38:833-847.
6. Cazzonelli, C.I. and B.J. Pogson. 2010. Source to sink: Regulation of carotenoid biosynthesis in plants. Trends Plant Sci. 15:266-274.
7. Clotault, J., D. Peltier, R. Berruyer, M. Thomas, M. Briard, and E. Geoffriau. 2008. Expression of carotenoid biosynthesis genes during carrot root development. J. Expt. Bot. 59:3563-3573.
8. Fray, R. and D. Grierson. 1993. Identification and genetic-analysis of normal and mutant phytoene synthase genes of tomato by sequencing, complementation and co-suppression. Plant Mol. Biol. 22:589-602.
9. Harjes, C.E., T.R. Rocheford, L. Bai, T.P. Brutnell, C.B. Kandianis, S.G. Sowinski, A.E. Stapleton, R. Vallabhaneni, M. Williams, E.T. Wurtzel, J. Yan, and E.S. Buckler. 2008. Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification. Science 319:330-333.
10. Huh, J.H., B.C. Kang, S.H. Nahm, S. Kim, K.S. Ha, M.H. Lee, and B.D. Kim. 2001. A candidate gene approach identified phytoene synthase as the locus for mature fruit color in red pepper (Capsicum spp.). Theor. Appl. Genet. 102:524-530.
11. Isaacson, T., G. Ronen, D. Zamir, and J. Hirschberg. 2002. Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of b-carotene and xanthophylls in plants. Plant Cell 14:333-342.
12. Just, B.J. 2004. Genetic mapping of carotenoid pathway structural genes and major gene QTLs for carotenoid accumulation in wild and domesticated carrot (Daucus carota L.). PhD diss., Univ. Wisconsin, Madison, WI. Didd. Abstr. AAT 3155154.
13. Just, B.J., C.A.F. Santos, M.E.N. Fonseca, L.S. Boiteux, B.B. Oloizia, and P.W. Simon. 2007. Carotenoid biosynthesis structural genes in carrot (Daucus carota): Isolation, sequence characterization, single nucleotide polymorphism (SNP) markers and genome mapping. Theor. Appl. Genet. 114:693-704.
14. Just, B.J., C.A.F. Santos, B.S. Yandell, and P.W. Simon. 2009. Major QTL for carrot color are positionally associated with carotenoid biosynthetic genes and interact epistatically in a wild·domesticated carrot cross. Theor. Appl. Genet. 119:1155-1169.
15. Khachik, F., G.R. Beecher, M.B. Goli, and W. Lusby. 1992. Separation and quantification of carotenoids in foods. Methods Enzymol. 213:347-359.
16. Li, L. and D. Garvin. 2003. Molecular mapping of or, a gene inducing beta-carotene accumulation in cauliflower (Brassica oleracea L. var. botrytis). Genome 46:588-594.
17. Li, L., S. Lu, K.M. Cosman, E.D. Earle, D.F. Garvin, and J. O'Neill. 2006. Beta-carotene accumulation induced by the cauliflower or gene is not due to an increased capacity of biosynthesis. Phytochemistry 67:1177-1184.
18. Li, L., S. Lu, D. O'Halloran, D. Garvin, and J. Vrebalov. 2003. Highresolution genetic and physical mapping of the cauliflower highbeta-carotene gene or (orange). Mol. Genet. Genomics 270:132-138.
19. Li, L., D. Paolillo, M. Parthasarathy, E. DiMuzio, and D. Garvin. 2001. A novel gene mutation that confers abnormal patterns of betacarotene accumulation in cauliflower (Brassica oleracea var. botrytis). Plant J. 26:59-67.
20. Li, L. and J. Van Eck. 2007. Metabolic engineering of carotenoid accumulation by creating a metabolic sink. Transgenic Res. 16:581-585.
21. Lu, S., J. Van Eck, X. Zhou, A.B. Lopez, D.M. O'Halloran, K.M. Cosman, B. Conlin, D.J. Paolillo, D.F. Garvin, J. Vrebalov, L.V. Kochian, H. Kupper, E. Earle, J. Cao, and L. Li. 2006. The cauliflower or gene encodes a DnaJ cysteine-rich domain-containing protein that mediates high levels of beta-carotene accumulation. Plant Cell 18:3594-3605.
22. Maass, D., J. Arango, F. Wuest, P. Beyer, and R. Welsch. 2009. Carotenoid crystal formation in Arabidopsis and carrot roots caused by increased phytoene synthase protein levels. PLoS One 4:e6373.
23. Moehs, C.P., L. Tian, K. Osteryoung, and D. DellaPenna. 2001. Analysis of carotenoid biosynthetic gene expression during marigold petal development. Plant Mol. Biol. 45:281-293.
24. Okada, K., H. Kasahara, S. Yamaguchi, H. Kawaide, Y. Kamiya, H. Nojiri, and H. Yamane. 2008. Genetic evidence for the role of isopentenyl diphosphate isomerases in the mevalonate pathway and plant development in Arabidopsis. Plant Cell Physiol. 49:604-616.
25. Pfaffl, M. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45.
26. Pfaffl, M., A. Tichopad, C. Prgomet, and T. Neuvians. 2004. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26:509-515.
27. Rasmussen, R. 2001. Quantification on the LightCycler instrument, p. 31-34. In: Meurer, S., C. Wittwer, and K. Nakagawara (eds.). Rapid cycle real-time PCR: Methods and applications. Springer-Verlag, Heidelberg, Germany.
28. R Development Core Team. 2011. R: A language and environment for statistical computing. 13 Sept. 2012. .
29. Ronen, G., L. Carmel-Goren, D. Zamir, and J. Hirschberg. 2000. An alternative pathway to beta-carotene formation in plant chromoplasts discovered by map-based cloning of beta and old gold color mutations in tomato. Proc. Natl. Acad. Sci. USA 97:11102-11107.
30. Santos, C., D. Senalik, and P. Simon. 2005. Path analysis suggests phytoene accumulation is the key step limiting the carotenoid pathway in white carrot roots. Genet. Mol. Biol. 28:287-293.
31. Simon, P.W., L.M. Pollak, B.A. Clevidence, J.M. Holden, and D.B. Haytowitz. 2009. Plant breeding for human nutritional quality. Plant Breed. Rev. 31:325-415.
32. Simon, P.W. and X.Y. Wolff. 1987. Carotenes in typical and dark orange carrots. J. Agr. Food Chem. 35:1017-1022.
33. Thorup, T.A., B. Tanyolac, K.D. Livingstone, S. Popovsky, I. Paran, and M. Jahn. 2000. Candidate gene analysis of organ pigmentation loci in the Solanaceae. Proc. Natl. Acad. Sci. USA 97:11192-11197.
34. Vallabhaneni, R., L.M.T. Bradbury, and E.T. Wurtzel. 2010. The carotenoid dioxygenase gene family in maize, sorghum, and rice. Arch. Biochem. Biophys. 504:104-111.
35. Vallabhaneni, R. and E.T. Wurtzel. 2009. Timing and biosynthetic potential for carotenoid accumulation in genetically diverse germplasm of maize. Plant Physiol. 150:562-572.