Blind unmixing of spectrally resolved lifetime images

Journal of Biomedical Optics

Volumn 18, Issue 8, 2013, Pages

  Fereidouni, Farzad ^a   Blab, Gerhard A ^a   Gerritsen, Hans C ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

fluorescence lifetime imaging; fluorescence microscopy; image analysis; spectral imaging; spectral unmixing

Indexed keywords

COMBINED ANALYSIS; FLUORESCENCE LIFETIME IMAGING; FLUORESCENT DYES; INDIVIDUAL COMPONENTS; RELIABILITY AND SENSITIVITY; RESOLVED FLUORESCENCE; SPECTRAL IMAGING; SPECTRAL UNMIXING;

FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; SPECTROSCOPY;

FLUORESCENCE;

ALGORITHM; ARTICLE; AUTOMATED PATTERN RECOGNITION; COMPUTER ASSISTED DIAGNOSIS; FLUORESCENCE MICROSCOPY; IMAGE ENHANCEMENT; IMAGE SUBTRACTION; METHODOLOGY; MOLECULAR IMAGING; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY; PROCEDURES;

ALGORITHMS; IMAGE ENHANCEMENT; IMAGE INTERPRETATION, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; PATTERN RECOGNITION, AUTOMATED; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; SUBTRACTION TECHNIQUE;

EID: 84892490015     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.JBO.18.8.086006     Document Type: Article

References (19)

1. T. Zimmermann, "Spectral imaging and linear unmixing in light microscopy," Adv Biochem Engin/Biotechnol. 95, 245-265 (2005). (Pubitemid 44617143)
2. F. Fereidouni, A. N. Bader, and H. C. Gerritsen, "Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images," Opt. Express 20(12), 12729-12741 (2012).
3. R. A. Neher et al., "Blind source separation techniques for the decomposition of multiply labeled fluorescence images," Biophys. J. 96(9), 3791-3800 (2009).
4. I. Jolliffe, Principal Component Analysis, Spinger-Verlag, Spinger-Verlag(2002).
5. D. Chorvat, Jr. et al., "Spectral unmixing of flavin autofluorescence components in cardiac myocytes," Biophys. J. 89(6), L55 (2005).
6. P. Pande, B. E. Applegate, and J. A. Jo, "Application of non-negative matrix factorization to multispectral FLIM data analysis," Biomed. Opt. Express 3(9), 2244-2262 (2012).
7. H. Shirakawa and S. Miyazaki, "Blind spectral decomposition of single-cell fluorescence by parallel factor analysis," Biophys. J. 86(3), 1739-1752 (2004). (Pubitemid 38295608)
8. S. Schlachter et al., "A method to unmix multiple fluorophores in microscopy images with minimal a priori information," Opt. Express 17(25), 22747-22760 (2009).
9. H. D. Vishwasrao et al., "Conformational dependence of intracellular NADH on metabolic state revealed by associated fluorescence anisotropy," J. Biol. Chem. 280(26), 25119-25126 (2005).
10. J. A. Palero et al., "In vivo monitoring of protein-bound and free NADH during ischemia by nonlinear spectral imaging microscopy," Biomed. Opt. Express 2(5), 1030-1039 (2011).
11. Q. Hanley and A. Clayton, "AB-plot assisted determination of fluorophore mixtures in a fluorescence lifetime microscope using spectra or quenchers," J. Microsc. 218(1), 62-67 (2005). (Pubitemid 40524169)
12. Y. C. Chen and R. Clegg, "Spectral resolution in conjunction with polar plots improves the accuracy and reliability of FLIM measurements and estimates of FRET efficiency," J. Microsc. 244(1), 21-37 (2011).
13. F. Fereidouni et al., "A modified phasor approach for analyzing timegated fluorescence lifetime images," J. Microsc. 244(3), 248-258 (2011).
14. F. Fereidouni, K. Reitsma, and H. Gerritsen, "High speed multispectral fluorescence lifetime imaging," Opt. Express 21(10), 11782-11782 (2013).
15. J. A. Palero and H. S. De Bruijn, "Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues," Biophys. J. 93(3), 992 (2007).
16. S. Schlachter et al., "mhFLIM: resolution of heterogeneous fluorescence decays in widefield lifetime microscopy," Opt. Express 17(3), 1557-1570 (2009).
17. F. Bestvater et al., "Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging," J. Microsc. 208(2), 108-115 (2002). (Pubitemid 35356983)
18. T. W. Gadella, FRET and FLIM Techniques, Elsevier Science, Amsterdam, Netherlands (2008).
19. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer, New York (2006).