An Optimized Protocol to Increase Virus-Induced Gene Silencing Efficiency and Minimize Viral Symptoms in Petunia

Plant Molecular Biology Reporter

Volumn 32, Issue 1, 2014, Pages 219-233

  Broderick, Shaun R ^a   Jones, Michelle L ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Chalcone synthase; Green fluorescent protein; Necrosis; Phytoene desaturase; Solanaceae; Tobacco rattle virus

Indexed keywords

NICOTIANA TABACUM; PETUNIA; SOLANACEAE; TOBACCO RATTLE VIRUS;

EID: 84892480462     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-013-0647-3     Document Type: Article

References (50)

1. Al-Kaff NS, Covey SN, Kreike MM, Page AM, Pinder R, Dale PJ (1998) Transcriptional and posttranscriptional plant gene silencing in response to a pathogen. Science 279(5359): 2113-2115.
2. Benedito VA, Visser PB, Angenent GC, Krens FA (2004) The potential of virus-induced gene silencing for speeding up functional characterization of plant genes. Genet Mol Res 3(3): 323-341.
3. Brigneti G, Martín-Hernández AM, Jin H et al (2004) Virus-induced gene silencing in Solanum species. Plant J 39(2): 264-272.
4. Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP (2004) Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J 39(5): 734-746.
5. Burch-Smith TM, Schiff M, Liu Y, Dinesh-Kumar SP (2006) Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol 142(1): 21-27.
6. Bustin SA, Benes V, Garson JA et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55(4): 611-622.
7. Caplan J, Dinesh-Kumar SP (2006) Using viral vectors to silence endogenous genes. Curr Protoc Microbiol I61: 6. 1-6. 13. doi: 10. 1002/9780471729259. mc16i06s01.
8. Chapin LJ, Jones ML (2009) Ethylene regulates phosphorus remobilization and expression of a phosphate transporter (PhPT1) during petunia corolla senescence. J Exp Bot 60(7): 2179-2190.
9. Chen J, Jiang C, Gookin T, Hunter D, Clark D, Reid M (2004) Chalcone synthase as a reporter in virus-induced gene silencing studies of flower senescence. Plant Mol Biol 55(4): 521-530.
10. Chen J, Jiang C, Reid MS (2005) Silencing a prohibitin alters plant development and senescence. Plant J 44(1): 16-24.
11. Dalmay T, Hamilton A, Mueller E, Baulcombe DC (2000) Potato Virus X amplicons in Arabidopsis mediate genetic and epigenetic gene silencing. Plant Cell 12(3): 369-379.
12. Deng X, Elomaa P, Nguyen CX, Hytönen T, Valkonen J, Teeri TH (2012) Virus-induced gene silencing for Asteraceae-a reverse genetics approach for functional genomics in Gerbera hybrida. Plant Biotechnol J 10(8): 970-978.
13. Dillen W, De Clercq J, Kapila J, Zambre M, van Montagu M, Angenon G (1997) The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants. Plant J 12(6): 1459-1463.
14. Donaire L, Barajas D, Martínez-García B, Martínez-Priego L, Pagán I, Llave C (2008) Structural and genetic requirements for the biogenesis of Tobacco Rattle Virus-derived small interfering RNAs. J Virol 82(11): 5167-5177.
15. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391(6669): 806-811.
16. Gerats T, Vandenbussche M (2005) A model system comparative for research: Petunia. Trends Plant Sci 10(5): 251-256.
17. Ghazala W, Varrelmann M (2007) Tobacco rattle virus 29K movement protein is the elicitor of extreme and hypersensitive-like resistance in two cultivars of Solanum tuberosum. Mol Plant Microbe In 20(11): 1396-1405.
18. Gould B, Kramer EM (2007) Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae). Plant Methods 3(1): 6.
19. Grünewald K, Eckert M, Hirschberg J, Hagen C (2000) Phytoene desaturase is localized exclusively in the chloroplast and up-regulated at the mRNA level during accumulation of secondary carotenoids in Haematococcus pluvialis (Volvocales, Chlorophyceae). Plant Physiol 122(4): 1261-1268.
20. Hartl M, Merker H, Schmidt DD, Baldwin IT (2008) Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls. New Phytol 179(2): 356-365.
21. Hernandez-Garcia CM, Bouchard RA, Rushton PJ et al (2010) High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline. BMC Plant Biol 10: 237.
22. Hileman LC, Drea S, Martino G, Litt A, Irish VF (2005) Virus-induced gene silencing is an effective tool for assaying gene function in the basal eudicot species Papaver somniferum (opium poppy). Plant J 44(2): 334-341.
23. Hoffmann T, Kalinowski G, Schwab W (2006) RNAi-induced silencing of gene expression in strawberry fruit (Fragaria × ananassa) by agroinfiltration: a rapid assay for gene function analysis. Plant J 48(5): 818-826.
24. Jiang C, Lu F, Imsabai W, Meir S, Reid MS (2008) Silencing polygalacturonase expression inhibits tomato petiole abscission. J Exp Bot 59(4): 973-979.
25. Jiang C, Chen J, Reid M (2011) Virus-induced gene silencing in ornamental plants. In: RNAi and plant gene function analysis. Springer, pp 81-96.
26. Kumagai M, Donson J, Della-Cioppa G, Harvey D, Hanley K, Grill L (1995) Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci U S A 92(5): 1679-1683.
27. Li J, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive protein-based artificial microRNA screens for effective gene silencing in plants. Plant Cell 25(5): 1507-1522.
28. Liu YL, Schiff M, Dinesh-Kumar SP (2002a) Virus-induced gene silencing in tomato. Plant J 31(6): 777-786.
29. Liu YL, Schiff M, Marathe R, Dinesh-Kumar SP (2002b) Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J 30(4): 415-429.
30. Lu R, Martin-Hernandez AM, Peart JR, Malcuit I, Baulcombe DC (2003) Virus-induced gene silencing in plants. Methods 30(4): 296-303.
31. Mallona I, Lischewski S, Weiss J, Hause B, Egea-Cortines M (2010) Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida. BMC Plant Biol 10: 4.
32. Muruganantham M, Moskovitz Y, Haviv S et al (2009) Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera. J Virol Methods 155(2): 167-174.
33. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2(4): 279-289.
34. Qu F, Ye X, Hou G, Sato S, Clemente TE, Morris TJ (2005) RDR6 has a broad-spectrum but temperature-dependent antiviral defense role in Nicotiana benthamiana. J Virol 79(24): 15209-15217.
35. Ryu C, Anand A, Kang L, Mysore K (2004) Agrodrench: a novel and effective agroinoculation method for virus-induced gene silencing in roots and diverse solanaceous species. Plant J 40(2): 322-331.
36. Saedler R, Baldwin I (2004) Virus-induced gene silencing of jasmonate-induced direct defences, nicotine and trypsin proteinase-inhibitors in Nicotiana attenuata. J Exp Bot 55(395): 151-157.
37. Senthil-Kumar M, Hema R, Anand A, Kang L, Udayakumar M, Mysore KS (2007) A systematic study to determine the extent of gene silencing in Nicotiana benthamiana and other Solanaceae species when heterologous gene sequences are used for virus-induced gene silencing. New Phytol 176(4): 782-791.
38. Sharma B, Kramer EM (2013) Virus-induced gene silencing in the papid cycling columbine Aquilegia coerulea "Origami". In: Virus-induced gene silencing. Springer, New York, pp 71-81.
39. Sharma PC, Ito A, Shimizu T, Terauchi R, Kamoun S, Saitoh H (2003) Virus-induced silencing of WIPK and SIPK genes reduces resistance to a bacterial pathogen, but has no effect on the INF1-induced hypersensitive response (HR) in Nicotiana benthamiana. Mol Genet Genomics 269(5): 583-591.
40. Spitzer B, Zvi MMB, Ovadis M et al (2007) Reverse genetics of floral scent: application of tobacco rattle virus-based gene silencing in petunia. Plant Physiol 145(4): 1241-1250.
41. Spitzer-Rimon B, Marhevka E, Barkai O et al (2010) EOBII, a gene encoding a flower-specific regulator of phenylpropanoid volatiles' biosynthesis in petunia. Plant Cell 22(6): 1961-1976.
42. Szittya G, Silhavy D, Molnar A et al (2003) Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. EMBO J 22(3): 633-640.
43. Urso S, Zottini M, Ruberti C et al (2013) An Agrobacterium tumefaciens-mediated gene silencing system for functional analysis in grapevine. Plant Cell Tiss Org 114(1): 49-60.
44. van Kammen A (1997) Virus-induced gene silencing in infected and transgenic plants. Trends Plant Sci 2(11): 409-411.
45. Wang C, Cai X, Wang X, Zheng Z (2006) Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis. Funct Plant Biol 33(4): 347-355.
46. Watson JM, Fusaro AF, Wang M, Waterhouse PM (2005) RNA silencing platforms in plants. FEBS Lett 579(26): 5982-5987.
47. Wege S, Scholz A, Gleissberg S, Becker A (2007) Highly efficient virus-induced gene silencing (VIGS) in California poppy (Eschscholzia californica): an evaluation of VIGS as a strategy to obtain functional data from non-model plants. Ann Bot 100(3): 641-649.
48. Wu C, Jia L, Goggin F (2011) The reliability of virus-induced gene silencing experiments using tobacco rattle virus in tomato is influenced by the size of the vector control. Mol Plant Pathol 12(3): 299-305.
49. Xu X, Jiang C, Donnelly L, Reid MS (2007) Functional analysis of a RING domain ankyrin repeat protein that is highly expressed during flower senescence. J Exp Bot 58(13): 3623-3630.
50. Yan H, Fu D, Zhu B, Liu H, Shen X, Luo Y (2012) Sprout vacuum-infiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species. Plant Cell Rep 31(9): 1713-1722.