Transcriptional and posttranscriptional plant gene silencing in response to a pathogen

Science

Volumn 279, Issue 5359, 1998, Pages 2113-2115

  Al Kaff, Nadia S ^a   Covey, Simon N ^a   Kreike, Maria M ^a   Page, Anthony M ^a   Pinder, Rachel ^a   Dale, Philip J ^a  

^a JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AGRICULTURE; ARTICLE; BRASSICA; CAULIFLOWER MOSAIC VIRUS; GENE REPRESSION; NONHUMAN; PRIORITY JOURNAL; SILENT GENE; TRANSGENIC PLANT; VIRUS INFECTION;

EID: 0032571483     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5359.2113     Document Type: Article

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24. In situ hybridization to detect CaMV was performed as described (15) with the same leaf disks as those used for histochemical detection of GUS activity.
25. We thank N. Owen and C. Jones for producing transgenic lines, J. Jones for transformation constructs, and A. Lángara for advice on nuclear run-on assays. This work was supported by the U.K. Biotechnology and Biological Sciences Research Council and covered by license PHF 1491/982/34 of the Ministry of Agriculture, Fisheries and Food.