A recombinant estrogen receptor fragment-based homogeneous fluorescent assay for rapid detection of estrogens

Biosensors and Bioelectronics

Volumn 55, Issue , 2014, Pages 391-395

  Wang, Dan ^a   Xie, Jiangbi ^a   Zhu, Xiaocui ^a   Li, Jinqiu ^a   Zhao, Dongqin ^a   Zhao, Meiping ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

17 Estradiol; Coumestrol; Estrogen receptor; Fluorescent assay; Homogeneous fluorescent assay; Neuron culture medium

Indexed keywords

COUMESTROL; ESTROGEN RECEPTOR; FLUORESCENT ASSAYS; HIPPOCAMPAL NEURONS; NEURON CULTURE; PROKARYOTIC EXPRESSION; SENSITIVE DETECTION; SPECTROSCOPIC PROPERTY;

AMINO ACIDS; BINDING ENERGY; PROTEINS;

FLUORESCENCE;

AMINO ACID; COUMESTROL; ESTRADIOL; ESTROGEN; ESTROGEN RECEPTOR ALPHA; PHYTOESTROGEN; POLYHISTIDINE TAG; POLYPEPTIDE; RECOMBINANT PROTEIN; STREP TAG II; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; HIPPOCAMPAL NEURONAL CULTURE; HOMOGENEOUS FLUORESCENT ASSAY; HORMONE DETERMINATION; LIMIT OF DETECTION; NONHUMAN; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; RAT; RECEPTOR BINDING; SPECTROSCOPY; UPREGULATION; WILD TYPE;

17Β-ESTRADIOL; COUMESTROL; ESTROGEN RECEPTOR; FLUORESCENT ASSAY; HOMOGENEOUS FLUORESCENT ASSAY; NEURON CULTURE MEDIUM;

BIOSENSING TECHNIQUES; ESTROGEN RECEPTOR ALPHA; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, ESTROGEN; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 84892458440     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2013.12.050     Document Type: Article

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