Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses

Biochemical and Biophysical Research Communications

Volumn 443, Issue 2, 2014, Pages 568-573

  Ibey, Bennett L ^a   Ullery, Jody C ^b   Pakhomova, Olga N ^c   Roth, Caleb C ^b,d   Semenov, Iurii ^c   Beier, Hope T ^a   Tarango, Melissa ^b   Xiao, Shu ^c   Schoenbach, Karl H ^c   Pakhomov, Andrei G ^c  

^a Radio Frequency Bioeffects Branch Bioeffects Division 711th Human Performance Wing   (United States)

^b Dynamic Graphics Incorporated   (United States)

^c OLD DOMINION UNIVERSITY   (United States)

^d UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

Bipolar; Calcium; CHO; FM1 43; Monopolar; Nanosecond electric pulse

Indexed keywords

CALCIUM; DYE; FLUORESCEIN ISOTHIOCYANATE; LIPOCORTIN 5; PROPIDIUM IODIDE;

ANIMAL CELL; ARTICLE; BIPOLAR NANOSECOND PULSED ELECTRIC FIELD; CALCIUM CELL LEVEL; CELL DEATH; CELL KILLING; CELL MEMBRANE PERMEABILITY; CELL PERMEABILIZATION; CELL STRAIN U937; CELL SURVIVAL; CHO CELL; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ELECTROPORATION; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; LEUKEMIA CELL LINE; LONG TERM EXPOSURE; MEMBRANE STRUCTURE; MONOPOLAR NANOSECOND PULSED ELECTRIC FIELD; NONHUMAN; PRIORITY JOURNAL; PULSED ELECTRIC FIELD; SURVIVAL TIME;

CRICETULUS GRISEUS;

BIPOLAR; CALCIUM; CHO; FM1-43; MONOPOLAR; NANOSECOND ELECTRIC PULSE;

ANIMALS; APOPTOSIS; CELL MEMBRANE PERMEABILITY; CHO CELLS; CRICETINAE; CRICETULUS; DOSE-RESPONSE RELATIONSHIP, RADIATION; ELECTRIC STIMULATION; ELECTROMAGNETIC FIELDS; ELECTROPORATION; HUMANS; JURKAT CELLS; RADIATION DOSAGE;

EID: 84892437913     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.12.004     Document Type: Article

References (29)

1. J. Teissie et al. Electropermeabilization of cell membranes Adv. Drug Deliv. Rev. 35 1 1999 3 19
2. J.C. Weaver, and Y.A. Chizmadzhev Theory of electroporation: a review Bioelectrochem. Bioenerg. 41 1996 135 160
3. R. Davalos, L. Mir, and B. Rubinsky Tissue Ablation with Irreversible electroporation Ann. Biomed. Eng. 33 2 2005 223 231
4. B. Rubinsky Irreversible Electroporation Series in Biomedical Engineering 2010 Springer-Verlag Berlin Heidelberg
5. E. Tekle, R.D. Astumian, and P.B. Chock Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells Proc. Natl. Acad. Sci. 88 10 1991 4230 4234
6. T. Kotnik et al. Cell membrane electropermeabilization by symmetrical bipolar rectangular pulses: Part I. Increased efficiency of permeabilization Bioelectrochemistry 54 1 2001 83 90
7. C.B. Arena et al. High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction Biomed. Eng. Online 10 1 2011 102
8. I. Daskalov, N. Mudrov, and E. Peycheva Exploring new instrumentation parameters for electrochemotherapy. Attacking tumors with bursts of biphasic pulses instead of single pulses IEEE Eng. Med. Biol. Mag. 18 1 1999 62 66
9. C.B. Arena et al. Theoretical considerations of tissue electroporation with high-frequency bipolar pulses IEEE Trans. Biomed. Eng. 58 5 2011 1474 1482
10. S.J. Beebe et al. Transient features in nanosecond pulsed electric fields differentially modulate mitochondria and viability PLoS One 7 12 2012 e51349
11. A.G. Pakhomov et al. Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane Biochem. Biophys. Res. Commun. 385 2 2009 181 186
12. A.G. Pakhomov, and O.N. Pakhomova Nanopores: a distinct transmembrane passageway in electroporated cells A.G. Pakhomov, D. Miklavcic, M.S. Markov, Advanced Electroporation Techniques in Biology in Medicine 2010 CRC Press
13. K.S. Schoenbach et al. Bioelectric effects of nanosecond pulses IEEE Trans. Dielectr. Electr. Insul. 14 5 2007 1088 1109
14. K.H. Schoenbach et al. Bioelectrics - new applications for pulsed power technology IEEE Trans. Plasma Sci. 30 1 2002 293 300
15. P.T. Vernier, Y. Sun, and M.A. Gundersen Nanoelectropulse-driven membrane perturbation and small molecule permeabilization BMC Cell Biol. 7 2006 37
16. D.M. French et al. Conductive versus capacitive coupling for cell electroporation with nanosecond pulses J. Appl. Phys. 106 7 2009 074701 074704
17. K.C. Smith, and J.C. Weaver Active mechanisms are needed to describe cell responses to submicrosecond, megavolt-per-meter pulses: cell models for ultrashort pulses Biophys. J. 95 4 2008 1547 1563
18. J.C. Weaver et al. A brief overview of electroporation pulse strength duration space: a region where additional intracellular effects are expected Bioelectrochemistry 87 2012 236 243
19. K.C. Smith, J.C. Neu, and W. Krassowska Model of creation and evolution of stable electropores for DNA delivery Biophys. J. 86 5 2004 2813 2826
20. T. Kotnik et al. Role of pulse shape in cell membrane electropermeabilization Biochim. Biophys. Acta (BBA) - Biomembr. 1614 2 2003 193 200
21. T. Kotnik, and D. Miklavcic Theoretical evaluation of voltage inducement on internal membranes of biological cells exposed to electric fields Biophys. J. 90 2 2006 480 491
22. K.H. Schoenbach, S.J. Beebe, and E.S. Buescher Intracellular effect of ultrashort electrical pulses Bioelectromagnetics 22 6 2001 440 448
23. S.J. Beebe et al. Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cells FASEB J. 17 11 2003 1493 1495
24. S. Beebe, N. Sain, and W. Ren Induction of cell death mechanisms and apoptosis by nanosecond pulsed electric fields (nsPEFs) Cells 2 1 2013 136 162
25. T.B. Napotnik et al. Nanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells Bioelectromagnetics 33 3 2012 257 264
26. 2+ mobilization by nanosecond pulsed electric field Biochim. Biophys. Acta (BBA) - Biomembr. 1828 2013 981 989
27. 2+ by ultrashort electric stimuli: The impact of pulse duration Cell Calcium 54 2013 145 150
28. B.L. Ibey et al. Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell lines PLoS One 6 1 2011 e15642
29. O.N. Pakhomova et al. Two modes of cell death caused by exposure to nanosecond pulsed electric field PLoS One 8 7 2013 e70278