Optimization of ERK activity biosensors for both ratiometric and lifetime FRET measurements

Sensors (Switzerland)

Volumn 14, Issue 1, 2014, Pages 1140-1154

  Vandame, Pauline ^a   Spriet, Corentin ^a   Riquet, Franck ^a   Trinel, Dave ^a   Cailliau Maggio, Katia ^a   Bodart, Jean François ^a  

^a UNIV LILLE   (France)

Author keywords

ERK; FRET; Genetically encoded biosensor

Indexed keywords

ENZYMES; EXPERIMENTS; FLUORESCENCE; FLUOROPHORES; OPTIMIZATION; PHOSPHORYLATION; SIGNAL TRANSDUCTION; TOOLS;

CONFORMATIONAL CHANGE; ENZYMATIC ACTIVITIES; ERK; FLUORESCENCE LIFETIMES; FLUORESCENCE PROPERTIES; FRET; SIGNAL TRANSDUCTION PATHWAYS; SPECIFIC SUBSTRATES;

BIOSENSORS;

EID: 84892390603     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s140101140     Document Type: Article

References (22)

1. Bodart, J. F. Extracellular-regulated kinase-mitogen-activated protein kinase cascade: Unsolved issues. J. Cell Biochem. 2010, 109, 850-857.
2. Wang, R.; He, G.; Nelman-Gonzalez, M.; Ashorn, C. L.; Gallick, G. E.; Stukenberg, P. T.; Kirschner, M. W.; Kuang, J. Regulation of Cdc25C by ERK-MAP kinases during the G2/M transition. Cell 2007, 128, 1119-1132.
3. Yoon, S.; Seger, R. The extracellular signal-regulated kinase: Multiple substrates regulate diverse cellular functions. Growth Factors 2006, 24, 21-44.
4. Santos, S. D.; Verveer, P. J.; Bastiaens, P. I. Growth factor-induced MAPK network topology shapes Erk response determining PC-12 cell fate. Nat. Cell Biol. 2007, 9, 324-330.
5. Ferrell, J. E., Jr.; Machleder, E. M. The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes. Science 1998, 280, 895-898.
6. Beaujois, R.; Riquet, F.; Cailliau, K.; Browayes, E.; Russo, C.; Blossey, R.; Vicogne, D.; Marin, M.; Lescuyer-Rousseau, A.; Vilain, J. P.; et al. Ultrasensitive MAPK/Erk activation in absence of protein synthesis in Xenopus oocytes. MAP Kinase 2013, 2, 1-9.
7. Russo, C.; Giuraniuc, C.; Blossey, R.; Bodart, J. F. On the equilibria of the MAPK cascade: Cooperativity, modularity and bistability. Phys. A Stat. Mech. Appl 2009, 388, 5070-5080.
8. Pullikuth, A. K.; Catling, A. D. Scaffold mediated regulation of MAPK signaling and cytoskeletal dynamics: A perspective. Cell Signal 2007, 19, 1621-1632.
9. Sipieter, F.; Vandame, P.; Spriet, C.; Leray, A.; Vincent, P.; Trinel, D.; Bodart, J. F.; Riquet, F. B.; Heliot, L. From FRET imaging to practical methodology for kinase activity sensing in living cells. Prog. Mol. Biol. Transl. Sci. 2013, 113, 145-216.
10. Kardash, E.; Bandemer, J.; Raz, E. Imaging protein activity in live embryos using fluorescence resonance energy transfer biosensors. Nat. Protoc. 2011, 6, 1835-1846.
11. Fujioka, A.; Terai, K.; Itoh, R. E.; Aoki, K.; Nakamura, T.; Kuroda, S.; Nishida, E.; Matsuda, M. Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. J. Biol. Chem. 2006, 281, 8917-8926.
12. Tsuboi, Y.; Ichida, T.; Sugitani, S.; Genda, T.; Inayoshi, J.; Takamura, M.; Matsuda, Y.; Nomoto, M.; Aoyagi, Y. Overexpression of extracellular signal-regulated protein kinase and its correlation with proliferation in human hepatocellular carcinoma. Liver Int. 2004, 24, 432-436.
13. Sato, M.; Kawai, Y.; Umezawa, Y. Genetically encoded fluorescent indicators to visualize protein phosphorylation by extracellular signal-regulated kinase in single living cells. Anal. Chem. 2007, 79, 2570-2575.
14. Harvey, C. D.; Ehrhardt, A. G.; Cellurale, C.; Zhong, H.; Yasuda, R.; Davis, R. J.; Svoboda, K. A genetically encoded fluorescent sensor of ERK activity. Proc. Nat. Acad. Sci. USA 2008, 105, 19264-19269.
15. Eymin, B.; Claverie, P.; Salon, C.; Brambilla, C.; Brambilla, E.; Gazzeri, S. p14ARF triggers G2 arrest through ERK-mediated Cdc25C phosphorylation, ubiquitination and proteasomal degradation. Cell Cycle 2006, 5, 759-765.
16. Komatsu, N.; Aoki, K.; Yamada, M.; Yukinaga, H.; Fujita, Y.; Kamioka, Y.; Matsuda, M. Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol. Biol. Cell 2011, 22, 4647-4656.
17. Klarenbeek, J. B.; Goedhart, J.; Hink, M. A.; Gadella, T. W.; Jalink, K. A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range. PLoS One 2011, 6, e19170.
18. Depry, C.; Allen, M. D.; Zhang, J. Visualization of PKA activity in plasma membrane microdomains. Mol. Biosyst. 2011, 7, 52-58.
19. Allen, M. D.; Zhang, J. Subcellular dynamics of protein kinase a activity visualized by FRET-based reporters. Biochem. Biophys. Res. Commun. 2006, 348, 716-721.
20. Goedhart, J.; van Weeren, L.; Hink, M. A.; Vischer, N. O.; Jalink, K.; Gadella, T. W., Jr. Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Nat. Methods 2010, 7, 137-139.
21. Kremers, G. J.; Goedhart, J.; van Munster, E. B.; Gadella, T. W., Jr. Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Forster radius. Biochemistry 2006, 45, 6570-6580.
22. Fritz, R. D.; Letzelter, M.; Reimann, A.; Martin, K.; Fusco, L.; Ritsma, L.; Ponsioen, B.; Fluri, E.; Schulte-Merker, S.; van Rheenen, J.; et al. A versatile toolkit to produce sensitive FRET biosensors to visualize signaling in time and space. Sci. Signal 2013, 6, 12.