Assessment of five soil DNA extraction methods and a rapid laboratory-developed method for quality soil DNA extraction for 16S rDNA-based amplification and library construction

Journal of Microbiological Methods

Volumn 97, Issue 1, 2014, Pages 68-73

  Sagar, Kalpana ^a   Singh, Salam Pradeep ^a   Goutam, Kapil Kumar ^a   Konwar, Bolin Kumar ^a  

^a TEZPUR UNIVERSITY   (India)

Author keywords

Chimeric; Humic substances; Metagenomic DNA

Indexed keywords

BACTERIAL DNA; DNA 16S; DNA FRAGMENT; SEPHADEX;

ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; DNA LIBRARY; DNA PURIFICATION; ESCHERICHIA COLI; GENE AMPLIFICATION; HUMIC SUBSTANCE; INTERMETHOD COMPARISON; METAGENOMICS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; SOIL;

CHIMERIC; HUMIC SUBSTANCES; METAGENOMIC DNA;

DNA, BACTERIAL; GENE LIBRARY; HUMIC SUBSTANCES; MICROBIOLOGICAL TECHNIQUES; RNA, RIBOSOMAL, 16S; SOIL; SOIL MICROBIOLOGY;

EID: 84892368136     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.11.008     Document Type: Article

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