Comparison of the stratum corneum thickness measured in vivo with confocal Raman spectroscopy and confocal reflectance microscopy

Skin Research and Technology

Volumn 20, Issue 1, 2014, Pages 50-57

  Bohling A ^a   Bielfeldt, S ^a   Himmelmann, A ^a   Keskin, M ^a   Wilhelm, K P ^a  

^a proDERM Institute for Applied Dermatological Research GmbH   (Germany)

Author keywords

Confocal laser scanning microscopy; Confocal Raman spectroscopy; SC thickness; Stratum corneum; Water refraction index matching fluid

Indexed keywords

CONFOCAL LASER SCANNING MICROSCOPY; CONFOCAL RAMAN SPECTROSCOPY; CONFOCAL REFLECTANCE MICROSCOPIES; IN-VIVO MEASUREMENT; INDEX-MATCHING; MOLECULAR COMPOSITIONS; STRATUM CORNEUM; STRUCTURE ANALYSIS;

BIOCHEMISTRY; MOLECULAR ORIENTATION; THICKNESS MEASUREMENT;

CONFOCAL MICROSCOPY;

ACCURACY; ADULT; ARTICLE; CHEEK; CLINICAL ARTICLE; COMPARATIVE STUDY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FACE; FEMALE; FOREARM; HAND PALM; HUMAN; LOWER LEG; MALE; MEASUREMENT; MIDDLE AGED; NORMAL HUMAN; RAMAN SPECTROMETRY; STRATUM CORNEUM; STRUCTURE ANALYSIS; THICKNESS;

CONFOCAL LASER SCANNING MICROSCOPY; CONFOCAL RAMAN SPECTROSCOPY; SC THICKNESS; STRATUM CORNEUM; WATER REFRACTION INDEX-MATCHING FLUID;

ADULT; DERMOSCOPY; EPIDERMIS; HUMANS; MICROSCOPY, CONFOCAL; MIDDLE AGED; ORGAN SIZE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTRUM ANALYSIS, RAMAN;

EID: 84892367125     PISSN: 0909752X     EISSN: 16000846     Source Type: Journal    
DOI: 10.1111/srt.12082     Document Type: Article

References (26)

1. Elias PM. Stratum corneum defensive functions: an integrated view. J Invest Dermatol 2005; 125: 183-200.
2. Rawlings AV, Harding CR. Moisturization and skin barrier function. Dermatol Ther 2004; 17(Suppl 1): 43-48.
3. Holbrook KA, Odland GF. Regional differences in the thickness (cell layers) of the human stratum corneum: an ultrastructural analysis. J Invest Dermatol 1974; 62: 415-422.
4. Ya-Xian Z, Suetake T, Tagami H. Number of cell layers of the stratum corneum in normal skin - relationship to the anatomical location on the body, age, sex and physical parameters. Arch Dermatol Res 1999; 291: 555-559.
5. Guérif-Ferreira Y, Oberto G, Berghi A, Cucumel K, Dal Farra C, Domloge N. In vivo study of age-related skin change through in vivo confocal microscopy. Poster Presentation: 70th Annual Meeting of the Society for Investigative Dermatology. Atlanta, Georgia, 2010: P291.
6. Imbert I, Clay AF, Mantelin J, Domloge N. Pisum sativum extract for safe- and self-tanning. Cosmet Toiletries 2009; 124: 34-41.
7. Imbert I, Guerif-Ferreira Y, Oberto G, Berghi A, Mouser P, Cucumel K, Domloge N. In vivo study of age-related skin changes using in vivo confocal microscopy. IFSCC 2012; 1: 15-21.
8. Bielfeldt S, Böhling A, Wilhelm K-P. Bioengineering methods to assess aging parameters in the depth of the skin. SOFW J 2011; 137: 2-9.
9. Zhang SL, Caspers PJ, Puppels GJ. In vivo confocal Raman microspectroscopy of the skin: effect of skin care products on molecular concentration depth-profiles. Microsc Microanal 2005; 11: 790-791.
10. Sieg A, Crowther J, Blenkiron P, Marcott C, Matts PJ. Confocal Raman microspectroscopy: measuring the effects of topical moisturisers on stratum corneum water gradient, in vivo. Conference 6093 - Biomedical Vibrational Spectroscopy III: Advances in Research and Industry. San Jose, California, USA, 2006 (January 21-22).
11. Kaya G, Saurat JH. Dermatoporosis: a chronic cutaneous insufficiency/fragility syndrome. Clinicopathological features, mechanisms, prevention and potential treatments. Dermatology 2007; 215: 284-294.
12. Chrit L, Hadjur C, Morel S, Sockalingum G, Lebourdon G, Leroy F, Manfait M. In vivo chemical investigation of human skin using a confocal Raman fiber optic microprobe. J Biomed Opt 2005; 10: 1-11.
13. Kalia YN, Alberti I, Naik A, Guy RH. Assessment of topical bioavailability in vivo: the importance of stratum corneum thickness. Skin Pharmacol Appl Skin Physiol 2001; 14(Suppl 1): 82-86.
14. Herkenne C, Naik A, Kalia YN, Hadgraft J, Guy RH. Dermatopharmacokinetic prediction of topical drug bioavailability in vivo. J Invest Dermatol 2007; 127: 887-894.
15. Caspers PJ, Lucassen GW, Carter EA, Bruining HA, Puppels GJ. In vivo confocal Raman microspectroscopy of the skin: noninvasive determination of molecular concentration profiles. J Invest Dermatol 2001; 116: 434-442.
16. Bielfeldt S, Schoder V, Ely U, Van der Pol A, de Sterke J, Wilhelm KP. Assessment of human stratum corneum thickness and its barrier properties by in vivo confocal Raman spectroscopy. IFSCC Mag 2009; 12: 9-15.
17. Zha WF, Song WM, Ai JJ, Xu AE. Mobile connected dermatoscope and confocal laser scanning microscope: a useful combination applied in facial simple sensitive skin. Int J Cosmet Sci 2012; 34: 318-321.
18. Pellacani G, Longo C. In vivo noninvasive skin evaluation. Expr Cosmét 2011; 11: 110-116.
19. Rudnicka L, Olszewska M, Rakowksa A. In vivo reflectance confocal microscopy: usefulness for diagnosing hair diseases. J Dermatol Case Rep 2008; 2: 55-59.
20. Altintas MA, Altintas AA, Guggenheim M, Niederbichler AD, Knobloch K, Vogt PM. In vivo evaluation of histomorphological alterations in first-degree burn injuries by means of confocal-laser-scanning microscopy-more than "virtual histology?". J Burn Care Res 2009; 30: 315-320.
21. Caspers PJ, Lucassen GW, Bruining HA, Puppels GJ. Automated depth-scanning confocal Raman microspectrometer for rapid in vivo determination of water concentration profiles in human skin. J Raman Spectrosc 2000; 31: 813-818.
22. Van der Pol A, de Sterke J, Caspers PJ. Modeling and interpretation of water concentration gradients in stratum corneum as measured by confocal Raman microspectroscopy. Stratum Corneum V, session: New investigative methods ISBS Symposium. Cardiff, UK, 2007.
23. Böhling A, Bielfeldt S, Wilhelm KP. Advances in measuring the water content of dry skin by in vivo confocal raman spectroscopy. ISBS Meeting: Abstract for podium or poster presentation in session "emerging technologies". Tampa, FL, USA, 2011.
24. Huzaira M, Rius F, Rajadhyaksha M, Anderson RR, Gonzalez S. Topographic variations in normal skin, as viewed by in vivo reflectance confocal microscopy. J Invest Dermatol 2001; 116: 846-852.
25. Robertson K, Rees JL. Variation in epidermal morphology in human skin at different body sites as measured by reflectance confocal microscopy. Acta Derm Venereol 2010; 90: 368-373.
26. Egawa M, Hirao T, Takahashi M. In vivo estimation of stratum corneum thickness from water concentration profiles obtained with Raman spectroscopy. Acta Derm Venereol 2007; 87: 4-8.