Cloning, expression, purification and characterization of his-tagged human glucose-6-phosphate dehydrogenase: A simplified method for protein yield

Protein Journal

Volumn 32, Issue 7, 2013, Pages 585-592

  Gómez Manzo, Saúl ^a   Terrón Hernandez, Jessica ^a   De La Mora De La Mora, Ignacio ^a   García Torres, Itzhel ^a   Lopez Velázquez, Gabriel ^a   Reyes Vivas, Horacio ^a   Oria Hernández, Jesus ^a  

^a SECRETARÍA DE SALUD   (Mexico)

Author keywords

G6Pd deficiency; IMAC; Protein function; Protein stability; Protein structure; Recombinant protein

Indexed keywords

GLUCOSE 6 PHOSPHATE DEHYDROGENASE;

ARTICLE; BACTERIUM CULTURE; ESCHERICHIA COLI; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PENTOSE PHOSPHATE CYCLE; POINT MUTATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; RESTRICTION SITE; RNA ISOLATION;

CHROMATOGRAPHY, AFFINITY; GLUCOSEPHOSPHATE DEHYDROGENASE; HISTIDINE; HUMANS; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS;

EID: 84892177140     PISSN: 15723887     EISSN: 18758355     Source Type: Journal    
DOI: 10.1007/s10930-013-9518-x     Document Type: Article

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