What is the role of the second "structural" NADP +-binding site in human glucose 6-phosphate dehydrogenase?

Protein Science

Volumn 17, Issue 8, 2008, Pages 1403-1411

  Wang, Xiao Tao ^a,b   Ting, Fai Chan ^a   Lam, Veronica M S ^a   Engel, Paul C ^b  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

"structural" NADP+; Apoenzyme; Cofactor removal; Dissociation constant; Enzyme stability; G6PD deficiency; Glucose 6 phosphate dehydrogenase

Indexed keywords

APOENZYME; DIMER; GLUCOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; HOLOENZYME; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BINDING SITE; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; EQUILIBRIUM CONSTANT; GLUCOSE 6 PHOSPHATE DEHYDROGENASE DEFICIENCY; HUMAN; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

BINDING SITES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GLUCOSEPHOSPHATE DEHYDROGENASE; GLUCOSEPHOSPHATE DEHYDROGENASE DEFICIENCY; HUMANS; KINETICS; MODELS, MOLECULAR; MOLECULAR WEIGHT; MUTANT PROTEINS; NADP; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TRICHLOROACETIC ACID;

EID: 48249100845     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.035352.108     Document Type: Article

References (32)

1. + molecule and provides insights into enzyme deficiency. Structure 8: 293-303.
2. Baronciani, L., Tricta, F., and Beutler, E. 1993. G6PD "Campinas": A deficient enzyme with a mutation at the far 3′ end of the gene. Hum. Mutat. 2: 77-78.
3. Betke, K., Beutler, E., Brewer, G.J., Kirkman, H.N., Luzzatto, L., Motulsky, A.G., Ramot, B., and Siniscolo, M. 1967. Standardisation of procedures for the study of glucose-6-phosphate dehydrogenase. Report of a WHO scientific group. WHO Tech. Rep. Ser. 366: 1-53.
4. Beutler, E. and Collins, Z. 1965. Hybridization of glucose-6-phosphate dehydrogenase from rat and human erythrocytes. Science 150: 1306-1307.
5. Bonsignore, A., Lorenzoni, I., Cancedda, R., and De Flora, A. 1970. Distinctive patterns of NADP binding to dimeric and tetrameric glucose 6-phosphate dehydrogenase from human red cells. Biochem. Biophys. Res. Commun. 39: 142-148.
6. Bonsignore, A., Cancedda, R., Nicolini, A., Damiani, G., and De Flora, A. 1971a. Metabolism of human erythrocyte glucose-6-phosphate dehydrogenase. VI. Interconversion of multiple molecular forms. Arch. Biochem. Biophys. 147: 493-501.
7. Bonsignore, A., Cancedda, R., Lorenzoni, I., Cosulich, M.E., and De Flora, A. 1971b. Human erythrocyte glucose 6-phosphate dehydrogenase. Physical properties. Biochem. Biophys. Res. Commun. 43: 94-101.
8. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
9. + and NADPH. Eur. J. Biochem. 34: 199-204.
10. Chung, A.E. and Langdon, R.G. 1963. Human erythrocyte glucose 6-phosphate dehydrogenase. II. Enzyme-coenzyme interrelationship. J. Biol. Chem. 238: 2317-2324.
11. Cohen, P. and Rosemeyer, M.A. 1969. Subunit interactions of human glucose 6-phosphate dehydrogenase from human erythrocytes. Eur. J. Biochem. 8: 8-15.
12. De Flora, A., Morelli, A., and Giuliano, F. 1974. Human glucose 6-phosphate dehydrogenase: Content of bound coenzyme. Biochem. Biophys. Res. Commun. 59: 406-413.
13. Gomez-Gallego, F., Garrido-Pertierra, A., Mason, P.J., and Bautista, J.M. 1996. Unproductive folding of the human G6PD-deficient variant A-. FASEB J. 10: 153-158.
14. + of human glucose-6-phosphate dehydrogenase by sequence analysis of mutants. Proc. Natl. Acad. Sci. 86: 10015-10017.
15. Kirkman, H.N. 1962. Glucose 6-phosphate dehydrogenase from human erythrocytes. I. Further purification and characterization. J. Biol. Chem. 237: 2364-2370.
16. Kirkman, H.N. and Gaetani, G.F. 1986. Regulation of glucose-6-phosphate dehydrogenase in human erythrocytes. J. Biol. Chem. 261: 4033-4038.
17. Kirkman, H.N. and Hendrickson, E.M. 1962. Glucose 6-phosphate dehydrogenase from human erythrocytes. II. Subactive states of the enzyme from normal persons. J. Biol. Chem. 237: 2371-2376.
18. + binding to human glucose-6-phosphate dehydrogenase. Acta Crystallogr. D Biol. Crystallogr. 61: 495-504.
19. Landt, O., Grunert, H.P., and Hahn, U.A. 1990. A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene 96: 125-128.
20. Luzzatto, L. and Allan, N.C. 1965. Different properties of glucose 6-phosphate dehydrogenase from human erythrocytes with normal and abnormal enzyme levels. Biochem. Biophys. Res. Commun. 21: 547-554.
21. Mason, P.J., Bautista, J.M., and Gilsanz, F. 2007. G6PD deficiency: The genotype-phenotype association. Blood Rev. 21: 267-283.
22. Rowland, P., Basak, A.K., Gover, S., Levy, H.R., and Adams, M.J. 1994. The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 Å resolution. Structure 2: 1073-1087.
23. Sarkar, G. and Sommer, S.S. 1990. The "megaprimer" method of site-directed mutagenesis. Biotechniques 8: 404-407.
24. Scopes, D.A., Bautista, J.M., Naylor, C.E., Adams, M.J., and Mason, P.J. 1998. Amino acid substitutions at the dimer interface of human glucose-6-phosphate dehydrogenase that increase thermostability and reduce the stabilising effect of NADP. Eur. J. Biochem. 251: 382-388.
25. Vulliamy, T.J. and Luzzatto, L. 2003. Glucose-6-phosphate dehydrogenase deficiency and related disorders. In Blood: Principles and practice of hematology (eds. R.I. Handin et al.), pp. 1921-1950. Lippincott Williams and Wilkins, Philadelphia, PA.
26. Vulliamy, T., Luzzatto, L., Hirono, A., and Beutler, E. 1997. Hematologically important mutations: glucose-6-phosphate dehydrogenase. Blood Cells Mol. Dis. 23: 302-313.
27. Wang, X.T., Au, S.W.N., Lam, V.M.S., and Engel, P.C. 2002. Recombinant human glucose-6-phosphate dehydrogenase. Evidence for a rapid-equilibrium random-order mechanism. Eur. J. Biochem. 269: 3417-3424.
28. Wang, X.T., Lam, V.M.S., and Engel, P.C. 2005. Marked decrease in specific activity contributes to disease phenotype in two human glucose 6-phosphate dehydrogenase mutants, G6PD(Union) and G6PD(Andalus). Hum. Mutat. 26: 284.
29. Wang, X.T., Lam, V.M.S., and Engel, P.C. 2006. Functional properties of two mutants of human glucose 6-phosphate dehydrogenase, R393G and R393H, corresponding to the clinical variants G6PD Wisconsin and Nashville. Biochim. Biophys. Acta 1762: 767-774.
30. Wrigley, N.G., Heather, J.V., Bonsignore, A., and De Flora, A. 1972. Human erythrocyte glucose 6-phosphate dehydrogenase: Electron microscope studies on structure and interconversion of tetramers, dimers and monomers. J. Mol. Biol. 68: 483-499.
31. Yoshida, A. 1973. Hemolytic anemia and G6PD deficiency. Science 179: 532-537.
32. Yoshida, A., Steinmann, L., and Harbert, P. 1967. In vitro hybridization of normal and variant human glucose-6-phosphate dehydrogenase. Nature 216: 275-276.