Identification of secreted bacterial proteins by noncanonical amino acid tagging

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 1, 2014, Pages 433-438

  Mahdavi, Alborz ^a,b   Szychowski, Janek ^a   Ngo, John T ^a   Sweredoski, Michael J ^c   Graham, Robert L J ^c   Hess, Sonja ^c   Schneewind, Olaf ^d   Mazmanian, Sarkis K ^b   Tirrell, David A ^a  

^a California Institute of Technology   (United States)

^b California Institute of Technology   (United States)

^c CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^d UNIVERSITY OF CHICAGO   (United States)

Author keywords

BONCAT; Click chemistry; Proteomics; Yop

Indexed keywords

ALKYNE; AMINO ACID; AZIDE; AZIDONORLEUCINE; BACTERIAL PROTEIN; METHIONINE; METHIONINE TRANSFER RNA LIGASE; OUTER MEMBRANE PROTEIN A; PROTEIN TAG; PYROXYLIN; UNCLASSIFIED DRUG; VIRULENCE FACTOR; YERSINIA OUTER PROTEIN;

ARTICLE; BACTERIAL OUTER MEMBRANE; BACTERIAL VIRULENCE; CELL LABELING; CLICK CHEMISTRY; CONFOCAL MICROSCOPY; CYCLOADDITION; ENZYME ACTIVATION; FEMALE; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; HELA CELL; HOST CELL; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN SECRETION; TYPE III SECRETION SYSTEM; WESTERN BLOTTING; YERSINIA ENTEROCOLITICA; YERSINIA INFECTION;

BONCAT; CLICK CHEMISTRY; PROTEOMICS; YOP;

AMINO ACIDS; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; HELA CELLS; HUMANS; MASS SPECTROMETRY; METHIONINE-TRNA LIGASE; MICROBIOLOGICAL TECHNIQUES; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; MUTATION; NORLEUCINE; PROTEOME; PROTEOMICS; TIME FACTORS; VIRULENCE FACTORS; YERSINIA ENTEROCOLITICA;

EID: 84891948652     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1301740111     Document Type: Article

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