Silencing of DLGAP5 by siRNA significantly inhibits the proliferation and invasion of hepatocellular carcinoma cells

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Liao, Weijia ^a   Liu, Weilong ^b   Yuan, Qing ^c   Liu, Xing ^c   Ou, Ying ^c   He, Songqing ^a   Yuan, Shengguang ^a   Qin, Liling ^a   Chen, Qian ^a   Nong, Kate ^a   Mei, Minghui ^a   Huang, Jian ^a,b,c  

^a GUILIN MEDICAL UNIVERSITY   (China)

^b SHENZHEN THIRD PEOPLE S HOSPITAL   (China)

^c National Engineering Center for Biochip at Shanghai   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA FETOPROTEIN; SMALL INTERFERING RNA;

ADULT; ARTICLE; CANCER INHIBITION; CARCINOMA CELL; CELL INVASION; CELL MIGRATION; CELL PROLIFERATION; COLONY FORMATION; CONTROLLED STUDY; DLGAP5 GENE; FEMALE; GENE CONTROL; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENE SILENCING; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; LIVER CARCINOGENESIS; LIVER CELL CARCINOMA; MAJOR CLINICAL STUDY; MALE; MARKER GENE; MIDDLE AGED; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN METHYLATION; RNA INTERFERENCE; UPREGULATION;

CARCINOMA, HEPATOCELLULAR; CELL CYCLE; CELL LINE, TUMOR; CELL MOVEMENT; CELL PROLIFERATION; CELL TRANSFORMATION, NEOPLASTIC; DNA METHYLATION; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; LIVER; LIVER NEOPLASMS; MALE; MIDDLE AGED; NEOPLASM PROTEINS; PROMOTER REGIONS, GENETIC; RNA, SMALL INTERFERING;

EID: 84891900268     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0080789     Document Type: Article

References (25)

1. Tang ZY, Ye SL, Liu YK, Qin LX, Sun HC et al. (2004) A decade's studies on metastasis of hepatocellular carcinoma. J Cancer Res Clin Oncol 130: 187-196. doi:10.1007/s00432-003-0511-1. PubMed: 14685850.
2. Minagawa M, Makuuchi M, Takayama T, Kokudo N (2003) Selection criteria for repeat hepatectomy in patients with recurrent hepatocellular carcinoma. Ann Surg 238: 703-710. doi:10.1097/01.sla. 0000094549.11754.e6. PubMed: 14578733.
3. Poon RT, Fan ST, Lo CM, Liu CL, Wong J (1999) Intrahepatic recurrence after curative resection of hepatocellular carcinoma: long-term results of treatment and prognostic factors. Ann Surg 229: 216-222. doi:10.1097/00000658- 199902000-00009. PubMed: 10024103.
4. Tralhão JG, Dagher I, Lino T, Roudié J, Franco D (2007) Treatment of tumour recurrence after resection of hepatocellular carcinoma. Analysis of 97 consecutive patients. Eur J Surg Oncol 33: 746-751. doi:10.1016/j.ejso.2006.11.015. PubMed: 17188454.
5. Kobayashi A, Kawasaki S, Miyagawa S, Miwa S, Noike T et al. (2006) Results of 404 hepatic resections including 80 repeat hepatectomies for hepatocellular carcinoma. Hepatogastroenterology 53: 736-741. PubMed: 17086879.
6. Xu XR, Huang J, Xu ZG, Qian BZ, Zhu ZD et al. (2001) Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver. Proc Natl Acad Sci U S A 98: 15089-15094. doi:10.1073/pnas.241522398. PubMed: 11752456.
7. Huang J, Sheng HH, Shen T, Hu YJ, Xiao HS et al. (2006) Correlation between genomic DNA copy number alterations and transcriptional expression in hepatitis B virus-associated hepatocellular carcinoma. FEBS Lett 580: 3571-3581. doi:10.1016/j.febslet.2006.05.032. PubMed: 16750200.
8. Tsou AP, Yang CW, Huang CY, Yu RC, Lee YC et al. (2003) Identification of a cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. Oncogene 22: 298-307. doi:10.1038/sj.onc.1206129. PubMed: 12527899.
9. Verslype C, Rosmorduc O, Rougier P (2012) Hepatocellular carcinoma: ESMO-ESDO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol Suppl 7: vii41-vii48. PubMed: 22997453.
10. Tian J, Tang ZY, Ye SL, Liu YK, Lin ZY et al. (1999) New human hepatocellular carcinoma (HCC) cell line with highly metastatic potential (MHCC97) and its expressions of the factors associated with metastasis. Br J Cancer 81: 814-821. doi:10.1038/sj.bjc.6690769. PubMed: 10555751.
11. Li Y, Tang ZY, Ye SL, Liu YK, Chen J et al. (2002) Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection. National Medical Journal of China 82: 601-605. PubMed: 12133480.
12. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) met hod. Methods 25: 402-408. doi:10.1006/meth.2001.1262. PubMed: 11846609.
13. Du R, Lu C, Jiang Z, Li S, Ma R et al. (2012) Efficient typing of copy number variations in a segmental duplication-mediated rearrangement hotspot using multiplex competitive amplification. J Hum Genet 57: 545-551. doi:10.1038/jhg.2012.66. PubMed: 22673690.
14. Shi GM, Ke AW, Zhou J, Wang XY, Xu Y et al. (2010) CD151 modulates expression of matrix metalloproteinase 9 and promotes neoangiogenesis and progression of hepatocellular carcinoma. Hepatology 52: 183-196. doi:10.1016/S0168-8278(10)60453-9. PubMed: 20578262.
15. Yang XR, Xu Y, Yu B, Zhou J, Qiu SJ et al. (2010) High expression levels of putative hepatic stem/progenitor cell biomarkers related to tumour angiogenesis and poor prognosis of hepatocellular carcinoma. Gut 59: 953-962. doi:10.1136/gut.2008.176271. PubMed: 20442200.
16. Ryu HS, Park SH, Lee KB, Shin E, Jang JJ (2011) Expression of the Transmembrane Glycoprotein CD44s Is Potentially an Independent Predictor of Recurrence in Hepatocellular Carcinoma. Gut Liver 5: 204-209. doi:10.5009/gnl.2011.5.2.204. PubMed: 21814602.
17. Sun BS, Dong QZ, Ye QH, Sun HJ, Jia HL et al. (2008) Lentiviralmediated miRNA against osteopontin suppresses tumor growth and metastasis of human hepatocellular carcinoma. Hepatology 48: 1834-1842. doi:10.1002/hep.22531. PubMed: 18972404.
18. Li JC, Yang XR, Sun HX, Xu Y, Zhou J et al. (2010) Up-regulation of Krüppel-like factor 8 promotes tumor invasion and indicates poor prognosis for hepatocellular carcinoma. Gastroenterology 139: 2146-2157. doi:10.1053/j.gastro.2010.08.004. PubMed: 20728449.
19. Wu K, Ding J, Chen C, Sun W, Ning BF et al. (2012) Hepatic transforming growth factor beta gives rise to tumor-initiating cells and promotes liver cancer development. Hepatology 56: 2255-2267. doi: 10.1002/hep.26007. PubMed: 22898879.
20. Xiao Q, Qu K, Wang C, Kong Y, Liu C et al. (2013) HDGF-related protein-3 is required for anchorage-independent survival and chemoresistance in hepatocellular carcinomas. Gut 62: 440-451. doi: 10.1136/gutjnl-2011-300781. PubMed: 22490522.
21. Kuo TC, Chang PY, Huang SF, Chou CK, Chao CC (2012) Knockdown of HURP inhibits the proliferation of hepacellular carcinoma cells via downregulation of gankyrin and accumulation of p53. Biochem Pharmacol 83: 758-768. doi:10.1016/j.bcp.2011.12.034. PubMed: 22230478.
22. Fragoso MC, Almeida MQ, Mazzuco TL, Mariani BM, Brito LP et al. (2012) Combined expression of BUB1B, DLGAP5, and PINK1 as predictors of poor outcome in adrenocortical tumors: validation in a Brazilian cohort of adult and pediatric patients. Eur J Endocrinol 166: 61-77. doi:10.1530/EJE-11-0806. PubMed: 22048964.
23. Huang J, Zheng DL, Qin FS, Cheng N, Chen H et al. (2010) Genetic and epigenetic silencing of scavenger receptor SCARA5 as a candidate tumor suppressor could contribute to hepatocellular carcinoma via activation of FAK. J Clin Invest 120: 223-241. doi:10.1172/JCI38012. PubMed: 20038795.
24. Huang J, Zhang X, Zhang M, Zhu JD, Zhang YL et al. (2007) Upregulation of DLK1 gene could contribute to human hepatocellular carcinoma. Carcinogenesis 28: 1094-1103. PubMed: 17114643.
25. Zhao L, Qin LX, Ye QH, Zhu XQ, Zhang H et al. (2004) KIAA0008 gene is associated with invasive phenotype of human hepatocellular carcinoma - a functional analysis. J Cancer Res Clin Oncol 130: 719-727. doi:10.1007/s00432- 004-0595-2. PubMed: 15340842.