Improved liquid chromatography-tandem mass spectrometric method for the determination of ethyl glucuronide concentrations in hair: Applications to forensic cases

International Journal of Legal Medicine

Volumn 128, Issue 1, 2014, Pages 53-58

  Imbert, Laurent ^a   Gaulier, Jean Michel ^a   Dulaurent, Sylvain ^a   Morichon, Julien ^a   Bevalot, Fabien ^b   Izac, Paul ^c   Lachâtre, Gérard ^a  

^a LIMOGES UNIVERSITY HOSPITAL   (France)

^b Laboratoire LAT LUMTOX   (France)

^c Commission Médicale des Permis de Conduire Préfecture de la Haute Vienne ^*  (France)

Author keywords

Chronic alcoholism; Ethyl glucuronide; Forensic; Hair; Mass spectrometry; Teetotaller

Indexed keywords

ETHYL GLUCURONIDE; GLUCURONIC ACID;

ALCOHOL ABSTINENCE; ALCOHOLISM; ARTICLE; CHEMISTRY; DRINKING BEHAVIOR; HAIR; HUMAN; LEGAL ASPECT; LIQUID CHROMATOGRAPHY; METHODOLOGY; PREDICTIVE VALUE; REFERENCE VALUE; TANDEM MASS SPECTROMETRY;

ALCOHOL DRINKING; ALCOHOLISM; CHROMATOGRAPHY, LIQUID; GLUCURONATES; HAIR; HUMANS; PREDICTIVE VALUE OF TESTS; REFERENCE VALUES; TANDEM MASS SPECTROMETRY; TEMPERANCE;

EID: 84891825072     PISSN: 09379827     EISSN: 14371596     Source Type: Journal    
DOI: 10.1007/s00414-013-0894-8     Document Type: Article

References (29)

1. Hart CL, Smith GD (2008) Alcohol consumption and mortality and hospital admissions in men from the Midspan collaborative cohort study. Addiction 103:1979-1986
2. De Wildt SN, Kearns GL, Leeder JS, Van den Anker JN (1999) Glucuronidation in humans. Pharmacogenetic and developmental aspects. Clin Pharmacokinet 36:439-452
3. Foti RS, Fisher MB (2005) Assessment of UDP-glucuronosyltransferase catalyzed formation of ethyl glucuronide in human liver microsomes and recombinant UGTs. Forensic Sci Int 153:109-116
4. Pirro V, Di Corcia D, Pellegrino S, Vincenti M, Sciutteri B, Salomone A (2011) A study of distribution of ethyl glucuronide in different keratin matrices. Forensic Sci Int 210:271-277
5. Dahl H, Stephanson N, Beck O, Helander A (2002) Comparison of urinary excretion characteristics of ethanol and ethyl glucuronide. J Anal Toxicol 26:201-204
6. Al Saabi A, Allorge D, Sauvage FL, Tournel G, Gaulier JM, Marquet P, Picard N (2013) Involvement of UDP-Glucuronosyltransferases UGT1A9 and UGT2B7 in ethanol glucuronidation, and interactions with common drugs of abuse. Drug Metab Dispos 41:568-574
7. Wurst FM, Skipper GE, Weinmann W (2003) Ethyl glucuronide - the direct ethanol metabolite on the threshold from science to routine use. Addiction 98(Suppl 2):51-61
8. Liniger B, Nguyen A, Friedrich-Koch A, Yegles M (2010) Abstinence monitoring of suspected drinking drivers: ethyl glucuronide in hair versus CDT. Traffic Inj Prev 11:123-126
9. Joya X, Friguls B, Ortigosa S, Papaseit E, Martínez SE, Manich A, Garcia-Algar O, Pacifici R, Vall O, Pichini S (2012) Determination of maternal-fetal biomarkers of prenatal exposure to ethanol: a review. J Pharm Biomed Anal 69:209-222
10. Høiseth G, Morini L, Polettini A, Christophersen A, Mørland J (2009) Ethyl glucuronide in hair compared with traditional alcohol biomarkers - a pilot study of heavy drinkers referred to an alcohol detoxification unit. Alcohol Clin Exp Res 33:812-816
11. Morini L, Politi L, Acito S, Groppi A, Polettini A (2009) Comparison of ethyl glucuronide in hair with carbohydrate-deficient transferrin in serum as markers of chronic high levels of alcohol consumption. Forensic Sci Int 188:140-143
12. Society of Hair Testing (2009) Consensus of the Society of Hair Testing on hair testing for chronic excessive alcohol consumption. http://www.soht.org/ pdf/Consensus-EtG-2009.pdf. Accessed 18 March 2013
13. Society of Hair Testing (2011) Consensus of the Society of Hair Testing on hair testing for chronic excessive alcohol consumption. http://www.soht.org/ pdf/Revised%20Alcohol%20marker%20Consensus.pdf. Accessed 18 March 2013
14. Tsanaclis L, Kingston R, Wicks J (2009) Testing for alcohol use in hair: is ethyl glucuronide (EtG) stable in hair? Ann Toxicol Anal 21:67-71
15. Yegles M, Labarthe A, Auwärter V, Hartwig S, Vater H, Wennig R, Pragst F (2004) Comparison of ethyl glucuronide and fatty acid ethyl ester concentrations in hair of alcoholics, social drinkers and teetotallers. Forensic Sci Int 145:167-173
16. Agius R, Nadulski T, Kahl H-G, Schräder J, Dufaux B, Yegles M, Pragst F (2010) Validation of a headspace solid-phase microextraction-GC-MS/MS for the determination of ethyl glucuronide in hair according to forensic guidelines. Forensic Sci Int 196:3-9
17. Bendroth P, Kronstrand R, Helander A, Greby J, Stephanson N, Krantz P (2008) Comparison of ethyl glucuronide in hair with phosphatidylethanol in whole blood as post-mortem markers of alcohol abuse. Forensic Sci Int 176:76-81
18. Albermann ME, Musshoff F, Madea B (2012) A high-performance liquid chromatographic-tandem mass spectrometric method for the determination of ethyl glucuronide and ethyl sulfate in urine validated according to forensic guidelines. J Chromatogr Sci 50:51-56
19. Albermann ME, Mushoff F, Madea B (2010) A fully validated high-performance liquid chromatography-tandem mass spectrometry method for the determination of ethylglucuronide in hair for the proof of strict alcohol abstinence. Anal Bioanal Chem 396:2441-2447
20. Morini L, Varango C, Filippi C, Rusca C, Danesino P, Cheli F, Fusini M, Iannello G, Groppi A (2011) Chronic excessive alcohol consumption diagnosis: comparison between traditional biomarkers and ethyl glucuronide in hair, a study on a real population. Ther Drug Monit 33:654-657
21. Albermann ME, Musshoff F, Madea B (2010) A fully validated high-performance liquid chromatography-tandem mass spectrometry method for the determination of ethyl glucuronide in hair for the proof of strict alcohol abstinence. Anal Bioanal Chem 396:2441-2447
22. Lamoureux F, Gaulier JM, Sauvage FL, Mercerolle M, Vallejo C, Lachâtre G (2009) Determination of ethyl-glucuronide in hair for heavy drinking detection using liquid chromatography-tandem mass spectrometry following solid-phase extraction. Anal Bioanal Chem 394:1895-1901
23. Society of Hair Testing (2012) Use of Alcohol Markers in Hair for Abstinence Assessment. http://www.soht.org/pdf/ Use%20of%20Alcohol%20Markers%20in%20Hair%20for%20Abstinence%20Assessment%202012. pdf. Accessed 18 March 2013
24. Peters FT, Drummer OH, Musshoff F (2007) Validation of new methods. Forensic Sci Int 165:216-224
25. Musshoff F, Madea B (2007) New trends in hair analysis and scientific demands on validation and technical notes. Forensic Sci Int 165:204-215
26. Tarcomnicu I, Van Nuijs ALN, Aerts K, De Doncker M, Covaci A, Neels H (2010) Ethyl glucuronide determination in meconium and hair by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Forensic Sci Int 196:121-127
27. Cabarcos P, Hassan HM, Tabernero MJ, Scott KS (2012) Analysis of ethyl glucuronide in hair samples by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). J Appl Toxicol. doi: 10.1002/jat.1791
28. Morini L, Marchei E, Vagnarelli F, Garcia Algar O, Groppi A, Mastrobattista L, Pichini S (2010) Ethyl glucuronide and ethyl sulfate in meconium and hair-potential biomarkers of intrauterine exposure to ethanol. Forensic Sci Int 196:74-77
29. Mönch B, Becker R, Jung C, Nehls I (2013) The homogeneity testing of EtG in hair reference materials: a high-throughput procedure using GC-NCI-MS. Forensic Sci Int 226:202-207