Analysis of ethyl glucuronide in hair samples by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)

Journal of Applied Toxicology

Volumn 33, Issue 7, 2013, Pages 638-643

  Cabarcos, Pamela ^a   Hassan, Huda M ^b   Tabernero, María Jesús ^a   Scott, Karen S ^b  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Daughter ion; Direct phase II metabolite; Ethyl glucuronide; LC ESI MS MS; Precursor ion; Reverse phase; UDP glucuronosyl transferases

Indexed keywords

ACETONITRILE; ETHYL GLUCURONIDE; FORMIC ACID;

ARTICLE; AUTOPSY; CHEMICAL ANALYSIS; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; HAIR; HUMAN; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; PARTICLE SIZE; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; STANDARD; TANDEM MASS SPECTROMETRY;

ALCOHOLISM; BIOLOGICAL MARKERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUCURONATES; HAIR; HUMANS; INDICATORS AND REAGENTS; LIMIT OF DETECTION; QUALITY CONTROL; REFERENCE STANDARDS; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SOLID PHASE EXTRACTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84878200809     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.1791     Document Type: Article

References (22)

1. Albermann ME, Musshoff E, Madea B. 2010. A fully validated high-performance liquid chromatography-tandem mass spectrometry method for the determination of ethyl glucuronide in hair for the proof of strict alcohol abstinence. Anal. Bioanal. Chem. 396: 2441-2447.
2. Alt A, Janda I, Seidl S, Wurst F. 2000. Determination of ethyl glucuronide in hair samples. Alcohol Alcohol. 35(3): 313-314.
3. Álvarez I, Bermejo AM, Tabernero MJ, Fernández P, Cabarcos P, López P. 2009. Microwave-assisted extraction: a simpler and faster method for the determination of ethyl glucuronide in hair by gas chromatography-mass spectrometry. Anal. Bioanal. Chem. 393: 1345-1350.
4. Appenzeller BMR, Schuman M, Yegles M, Wennig R. 2007. Ethyl glucuronide concentration in hair is not influenced by pigmentation. Alcohol Alcohol. 42(4): 326-327.
5. Bendroth P, Kronstrand R, Helander A, Greby J, Stephanson N, Krantz P. 2008. Comparison of ethyl glucuronide in hair with phosphatidylethanol in whole blood as post-mortem markers of alcohol abuse. For. Sci. Int. 176: 76-81.
6. FDA. 2001. Guidance for Industry, Bioanalytical Method Validation. Food and Drug Administration. Available at: http://www.fda.gov/cder/guidance/4252fnl.pdf].
7. Janda I, Weinmann W, Kuehnle T, Lahode M, Alt A. 2002. Determination of ethyl glucuronide in human hair by SPE and LC-MS/MS. For. Sci. Int. 128: 59-65.
8. Kharbouche H, Sporkert F, Troxler S, Augsburger M, Mangin P, Staub C. 2009. Development and validation of a gas chromatography-negative chemical ionization tandem mass spectrometry method for the determination of ethyl glucuronide in hair and its application to forensic toxicology. J. Chromatogr. B 877: 2337-2343.
9. Kharbouche H, Steiner N, Morelato M, Staub C, Boutrel B, Mangin P, Sporkert F, Augsburge A. 2010. Influence of etanol dose and pigmentation on the incorporation of ethyl glucuronide into rat hair. Alcohol 44(6): 507-514.
10. Kintz P, Villain M, Vallet E, Etter M, Salquebre G, Cirimele V. 2008. Ethyl glucuronide: Unusual distribution between head hair and pubic hair. For. Sci. Int. 176: 87-90.
11. Klys M, Scislowski M, Rojek S, Kolodziej J. 2005. A fatal clomipramine intoxication case of a chronic alcoholic patient: application of post-mortem hair analysis method of clomipramine and ethyl glucuronide using LC/APCI/MS. Legal Med. 7: 319-325.
12. Lamoureux F, Gaullier JM, Sauvage FL, Mercerolle M, Vallejo C, Lachatre G. 2009. Determination of ethyl-glucuronide in hair for heavy drinking detection using liquid chromatography-tandem mass spectrometry following solid-phase extraction. Anal. Bioanal. Chem. 394: 1895-1901.
13. Matuszewski BK, Constanzer ML, Chavez-Eng CM. 2003. Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Anal. Chem. 75: 3019-3030.
14. Morini L, Politi L, Groppi A, Stramesi C, Polettini A. 2006. Determination of ethyl glucuronide in hair samples by liquid chromatography/electrospray tandem mass spectrometry. J. Mass Spectrom. 41: 34-42.
15. Morini L, Politi L, Polettini A. 2009. Ethyl glucuronide in hair. A sensitive and specific marker of chronic heavy drinking. Addiction 104: 915-920.
16. Morini L, Zucchella A, Polettini A, Politi L, Groppi A. 2010. Effect of bleaching on ehtyl glucuronide in hair: an in vitro experiment. For. Sci. Int. 198: 23-27.
17. Paul R, Kingston R, Tsanaclis L, Berry A, Guwy A. 2008. Do drug users use less alcohol than non-drug users? A comparison of ethyl glucuronide concentration in hair between the two groups in medico-legal cases. For. Sci. Int. 176: 82-86.
18. Politi L, Morini L, Leone F, Polettini A. 2006. Ethyl glucuronide in hair: is it a reliable marker of chronic high levels of alcohol consumption? Addiction 101: 1408-1412.
19. Politi L, Morini L, Mari F, Groppi A, Bertol E. 2008. Ethyl glucuronide and ethyl sulphate in autopsy samples 27years after death. Int. J. Legal Med. 122: 507-509.
20. SoHT. 2009. Consensus of the Society of Hair Testing on Hair Testing for Chronic Excessive Alcohol Consumption.
21. Tarcomnicu T, Van Nuijs ALN, Aerts K, De Doncker M, Covaci A. 2010 Ethyl glucuronide in meconium and hair by hydrophilic interaction liquid chromatography-tandem mass spectrometry. For. Sci. Int. 196: 121-127.
22. Yegles M, Labarthe A, Auwärter V, Hartwig S, Vater H, Wennig R, Pragst F. 2004. Comparison of ethyl glucuronide and fatty acid ethyl ester concentrations in hair of alcoholic, social drinkers and teetotallers. For. Sci. Int. 145: 167-173.