A fully validated high-performance liquid chromatography-tandem mass spectrometry method for the determination of ethyl glucuronide in hair for the proof of strict alcohol abstinence

Analytical and Bioanalytical Chemistry

Volumn 396, Issue 7, 2010, Pages 2441-2447

  Albermann, Maria Elena ^a   Musshoff, F ^a   Madea, B ^a  

^a UNIVERSITY HOSPITAL BONN   (Germany)

Author keywords

Abstinence test; Driving ability; Ethyl glucuronide; Hair analysis; LC MS MS

Indexed keywords

CALIBRATION CURVES; CHROMATOGRAPHIC CONDITIONS; CUT-OFF VALUE; DETECTION AND QUANTIFICATION LIMIT; DRIVING ABILITIES; ETHYL GLUCURONIDE; FATTY ACID ETHYL ESTER; FORENSIC TOXICOLOGY; HAIR ANALYSIS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; LC-MS/MS;

CHROMATOGRAPHY; ESTERS; ETHANOL; FATTY ACIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY;

TESTING;

BIOLOGICAL MARKER; ETHYL GLUCURONIDE; GLUCURONIC ACID;

ALCOHOLISM; CHEMISTRY; CONFERENCE PAPER; DRINKING BEHAVIOR; DRIVER LICENCE; ELECTROSPRAY MASS SPECTROMETRY; EVALUATION; HAIR; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METABOLISM; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE; VALIDATION STUDY;

ALCOHOL DRINKING; ALCOHOLISM; AUTOMOBILE DRIVER EXAMINATION; BIOLOGICAL MARKERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUCURONATES; HAIR; HUMANS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTANCE ABUSE DETECTION;

EID: 77951256904     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-009-3388-2     Document Type: Conference Paper

References (30)

1. F Pragst MA Balikova 2006 State of the art in hair analysis for detection of drugs and alcohol abuse Clin Chim Acta 370 17 49 10.1016/j.cca.2006.02.019 1:CAS:528:DC%2BD28XntlWns7w%3D (Pubitemid 44137285)
2. F Musshoff B Madea 2007 Analytical pitfalls in hair testing Anal Bioanal Chem 388 1475 1494 10.1007/s00216-007-1288-x 1:CAS:528:DC%2BD2sXnsVeqtr0%3D (Pubitemid 47150874)
3. F Musshoff B Madea 2007 New trends in hair analysis and scientific demands on validation and technical notes Forensic Sci Int 165 204 215 10.1016/j.forsciint.2006.05.024 1:CAS:528:DC%2BD2sXltV2ksg%3D%3D (Pubitemid 46084889)
4. Madea B, F. Musshoff F (2004) Haaranalytik: Technik und Interpretation in Medizin und Recht. Deutscher Ärzte, Cologne
5. F Pragst M Yegles 2008 Determination of fatty acid ethyl esters (FAEE) and ethyl glucuronide (EtG) in hair: a promising way for retrospective detection of alcohol abuse during pregnancy? Ther Drug Monit 30 255 263 10.1097/FTD.0b013e318167d602 1:CAS:528:DC%2BD1cXjslSqurg%3D (Pubitemid 351521658)
6. M Laposata 1998 Fatty acid ethyl esters: ethanol metabolites which mediate ethanol-induced organ damage and serve as markers of ethanol intake Prog Lipid Res 35 307 316 10.1016/S0163-7827(98)00013-7 (Pubitemid 28442470)
7. F Pragst V Auwaerter F Spokert K Spiegel 2001 Analysis of fatty acid ethyl esters in hair as possible markers of chronically elevated alcohol consumption by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) Forensic Sci Int 121 76 88 10.1016/S0379-0738(01)00456-X 1:CAS:528:DC%2BD3MXmtV2nu74%3D (Pubitemid 32761728)
8. RS Foti MB Fisher 2005 Assessment of UDP-glucuronosyltransferase catalyzed formation of ethyl glucuronide in human liver microsomes and recombinant UGTs Forensic Sci Int 153 109 116 10.1016/j.forsciint.2004.12.003 1:CAS:528:DC%2BD2MXpslarsr4%3D (Pubitemid 41219660)
9. Society of Hair Testing Consensus of the Society of Hair Testing on hair testing for chronic excessive alcohol consumption (2009) http://www.soht.org/ pdf/Consensus-EtG-2009.pdf. Accessed 18.08.2009.
10. Schubert W, Mattern R (2009) Beurteilungskriterien zur Fahreignungsdiagnostik, 2nd edn. Kirschbaum, Bonn
11. Society of Toxicological and Forensic Chemistry (GTFCh) (2009) Richtlinie der GTFCh zur Qualitätssicherung bei forensisch-toxikologischen Untersuchungen. T +K 76:142-176
12. Society of Toxicological and Forensic Chemistry (GTFCh) Anforderungen an die Validierung von Analysenmethoden. T +K 76:185-208
13. Society of Toxicological and Forensic Chemistry (GTFCh) Anforderungen an die Untersuchung von Haarproben. T +K:76 209-216
14. FT Peters OH Drummer F Musshoff 2007 Validation of new methods Forensic Sci Int 165 216 224 10.1016/j.forsciint.2006.05.021 1:CAS:528: DC%2BD2sXltV2ksw%3D%3D (Pubitemid 46073540)
15. FT Peters HH Maurer 2002 Bioanalytical method validation and its implications for forensic and clinical toxicology-a review Accred Qual Assur 7 441 449 10.1007/s00769-002-0516-5 1:CAS:528:DC%2BD38Xos1Whu7o%3D (Pubitemid 36165305)
16. Society of Toxicological and Forensic Chemistry (GTFCh) Qualitätssicherung im forensisch-toxikologischen Labor und VALISTAT 1.0 http://www.ps-gtfch.de Accessed 13.08.2009
17. L Morini L Politi A Groppi C Stramesi A Polettini 2006 Determination of ethyl glucuronide in hair samples by liquid chromatography/electrospray tandem mass spectrometry J Mass Spectrom 41 34 42 10.1002/jms.943 1:CAS:528: DC%2BD28XhtFGitLo%3D (Pubitemid 43164832)
18. Deutsches Institut für Normung DIN 32645 Chemische Analytik -Nachweis- Erfassungs- und Bestimmungsgrenze unter Wiederholbedingungen-Begriffe Verfahren Auswertung (2008) http://www.nmp.din.de Accessed 18.08.2009
19. G Skopp G Schmitt L Pötsch P Drönner R Aderjan R Mattern 2000 Ethyl glucuronide in human hair Alcohol Alcohol 35 283 285 1:CAS:528: DC%2BD3cXlsFKktrk%3D (Pubitemid 30433701)
20. F Lamoureux JM Gaulier FL Sauvage M Mercerolle C Vallejo G Lachâtre 2009 Determination of ethyl-glucuronide in hair for heavy drinking detection using liquid chromatography-tandem mass spectrometry following solid-phase extraction Anal Bioanal Chem 394 1895 1901 10.1007/s00216-009-2863-0 1:CAS:528:DC%2BD1MXntV2jtLo%3D
21. L Politi L Morini F Leone A Polettini 2006 Ethyl glucuronide in hair: is it a reliable marker of chronic high levels of alcohol consumption? Addiction 101 1408 1412 10.1111/j.1360-0443.2006.01537.x (Pubitemid 44337764)
22. M Concheiro A Cruz M Mon A de Castro O Quintela A Lorenzo M López-Rivadulla 2009 Ethylglucuronide determination in urine and hair from alcohol withdrawal patients J Anal Toxicol 33 155 161 1:CAS:528: DC%2BD1MXks1SqsLg%3D
23. H Dahl N Stephanson O Beck A Helander 2002 Comparison of urinary excretion characteristics of ethanol and ethyl glucuronide J Anal Toxicol 26 201 204 1:CAS:528:DC%2BD38XktFyksrc%3D (Pubitemid 34563953)
24. A Alt I Janda S Seidl F Wurst 2000 Determination of ethyl glucuronide in hair samples Alcohol Alcohol 35 313 314 1:CAS:528:DC%2BD3cXlsFKkt78%3D (Pubitemid 30433706)
25. I Janda W Weinmann T Kuehnle M Lahode A Alt 2002 Determination of ethyl glucuronide in human hair by SPE and LC-MS/MS Forensic Sci Int 128 59 65 10.1016/S0379-0738(02)00163-9 1:CAS:528:DC%2BD38Xms12hsL8%3D
26. C Jurado T Soriano MP Giménez M Menédez 2004 Diagnosis of chronic alcohol consumption hair analysis for ethyl-glucuronide Forensic Sci Int 145 161 166 10.1016/j.forsciint.2004.04.031 1:CAS:528:DC%2BD2cXnvV2jt7w%3D (Pubitemid 39296634)
27. M Yegles A Labarthe V Auwärter S Hartwig H Vater R Wennig F Pragst 2004 Comparison of ethyl glucuronide and fatty acid ethyl ester concentrations in hair of alcoholics social drinkers and teetotallers Forensic Sci Int 145 167 173 10.1016/j.forsciint.2004.04.032 1:CAS:528:DC%2BD2cXnvV2jt70%3D (Pubitemid 39302536)
28. R Paul R Kingston L Tsanaclis A Berry A Guwy 2008 Do drug users use less alcohol than non drug users? A comparison of ethyl glucuronide concentrations in hair between the two groups in medico-legal cases Forensic Sci Int 176 82 86 10.1016/j.forsciint.2007.06.025 1:CAS:528:DC%2BD1cXitlers7g%3D (Pubitemid 351273946)
29. H Kharbouche F Spokert S Troxler M Augsburger P Mangin C Staub 2009 Development and validation of a gas chromatography-negative chemical ionization tandem mass spectrometry method for the determination of ethyl glucuronide in hair and its application to forensic toxicology J Chromatogr B 877 2337 2343 10.1016/j.jchromb.2008.11.046 1:CAS:528:DC%2BD1MXoslehu78%3D
30. I Álvarez AM Bermejo MJ Tabernero P Fernández P Cabarcos P López 2009 Microwave-assisted extraction: a simpler and faster method for the determination of ethyl glucuronide in hair by gas chromatography-mass spectrometry Anal Bioanal Chem 393 1345 1350 10.1007/s00216-008-2546-2