Preparation and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT)

Current Protocols in Stem Cell Biology

Volumn 1, Issue SUPPL.27, 2013, Pages

  Mirsaidi, Ali ^a   Tiaden, André N ^a   Richards, Peter J ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

3D culture; Adipose derived stromal cells; Osteogenesis

Indexed keywords

PARAFFIN; TISSUE SCAFFOLD;

ADIPOSE DERIVED STEM CELL; ADIPOSE TISSUE; ARTICLE; BONE DEVELOPMENT; CELL CULTURE; CELL DIFFERENTIATION; CELL POPULATION; CULTURE MEDIUM; EMBEDDING; HISTOLOGY; MINERALIZATION; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; STROMA CELL; TIME; 3D-CULTURE; ADIPOSE-DERIVED STROMAL CELLS; ANIMAL; CELL PROLIFERATION; CELL SEPARATION; CYTOLOGY; MALE; METHODOLOGY; MOUSE; MULTICELLULAR SPHEROID; TISSUE CULTURE TECHNIQUE;

3D-CULTURE; ADIPOSE-DERIVED STROMAL CELLS; OSTEOGENESIS;

ADIPOSE TISSUE; ANIMALS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELLS, CULTURED; MALE; MICE; OSTEOGENESIS; SPHEROIDS, CELLULAR; STROMAL CELLS; TISSUE CULTURE TECHNIQUES; TISSUE SCAFFOLDS;

EID: 84891671297     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc02b05s27     Document Type: Article

References (13)

1. Bokel, C. and Brown, N.H. 2002. Integrins in development: Moving on, responding to, and sticking to the extracellular matrix. Dev. Cell 3:311-321
2. Boudreau, N.J. 2003. Organized living: From cell surfaces to basement membranes. Sci STKE 2003:pe34
3. Dunlop, L.L. and Hall, B.K. 1995. Relationships between cellular condensation, preosteoblast formation and epithelial-mesenchymal interactions in initiation of osteogenesis. Int. J. Dev. Biol. 39:357-371
4. Friedrich, J., Seidel, C., Ebner, R., and Kunz-Schughart, L.A. 2009. Spheroid-based drug screen: Considerations and practical approach. Nat. Protoc. 4:309-324
5. Goldmann, W.H. 2002. Mechanical aspects of cell shape regulation and signaling. Cell Biol. Int. 26:313-317
6. Keller, G.M. 1995. In vitro differentiation of embryonic stem cells. Curr. Opin. Cell Biol. 7:862-869
7. Kelm, J.M. and Fussenegger, M. 2010. Scaffoldfree cell delivery for use in regenerative medicine. Adv. Drug Deliv. Rev. 62:753-764
8. Kelm, J.M., Timmins, N.E., Brown, C.J., Fussenegger, M., and Nielsen, L.K. 2003. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol. Bioeng. 83:173-180
9. Mirsaidi, A., Kleinhans, K.N., Rimann, M., Tiaden, A.N., Stauber, M., Rudolph, K.L., and Richards, P.J. 2012. Telomere length, telomerase activity and osteogenic differentiation are maintained in adipose-derived stromal cells from senile osteoporotic SAMP6 mice. J. Tissue Eng. Regen. Med. 6:378-390
10. Schenk, S. and Quaranta, V. 2003. Tales from the crypt[ic] sites of the extracellular matrix. Trends Cell Biol. 13:366-375
11. Tapp, H., Hanley, E.N. Jr., Patt, J.C., and Gruber, H.E. 2009. Adipose-derived stem cells: Characterization and current application in orthopaedic tissue repair. Exp. Biol. Med. 234:1-9
12. Tarone, G., Hirsch, E., Brancaccio, M., De Acetis, M., Barberis, L., Balzac, F., Retta, S.F., Botta, C., Altruda, F., and Silengo, L. 2000. Integrin function and regulation in development. Int. J. Dev. Biol. 44:725-731
13. Tiaden, A.N., Breiden, M., Mirsaidi, A., Weber, F.A., Bahrenberg, G., Glanz, S., Cinelli, P., Ehrmann, M., and Richards, P.J. 2012. Human serine proteaseHTRA1 positively regulates osteogenesis of human bone marrow-derived mesenchymal stem cells and mineralization of differentiating bone-forming cells through the modulation of extracellular matrix protein. Stem Cells 30:2271-2282