Effect of hepatitis B virus X gene on the expression level of p53 gene using hep G2 cell line

Avicenna Journal of Medical Biotechnology

Volumn 6, Issue 1, 2014, Pages 3-9

  Kordestani, Roghyeh ^a,b   Mirshafiee, Hamideh ^a,b   Hosseini, Seyed Masoud ^b   Sharifi, Zohreh ^a  

^a HIGH INSTITUTE FOR RESEARCH AND EDUCATION IN TRANSFUSION MEDICINE   (Iran)

^b SHAHID BEHESHTI UNIVERSITY   (Iran)

Author keywords

Hep G2 cell line; Hepatitis B virus; p53 gene; X gene

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEPATITIS B VIRUS X PROTEIN; PROTEIN P53; VIRUS DNA; VIRUS VECTOR;

ARTICLE; CELL CULTURE; CLONING; CONTROLLED STUDY; DNA EXTRACTION; DNA TRANSFECTION; GENE; GENE EXPRESSION; HEPATITIS B VIRUS X GENE; HUMAN; HUMAN CELL; MUTATION; P53 GENE; POLYACRYLAMIDE GEL ELECTROPHORESIS; REAL TIME POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID; RNA EXTRACTION; WESTERN BLOTTING;

EID: 84891655680     PISSN: 20082835     EISSN: 20084625     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Neuveut C, Wei Y, Buendia MA. Mechanisms of HBV-related hepatocarcinogenesis. J Hepatol 2010; 52(4):594-604.
2. Dewantoro O, Gani RA, Akbar N. Hepatocarcinogenesis in viral hepatitis B infection: The role of HBx and p53. Acta Med Indones 2006;38(3):154-159.
3. Kew MC. Epidemiology of hepatitis B virus infection, hepatocellular carcinoma, and hepatitis B virus-induced hepatocellular carcinoma. Pathol Biol 2010;58(4):273-277.
4. Kew MC. Hepatitis B virus x protein in the pathogenesis of hepatitis B virus-induced hepatocellular carcinoma. J Gastroenterol Hepatol 2011;26(Suppl 1):144-152.
5. Miller RH, Robinson WS. Common evolutionary origin of hepatitis x virus and retroviruses. Proc Natl Acad Sci USA 1986;83(8):2531-2535.
6. Lian Z, Liu J, Li L, Li X, Clayton M, Wu MC, et al. Enhanced cell survival of Hep3B cells by the hepatitis B x antigen effector, URG11, is associated with upregulation of beta-catenin. Hepatology 2006 43(3):415-424.
7. Lee JH, Han KH, Lee JM, Park HJ, Kim HS. Impact of hepatitis B virus (HBV) x gene mutations on hepatocellular carcinoma development in chronic HBV infection. Clin Vaccine Immunol 2011;18 (6):914-921.
8. Ghabeshi S, Sharifi Z, Hosseini SM, Mahmoodian Shooshtari M. Correlation between viral load of HBV in chronic hepatitis B patients and precore and basal core promoter mutations. Hepat Mon 2013;13(2):e7415.
9. Abdel-Hafiz HA. Role of hepatitis B virus x protein in DNA repair during hepatocellular carcinoma development. J Carcinogene Mutagene 2011;S3:001.
10. Qu ZL, Zou SQ, Cui NQ, Wu XZ, Qin MF, Kong D, et al. Upregulation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells. World J Gastroenterol 2005;2811(36):5627-5632.
11. Chen HY, Tang NH, Li XJ, Zhang SJ, Chen ZX, Wang XZ. Transfection and expression of hepatitis B virus x gene and its effect on apoptosis in HL-7702 cells. World J Gastroenterol 2004;10(7):959-964.
12. Ou DP, Tao YM, Tang FQ, Yang LY. The hepatitis B virus X protein promotes hepatocellular carcinoma metastasis by upregulation of matrix metalloproteinases. Int J Cancer 2007;120(6):1208-1214.
13. Lin N, Chen HY, Li D, Zhang SJ, Cheng ZX, Wang XZ. Apoptosis and its pathway in X genetransfected HepG2 cells. World J Gastroenterol 2005;11(28):4326-4331.
14. Vitvitski-Trépo L, Kay A, Pichoud C, Chevallier P, de Dinechin S, Shamoon BM, et al. Early and frequent detection of HBxAg and/or anti-HBx in he-patitis B virus infection. Hepatology 1990;12(6): 1278-1283.
15. Zhang H, Wu LY, Zhang S, Qiu LY, Li N, Zhang X, et al. Anti-hepatitis B virus x protein in sera is one of the markers of development of liver cirrhosis and liver cancer mediated by HBV. J Biomed Biotechnol 2009;2009:289068.
16. Hwang GY, Lin CY, Huang LM, Wang YH, Wang JC, Hsu CT, et al. Detection of the hepatitis B virus x protein (HBx) antigen and anti-HBx antibodies in cases of human hepatocellular carcinoma. J Clin Microbiol 2003;41(12):5598-5603.
17. Yun C, Lee JH, Park H, Jin YM, Park S, Park K, et al. Chemotherapeutic drug, adriamycin, restores the function of p53 protein in hepatitis B virus X (HBx) protein-expressing liver cells. Oncogene 2000;19(45):5163-5172.
18. Neuveut Ch, Wei Y, Buendia MA. Mechanisms of HBV-related hepatocarcinogenesis. J Hepatol 2010; 52(4):594-604.
19. Teodoro JG, Branton PE. Regulation of apoptosis by viral gene products. J Virol 1997;71(3):1739-1746.
20. Thomas M, Matlashewski G, Pim D, Banks L. Induction of apoptosis by p53 is independent of its oligomeric state and can be abolished by HPV-18 E6 through ubiquitin mediated degradation. Oncogene 1996;13(2):265-273.
21. Rubenwolf S, Schutt H, Nevels M, Wolf H, Dobner T. Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex. J Virol 1997;71(2):1115-1123.
22. Lin Y, Nomura T, Yamashita T, Dorjsuren D, Tang H, Murakami S. The transactivation and p53-interacting functions of hepatitis B virus X protein are mutually interfering but distinct. Cancer Res 1997;57(22):5137-5142.
23. Truant R, Antunovic J, Greenblatt J, Prives C, Chromlish JA. Direct interaction of the hepatitis B virus HBx protein with p53 leads to inhibition by HBx of p53 response element-directed transactivation. J Virol 1995;69(3):1851-1859.
24. Chung TW, Lee YC, Ko JH, Kim CH. Hepatitis B virus x protein modulates the expression of PTEN by inhibiting the function of p53, a transcriptional activator in liver cells. Cancer Res 2003;63(13): 3453-3458.
25. Lee AT, Ren J, Wong ET, Ban KH, Lee LA, Lee CG. The hepatitis B virus x protein sensitizes HepG2 cells to UV light-induced DNA damage. J Biol Chem 2005;280(39):33525-33535.