Detection of Aspergillus flavus in maize kernels by conventional and real-time PCR assays

International Food Research Journal

Volumn 20, Issue 6, 2013, Pages 3329-3335

  Shweta, S ^a   Madhavan, S ^a   Paranidharan, V ^a   Velazhahan, R ^a  

^a TAMIL NADU AGRICULTURAL UNIVERSITY   (India)

Author keywords

Aflatoxin; Aspergillus flavus; omt A gene; PCR detection; Zea mays

Indexed keywords

EID: 84891651944     PISSN: 19854668     EISSN: 22317546     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Bababunmi, E. A., Uwaifo A. O. and Bassir, O. 1978. Hepatocarcinogens in Nigerian food stuffs. World Review of Nutrition and Dietetics 28: 188-209.
2. Bennett, J. W. and Klich, M. 2003. Mycotoxins. Clinical Microbiology Reviews 16: 479-516.
3. Bhat, R. V., Vasanthi, S., Rao, B. S., Rao, R. N., Rao, V. S., Nagaraja, K. V., Bai, R. G., Prasad, C. A., Vanchinathan, S., Ray, R., Saha, S., Mukherjee, A., Ghosh, P. K., Toteja, G. S. and Saxena, B. W. 1997. Aflatoxin B1 contamination in maize samples collected from different geographical regions of India - a multicentre study. Food Additives and Contaminants 14: 151-156.
4. Burlakoti, R. R., Estrada, R. Jr., Rivera, V. V., Boddeda, A., Secor, G. A. and Adhikari, T. B. 2007. Real-time PCR quantification and mycotoxin production of Fusarium graminearum in wheat inoculated with isolates collected from potato, sugar beet, and wheat. Phytopathology 97: 835-841.
5. Calvert, O. H., Lillehoj, E. B., Kwolek, W. F. and Zuber, M. S. 1978. Aflatoxin B1 and G1 production in developing Zea mays kernels from mixed inocula of Aspergillus flavus and A. parasiticus. Phytopathology 68: 501-506.
6. Criseo, G., Bagnara, A. and Bisignano, G. 2001. Differentiation of aflatoxin-producing and non-producing strains of Aspergillus flavus group. Letters in Applied Microbiology 33: 291-295.
7. Cruz, P. and Buttner, M. P. 2008. Development and evaluation of a real-time quantitative PCR assay for Aspergillus flavus. Mycologia 100: 683-690.
8. Degola, F., Berni, E., Dall Asta, C.,Spotti, E., Marchelli, R., Ferrero, I. and Restivo, F. M. 2007. A multiplex RT-PCR approach to detect aflatoxigenic strains of Aspergillus flavus. Journal of Applied Microbiology 103: 409-417.
9. Doyle, J. J. and Doyle, J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
10. Gayoso, C., de Ilárduya, O. M., Pomar, F. and de Cáceres, F. M. 2007. Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars. European Journal of Plant Pathology 118: 199-209.
11. Geisen, R. 1998. PCR methods for the detection of mycotoxin producing fungi. In Bridge, P. D., Arora, D. K., Reddy, C. A. and Elander, R. P. (Eds.). Applications of PCR in mycology, p. 243-266. Wallingford - CAB International.
12. Kpodo, K. A.1996. Mycotoxins in maize and fermented maize products in southern Ghana. In Cardwell, K. F (Ed.). Proceedings of the workshop on mycotoxins in food in Africa, p. 33. Benin: International Institute of Tropical Agriculture.
13. Leisova, L., Kucera, L., Chrpova, J., Sykorova, S., Sip, V. and Ovesna, J. 2006. Quantification of Fusarium culmorum in wheat and barley tissues using Real-Time PCR in comparison with DON content. Journal of Phytopathology 154: 603-611.
14. Levin, R. E. 2012. PCR detection of aflatoxin producing fungi and its limitations. International Journal of Food Microbiology 156: 1-6.
15. Li, F. Q., Yoshizawa, T., Kawamura, O., Luo, X. Y. and Li, Y. W. 2001. Aflatoxins and fumonisins in corn from the high-incidence area for human hepatocellular carcinoma in Guangxi China. Journal of Agricultural and Food Chemistry 49: 4122-4126.
16. Lievens, B. and Thomma, B. P. H. J. 2005. Recent developments in pathogen detection arrays: Implications for fungal plant pathogens and use in practice. Phytopathology 95: 1374-1380.
17. Liu, D., Coloe, S., Baird, R. and Pederson, J. 2000. Rapid mini preparation of fungal DNA for PCR. Journal of Clinical Microbiology 38: 471.
18. Luque, M. I., Rodriguez, A., Andrade, M. J., Martin, A. and Cordoba, J. J. 2012. Development of a PCR protocol to detect aflatoxigenic molds in food products. Journal of Food Protection 75: 85-94.
19. Mayer, Z., Bagnara, A., Färber, P. and Geisen, R. 2003. Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods. International Journal of Food Microbiology 82: 143-151.
20. Moreno, O. J. and Kang, M. S. 1999. Aflatoxins in maize: The problem and genetic solutions. Plant Breeding 118: 1-16.
21. Ong, T. M. 1975. Aspergillus flavus mutagenesis. Mutation Research 32: 35-53.
22. Passone, M. A., Rosso, L. C., Ciancio, A. and Etcheverry, M. 2010. Detection and quantification of Aspergillus section Flavi spp. in stored peanuts by real-time PCR of nor-1 gene, and effects of storage conditions on aflatoxin production. International Journal of Food Microbiology 138: 276-281.
23. Polan, C. E., Hayes, J. R. and Campbell, T. C. 1974. Consumption and fate of aflatoxin B1 by lactacting cows. Journal of Agricultural and Food Chemistry 22: 635-638.
24. Reddy, S. V., Mayi, D. K., Reddy, M. U., Thirumala Devi, K. and Reddy, D. V. 2001. Aflatoxins B1 in different grades of chillies (Capsicum annuum L.) in India as determined by indirect competitive ELISA. Food Additives and Contaminants 18: 553-558.
25. Rodríguez, A., Luque, M. I., Andrade, M. I., Rodríguez, M., Asensio, M. A. and Cordoba, J. J. 2011a. Development of real-time PCR methods to quantify patulin-producing molds in food products. Food Microbiology 28: 1190-1199.
26. Rodríguez, A., Rodríguez, M., Luque, M. I., Justensen, A. F. and Cordoba, J. J. 2011b. Quantification of ochratoxin A-producing molds in food products by SYBR Green and TaqMan real-time PCR methods. International Journal of Food Microbiology 149: 226-235.
27. Rodríguez, A., Rodríguez, M., Luque, M. I., Martín, A. and Córdoba, J. J. 2012. Real-time PCR assays for detection and quantification of aflatoxin-producing molds in foods. Food Microbiology 31: 89-99.
28. Sambrook, J. and Russell, D. W. 2001. Molecular Cloning: a Laboratory Manual. 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
29. Setamou, M., Cardwell, K. F., Schulthess, F. and Hell, K. 1997. Aspergillus flavus infection and aflatoxin contamination of preharvest maize in Benin. Plant Disease 81: 1323-1327.
30. Shapira, R., Paster, N., Eyal, O., Menasherov, M., Matt, A. and Salomon, R. 1996. Detection of aflatoxigenic molds in grains by PCR. Applied and Environmental Microbiology 62: 3270-3273.
31. Sweeney, M. J., Pamies, P. and Dobson, A. D. W. 2000. The use of reverse transcription-polymerase chain reaction (RT-PCR) for monitoring aflatoxin production in Aspergillus parasiticus 439. International Journal of Food Microbiology 56: 97-103.
32. Trucksess, M. W., Stack, M. E., Nesheim, S., Albert, R. H. and Romer, T. R. 1994. Multifunctional column coupled with liquid chromatography for determination of aflatoxins B1, B2, G1 and G2 in corn, almonds, brazilnuts, peanuts and pistachio nuts: collaborative study. Journal of AOAC International 77: 1512-1521.
33. Ventura, M., Guillen, D., Anaya, I., Broto-Puig, F., Lliberia, J. L., Agut, M. and Comellas, L. 2006. Ultra-performance liquid chromatography/tandem mass spectrometry for the simultaneous analysis of aflatoxins B1, G1, B2, G2 and ochratoxin A in beer. Rapid Communications in Mass Spectrometry 20: 3199-3204.
34. Waliyar, F., Reddy, S. V., Subramaniam, K., Reddy, T. Y., Ramadevi, K., Craufurd, P. Q. and Wheeler, T. R. 2003. Importance of mycotoxins in food and feed in India. Aspects of Applied Biology 68: 147-154.
35. Whitaker, T., Horwitz, W., Albert, R. and Nesheim, S. 1996. Variability associated with analytical methods used to measure aflatoxin in agricultural commodities. Journal of AOAC International 79: 476-485.
36. Yu, J., Chang, P. K., Payne, G. A., Cary, J. W., Bhatnagar, D. and Cleveland, T. E. 1995. Comparison of the omtA genes encoding O-methyltransferases involved in aflatoxin biosynthesis from Aspergillus parasiticus and A. flavus. Gene 163: 121-125.