An improved Escherichia coli strain to host gene regulatory networks involving both the AraC and LacI inducible transcription factors

Journal of Biological Engineering

Volumn 8, Issue 1, 2014, Pages

  Kogenaru, Manjunatha ^a,b,c   Tans, Sander J ^a  

^a FOM INSTITUTE AMOLF   (Netherlands)

^b CENTRE FOR GENOMIC REGULATION CRG   (Spain)

^c UNIVERSITAT POMPEU FABRA   (Spain)

Author keywords

Escherichia coli; Gene network; Regulatory cascade; Repressor; Strain; Transcription factor

Indexed keywords

ARAC PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR LACL; UNCLASSIFIED DRUG; CHLORAMPHENICOL ACETYLTRANSFERASE; TISSUE FACTOR PATHWAY INHIBITOR;

BACTERIAL STRAIN; BIOENGINEERING; CONTROLLED STUDY; ESCHERICHIA COLI; GENE REGULATORY NETWORK; HOST; LACTOSE OPERON; LETTER; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; ARTICLE; DNA FLANKING REGION; GENOTYPE; POLYMERASE CHAIN REACTION; PROTEIN FUNCTION; SYNTHETIC BIOLOGY; WESTERN BLOTTING;

ESCHERICHIA COLI;

EID: 84891640423     PISSN: None     EISSN: 17541611     Source Type: Journal    
DOI: 10.1186/1754-1611-8-2     Document Type: Letter

References (9)

1. Gardner TS, Cantor CR, Collins JJ. Construction of a genetic toggle switch in Escherichia coli. Nature 2000, 403:339-342. 10.1038/35002131, 10659857.
2. Elowitz MB, Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000, 403:335-338. 10.1038/35002125, 10659856.
3. Kwok R. Five hard truths for synthetic biology. Nature 2010, 463:288-290. 10.1038/463288a, 20090726.
4. Tan C, Marguet P, You L. Emergent bistability by a growth-modulating positive feedback circuit. Nat Chem Biol 2009, 5:842-848. 10.1038/nchembio.218, 2908482, 19801994.
5. Siegele DA, Hu JC. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci USA 1997, 94:8168-8172. 10.1073/pnas.94.15.8168, 21575, 9223333.
6. Khlebnikov A, Datsenko KA, Skaug T, Wanner BL, Keasling JD. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology 2001, 147:3241-3247.
7. Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 2000, 97:6640-6645. 10.1073/pnas.120163297, 18686, 10829079.
8. Casadaban MJ, Cohen SN. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 1980, 138:179-207. 10.1016/0022-2836(80)90283-1, 6997493.
9. Kudla G, Murray AW, Tollervey D, Plotkin JB. Coding-sequence determinants of gene expression in Escherichia coli. Science 2009, 324:255-258. 10.1126/science.1170160, 19359587.