Optimization of PCR for quantification of simian immunodeficiency virus genomic RNA in plasma of rhesus macaques (Macaca mulatta) using armored RNA

Journal of Medical Primatology

Volumn 43, Issue 1, 2014, Pages 31-43

  Monjure, C J ^a   Tatum, C D ^a   Panganiban, A T ^a,b   Arainga, M ^a,d   Traina Dorge, V ^a,b   Marx, P A ^a,c   Didier, E S ^a,c  

^a TULANE NATIONAL PRIMATE RESEARCH CENTER   (United States)

^b TULANE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c TULANE UNIVERSITY   (United States)

^d UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

AIDS; Minimum information for publication of quantitative real time PCR experiments; PCR inhibition; Plasma viral load; Quantitative polymerase chain reaction

Indexed keywords

ARMORED RNA; COMPLEMENTARY DNA; GENOMIC RNA; PROTEINASE K; UNCLASSIFIED DRUG; VIRUS RNA;

ARTICLE; BRANCHED DNA SIGNAL AMPLIFICATION ASSAY; CALIBRATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA SYNTHESIS; ELUTION; ENZYME DEGRADATION; GENE; GENE AMPLIFICATION; GROUP SPECIFIC ANTIGEN GENE; HEPATITIS C VIRUS; HYDROLYSIS; LIMIT OF DETECTION; MACACA; NONHUMAN; PLASMA VOLUME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; RNA EXTRACTION; RNA ISOLATION; RNA SEQUENCE; SENSITIVITY ANALYSIS; SIMIAN IMMUNODEFICIENCY VIRUS;

HEPATITIS C VIRUS; MACACA; MACACA MULATTA; SIMIAN IMMUNODEFICIENCY VIRUS;

AIDS; MINIMUM INFORMATION FOR PUBLICATION OF QUANTITATIVE REAL-TIME PCR EXPERIMENTS; PCR INHIBITION; PLASMA VIRAL LOAD; QUANTITATIVE POLYMERASE CHAIN REACTION;

ANIMALS; GENOME, VIRAL; MACACA MULATTA; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SIMIAN ACQUIRED IMMUNODEFICIENCY SYNDROME; SIMIAN IMMUNODEFICIENCY VIRUS; VIRAL LOAD;

EID: 84891626759     PISSN: 00472565     EISSN: 16000684     Source Type: Journal    
DOI: 10.1111/jmp.12088     Document Type: Article

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