Identification of lactobacilli from deep carious lesions by means of species-specific PCR and MALDI-TOF mass spectrometry

Clinical Laboratory

Volumn 59, Issue 11-12, 2013, Pages 1373-1379

  Callaway, Angelika ^a   Kostrzewa, Markus ^b   Willershausen, Brita ^a   Schmidt, Frank ^c   Thiede, Bernd ^d   Küpper, Harald ^e   Kneist, Susanne ^e  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b BRUKER DALTONIK GMBH   (Germany)

^c UNIVERSITY OF GREIFSWALD   (Germany)

^d UNIVERSITY OF OSLO   (Norway)

^e Center of Dentistry   (Germany)

Author keywords

Hard and soft carious dentin; Identification; Lactobacillus species; MALDI TOF MS; Species specific PCR

Indexed keywords

RNA 16S;

AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CHILD; CONTROLLED STUDY; DATA BASE; DENTAL CARIES; DENTIN; HABITAT; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LACTOBACILLUS; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS GASSERI; LACTOBACILLUS PARACASEI; LACTOBACILLUS PLANTARUM; LACTOBACILLUS RHAMNOSUS; MASS SPECTROMETER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLAR TOOTH; NONHUMAN; POLYMERASE CHAIN REACTION; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE;

BASE SEQUENCE; DENTAL CARIES; DNA PRIMERS; HUMANS; LACTOBACILLUS; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84891606312     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: 10.7754/Clin.Lab.2013.121225     Document Type: Article

References (28)

1. Kandler O, Weiss N. Regular, nonsporing Gram-positive rods. In: Sneath PHA, Mair N, Sharpe ME, Holt JG (eds). Bergey's manual of systematic bacteriology. Baltimore: Williams & Wilkins, 1986, vol 2, pp 1208-34.
2. Gilliland ST. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Rev 1990;87:175-88.
3. Gasser F. Safety of lactic acid bacteria and their occurrence in human clinical infections. Bull Inst Pasteur 1994;92:45-67. (Pubitemid 24164994)
4. Klinke T, Kneist S, de Soet JJ, et al. Acid production by oral strains of Candida albicans and lactobacilli. Caries Res 2009;43:83-91.
5. Badet C, Thebaud NB. Ecology of lactobacilli in the oral cavity: a review of literature. Open Microbiol J 2008;2:38-48.
6. Caufield PW, Li Y, Dasanayake A, Saxena D. Diversity of lactobacilli in the oral cavities of young women with dental caries. Caries Res 2007;41:2-8.
7. Sookkhee S, Chulasiri M, Prachyabrued W. Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens. J Appl Microbiol 2001;90:172-9. (Pubitemid 32158736)
8. Marchant S, Brailsford SR, Twomey AC, Roberts GJ, Beighton D. The predominant microflora of nursing caries lesions. Caries Res 2001;35:397-406. (Pubitemid 33636858)
9. Becker MR, Paster BJ, Leys EJ, et al. Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol 2002;40:1001-9. (Pubitemid 34208659)
10. Byun R, Nadkami MA, Chhour KL, Martin FE, Jacques NA, Hunter N. Quantitative analysis of diverse Lactobacillus species present in advanced dental caries. J Clin Microbiol 2004;42:3128-36. (Pubitemid 38938069)
11. Munson MA, Banerjee A, Watson TF, Wade WG. Molecular analysis of the microflora associated with dental caries. J Clin Microbiol 2004;42:3023-9. (Pubitemid 38938052)
12. Chhour KL, Nadkarni MA, Byun R, Martin FE, Jacques NA, Hunter N. Molecular analysis of microbial diversity in advanced caries. J Clin Microbiol 2005;43:843-9. (Pubitemid 40256044)
13. Wright JT, Cutter GR, Dasanyanake AP, Stiles HM, Caufield PW. Effect of conventional dental restorative treatment on bacteria in saliva. Community Dent Oral Epidemiol 1992;20:138-43.
14. Wicht MJ, Haak R, Schutt-Gerowitt H, Kneist S, Noack MJ. Suppression of caries-related microorganisms in dentine lesions after short-term chlorhexidine or antibiotic treatment. Caries Res 2004;38:436-41. (Pubitemid 39194318)
15. Aguirre M, Collins MD. Lactic acid bacteria and human clinical infection. J Appl Bact 1993;75:95-107. (Pubitemid 23244069)
16. Cannon JP, Lee TA, Bolanos JT, Danziger LH. Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases. Eur J Microbiol Infect Dis 2005;24:31-40. (Pubitemid 40179129)
17. Heinrich R, Kneist S, Künzel W. A clinical controlled trial of indirect pulp capping on deciduous molars. Dtsch Zahnärztl Z 1991;46:581-3.
18. Ward LJH, Timmins MJ. Differentiation of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus by polymerase chain reaction. Lett Appl Microbiol 1999;29:90-2. (Pubitemid 29423821)
19. Tamrakar R, Yamada T, Furuta I, et al. Association between Lactobacillus species and bacterial vaginosis-related bacteria, and bacterial vaginosis scores in pregnant Japanese women. BMC Infect Dis 2007;7:128. Published online 2007 November 7. doi: 10.1186/1471-2334-7-128.
20. Walter J, Tannock GW, Tilsala-Timisjarvi A, et al. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 2000;66:297-303. (Pubitemid 30030531)
21. Klocke M, Mundt K. Development of a 16S rDNA-targeted PCR assay for monitoring of Lactobacillus plantarum and Lact. rhamnosus during co-cultivation for production of inoculants for silages. Lett Appl Microbiol 2004;39:267-73. (Pubitemid 39209096)
22. Eigner U, Holfelder M, Oberdorfer K, Betz-Wild U, Bertsch D, Fahr AM. Performance of a matrix-associated laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin Lab 2009;55:289-96.
23. Seng P, Drancourt M, Gouriet F, et al. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009;49:543-51.
24. Dušková M, Šedo O, Kšiková K, Zdráhal Z, Karpišková R. Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS. Int J Food Microbiol 2012;159:107-114.
25. Farrow JAE, Phillips BA, Collins MD. Nucleic acid studies on some heterofermentative lactobacilli: description of Lactobacillus malefermentans sp. nov. and Lactobacillus parabuchneri sp. nov. FEMS Microbiol Lett 1988;55:163-8. (Pubitemid 18267907)
26. Edwardsson S. Bacteriological studies on deep areas of carious dentine. Odontol Rev 1974;35(Suppl 32):1-143.
27. Botha SJ, Boy SC, Botha FS, Senekal R. Lactobacillus species associated with active caries lesions. J Dent Assoc S Afr 1998;53:3-6.
28. Bjørndal L, Larsen T. Changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure. Caries Res 2000;34(6):502-8.